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2014 | 36 | 11 |

Tytuł artykułu

Production of salidroside and polysaccharides in Rhodiola sachalinensis using airlift bioreactor systems

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Rhodiola sachalinensis is widely used in traditional Chinese medicine, and salidroside and polysaccharides are its important bioactive compounds. This study used airlift bioreactor systems to produce mass bioactive compounds through callus culture. Several factors affecting callus biomass and bioactive compound accumulation were investigated. Callus growth was vigorous in a bioreactor system, and the growth ratio was 2.8-fold higher in bioreactor culture than in agitated-flask culture. Callus biomass and polysaccharide content were favorable at 0.1 air volume per culture volume per min (vvm), whereas favorable salidroside content was observed at a high air volume (0.2 vvm). The maximum yields of salidroside (7.90 mg l⁻¹) and polysaccharide (2.87 g l⁻¹) were obtained at 0.1 vvm. Inoculum density greatly affected callus biomass and bioactive compound accumulation, and the highest biomass and contents or yields of salidroside and polysaccharide were determined at a high inoculum density of 12.5 g l⁻¹. The level of hydrogen ion concentration (pH) at 5.8 improved callus biomass accumulation. Acidic medium (pH 4.8) stimulated salidroside synthesis but higher pH level (7.8) promoted polysaccharide accumulation. The highest yields of both bioactive compounds were obtained at pH 5.8. Methyl jasmonate (MeJA) participated in synthesis promotion of bioactive compounds, and the contents and yields of salidroside [4.75 mg g⁻¹ dry weight (DW), 58.43 mg l⁻¹] and polysaccharides (392.41 mg g⁻¹ DW, 4.79 g l⁻¹) were at maximum at 125 and 150 lmol of MeJA. Therefore, bioreactor systems can be used to produce R. sachalinensis bioactive compounds, and callus culture in a bioreactor can be as an alternative method for supplying materials for commercial drug production.

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Opis fizyczny



  • Key Laboratory of Nature Resource, Changbai Mountain and Functional Molecular (Yanbian University), Ministry of Education, Park Road 977, Yanji, 133002, Jilin, China
  • Key Laboratory of Nature Resource, Changbai Mountain and Functional Molecular (Yanbian University), Ministry of Education, Park Road 977, Yanji, 133002, Jilin, China
  • Department of Horticulture Science, Chungbuk National University, Cheongju, 361-763, Republic of Korea
  • Key Laboratory of Nature Resource, Changbai Mountain and Functional Molecular (Yanbian University), Ministry of Education, Park Road 977, Yanji, 133002, Jilin, China
  • Key Laboratory of Nature Resource, Changbai Mountain and Functional Molecular (Yanbian University), Ministry of Education, Park Road 977, Yanji, 133002, Jilin, China


  • Ali M, Abbasi BH, Ihsanul H (2013) Production of commercially important secondary metabolites and antioxidant activity in cell suspension cultures of Artemisia absinthium L. Ind Crop Prod 49:400–406
  • Andreazza NL, Abreu IN, Sawaya AC, Eberlin MN, Mazzafera P (2009) Production of imidazole alkaloids in cell cultures of jaborandi as affected by the medium pH. Biotechnol Lett 31:607–614
  • Bais HP, Sudha G, George J, Ravishankar GA (2001) Influence of exogenous hormones on growth and secondary metabolite production in hairy root cultures of Cichorium intybus L. cv. Lucknow Local. In Vitro Cell Dev Plant 37:293–299
  • Baque MA, Shiragi MHK, Moh SH, Lee EJ, Paek KY (2013) Production of biomass and bioactive compounds by adventitious root suspension cultures of Morinda citrifolia (L.) in a liquidphase airlift balloon-type bioreactor. In Vitro Cell Dev Biol Plant 49:737–749
  • Bhatia P, Ashwath N (2005) Effect of medium pH on shoot regeneration from the cotyledonary explants of tomato. Biotechnology 4:7–10
  • Cheng XJ, Di L, Wu Y, Zhao QC, Du GZ, Liu YQ (1993) Studies on the hypoglycemic effect of Rhodiola sachalinensis A. Bor. Polysaccharides. Chin J Chin Mater Med 18:557–559, 575
  • Choi SM, Son SH, Yun SR, Kwon OW, Seon JH, Paek KY (2000) Pilot-scale culture of adventitious roots of ginseng in a bioreactor system. Plant Cell Tissue Organ Cult 62:187–193
  • Cui XH, Murthy HN, Wu CH, Paek KY (2010) Adventitious root suspension cultures of Hypericum perforatum: effect of nitrogen source on production of biomass and secondary metabolites. In Vitro Cell Dev Plant 46:437–444
  • Cui HY, Baque MA, Lee EJ, Paek KY (2013) Scale-up of adventitious root cultures of Echinacea angustifolia in a pilotscale bioreactor for the production of biomass and caffeic acid derivatives. Plant Biotechnol Rep 7:297–308
  • Cui HY, Murthy HN, Moh SH, Cui YY, Lee EJ, Paek KY (2014) Production of biomass and bioactive compounds in protocorm cultures of Dendrobium candidum Wall ex Lindl. using balloon type bubble bioreactors. Ind Crop Prod 53:28–33
  • Dubios M, Gilles K, Hamilton J, Rebers P, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
  • Fischer U, Alfermann AW (1995) Cultivation of photoautotrophic plant cell suspension in the bioreactor: influence of culture conditions. J Biotechnol 41:19–28
  • Gao JW, Lee JM (1992) Effect of oxygen supply on the suspension cultures of genetically modified tobacco cell. Biotechnol Prog 8:285–290
  • Guo XH, Gao WY, Xiao PG (2005) Factors affecting root growth and metabolite production in Salvia miltiorrhiza adventitious root cultures. Minerva Biotechnol 17:133–139
  • Ketchum REB, Gibson DM, Croteau RB, Shuler ML (1999) The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate. Biotechnol Bioeng 62:97–105
  • Kim SR, Choi JL, Costa MA, An G (1992) Identification of G-Box sequence as an essential element for methyl jasmonate response of potato proteinase Inhibitor II promoter. Plant Physiol 99:627–631
  • Kin YS, Hahn E, Murthy H, Paek K (2004) Adventitious root growth and ginsenoside accumulation in Panax ginseng cultures as affected by methyl jasmonate. Biotechnol Lett 26:1619–1622
  • Lee EJ, Mobin M, Hahn EJ, Paek KY (2006) Effects of sucrose, inoculum density, auxins, and aeration volume on cell growth of Gymnema sylvestre. J Plant Biol 49:427–431
  • Lee EJ, Moh SH, Paek KY (2011) Influence of inoculum density and aeration volume on biomass and bioactive compound production in bulb-type biore-actor cultures of Eleutherococcus koreanum Nakai. Bioresource Technol 2011:7165–7170
  • Li M, Hirata Y, Xu G, Niwa M (1990) Determination of polysaccharide contents in the drugs of Dendrobium. China Tradit Herb Drugs 21:10–12
  • Luthfi AMS, Chan LK, Boey PL (2004) Effect of cell source and pH of culture medium on the production of canthin-6-one alkaloids from the cell cultures of Tongkat Ali (Eurycoma longifolia Jack). J Plant Biotechnol 6:125–130
  • McClelland MT, Smith MAL (1990) Vessel type, closure, and explant orientation influence in vitro performance of five woody species. HortScience 25:797–800
  • Meijer JJ, Hoopen HJG, Libbenga KR (1993) Effects of hydrodynamic stress on cultured plant cell: a literature survey. Enzyme Microb Tech 15:234–238
  • Meng QY, Jian ML, Zhong WT (1994) Controlling the root-rot disease of Rhodiola sachalinensis A. Bor. with pesticide. J. Shenyang Agric Univ 25:264–267
  • Ming HQ (1986) The synthesis of salidroside and its pharmacological properties. Chin J Pharm Bull 21:373–375
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
  • Nguyen Hoang L, Nguyen Thi Duy N (2013) Production of asiaticoside from centella (Centella asiatica L. Urban) cells in bioreactor. Asian Pac J Trop Biomed 3:806–810
  • Paek KY, Hahn EJ, Son SH (2001) Application of bioreactors for large-scale micropropagation systems of plants. In Vitro Cell Dev Biol Plant 37:149–157
  • Palazon J, Mallol A, Eibl R, Lettenbauer C, Cusido RM, Pinol MT (2003) Growth and ginsenoside production in hairy root cultures of Panax ginseng using a novel bioreactor. Planta Med 69:344–349
  • Praveen N, Murthy HN (2012) Synthesis of withanolide A depends on carbon source and medium pH in hairy root cultures of Withania somnifera. Ind Crop Prod 35:241–243
  • Sakurai M, Mori T, Seki M, Furusaki S (1996) Changes of anthocyanin composition by conditioned medium and cell inoculum size using strawberry suspension culture. Biotechnol Lett 18:1149–1154
  • Scholten H, Pierik R (1998) Agar as a gelling agent: chemical and physical analysis. Plant Cell Rep 17:230–235
  • Shams AM, Hadjiakhoondi A, Jamshidi A, Forouzideh N, Tavakolizadeh M (2010) Production of secondary metabolites in intact plants and cultured cells of Thymus vulgaris L. Planta Med 76:1271
  • Sivakumar G, Paek K (2005) Methyl jasmonate induce enhanced production of soluble biophenols in Panax ginseng adventitious roots from commercial scale bioreactors. Chem Nat Compd 41:669–673
  • Sivakumar G, Kim SJ, Hahn EJ, Paek KY (2005) Optimizing environmental factors for large-scale multiplication of chrysanthemum (Chrysanthemum grandiflorum) in balloontype bioreactor culture In Vitro Cell. Dev Biol Plant 41: 822–825
  • Sivakumar G, Yu KW, Paek KY (2006) Enhanced production of bioactive ginsenosides from adventitious roots of Panax ginseng in bioreactor culture. J Hortic Sci Biotech 81:549–552
  • Sung IK, Han NS, Kim BS (2012) Co-production of biomass and metabolites by cell retention culture of Leuconostoc citreum. Bioproc Biosyst Eng 35:715–720
  • Wu YL (1988) The Exploration of the natural resources and protection of ecological environment in Chang Bai Mountain. Jilin Science Press, Changchun
  • Wu SX, Zu YG, Wu M (2003) High yield production of salidroside in the suspension culture of Rhodiola sachalinensis. J Biotechnol 106:33–43
  • Wu CH, Dewir YH, Hahn EJ, Paek KY (2006) Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of Echinacea angustifolia. J Plant Biol 49:193–199
  • Wu CH, Murthy HN, Hahn EJ, Paek KY (2007) Enhanced production of caftaric acid, chlorogenic acid and cichoric acid in suspension cultures of Echinacea purpurea by the manipulation of incubation temperature and photoperiod. Biochem Eng J 36:301–303
  • Xu JF, Zhao Y, Han AM, Meng PS (1995) Induction and culture of calli from Rhodiola sachalinesis A. Bor. Chin J Appl Environ Biol 1:19–25
  • Xu JF, Su ZG, Feng PS (1998a) Production of salidroside through biotransformation of exogenous tyrosol in Rhodiola sachalinensis cell suspension cultures. Acta Bot Sin 40:1129–1135
  • Xu JF, Su ZG, Feng PS (1998b) Suspension culture of compact callus aggregate of Rhodiola sachalinensis for improved salidroside production. Enzyme Microb Tech 23:20–27
  • Xu JF, Xie J, Han AM, Feng PS, Su ZG (1998c) Kinetic and technical studies on large-scale culture of Rhodiola sachalinensis compact callus aggregates with air-lift reactors. J Chem Technol Biotechnol 72:227–234
  • Xu JF, Ying PQ, Han AM, Su ZG (1999) Enhanced salidroside production in liquid-cultivated compact callus aggregates of Rhodiola sachalinensis: manipulation of plant growth regulators and sucrose. Plant Cell Tissue Organ Cult 55:53–58
  • Zhang YH, Zhong JJ (1997) Hyperproduction of ginseng saponin and polysaccharide by high density cultivation of Panax notoginseng cells. Enzyme Microb Technol 21:59–63
  • Zhao J, Davis L, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333

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