Quality of cooled semen of cold-blooded stallions evaluated with the use of apoptosis and DNA defragmentation markers

• Autorzy: Krakowski L. , Piech T. , Brodzki P. , Slawinska-Brych A. , Tatara M. R. , Bartoszek J.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to determine the quality of semen collected from stallions and chilled to +4°C. Semen was collected from eight Polish cold-blooded stallions, aged 4-8 years. The ejaculate was examined macro- and microscopically to evaluate volume, concentration, viable sperm percentage, progressive motility, and morphology. The percentage of live/dead spermatozoa was determined by eosin and nigrosin staining. Flow cytometry was applied to detect phosphatidylserine translocation, whereas DNA defragmentation was determined with a commercial kit. The semen samples were examined immediately after collection and dilution (time 0) and at 6 storage times: 24, 48, 72, 96, and 120 hours later. Time-related changes in all the investigated spermatozoa parameters were observed at 6 different times. Progressive motility was negatively correlated with the percentage of live spermatozoa with phosphatidylserine translocation, but positively correlated with DNA defragmentation (P < 0.05). This study demonstrates that the evaluation of selected indicators of apoptosis is very useful in assessing the quality of chilled stallion semen. The results obtained show what significant changes occur in spermatozoa during storage at +4°C. Evaluation of the cell membrane integrity and DNA defragmentation of spermatozoa makes it possible to determine whether and for how long semen chilled to +4°C may be used for artificial insemination, which is extremely important during semen transportation. The methods of semen quality evaluation applied in this study should also be used in determining the usefulness of chilled semen for artificial insemination. This may lead to higher fertilization rates in mares.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2014
• Tom: 70
• Numer: 09
• Opis fizyczny: p.572-577,ref.

Twórcy

autor

Krakowski L.

• Department of Andrology and Reproductive Biotechnology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, ul. Gleboka 30, 20-612 Lublin, Poland

autor

Piech T.

• Department of Andrology and Reproductive Biotechnology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, ul. Gleboka 30, 20-612 Lublin, Poland

autor

Brodzki P.

• Department of Andrology and Reproductive Biotechnology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, ul. Gleboka 30, 20-612 Lublin, Poland

autor

Slawinska-Brych A.

• Department of Cell Biology, Marie Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland

autor

Tatara M. R.

• Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, ul. Akademicka 12, 20-950 Lublin, Poland

autor

Bartoszek J.

• Department of Andrology and Reproductive Biotechnology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, ul. Gleboka 30, 20-612 Lublin, Poland

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