Isolation and characterization of human amniotic fluid and SH‑SY5Y/ BE(2)‑M17 cell derived exosomes

• Autorzy: Seyfizadeh N. , Seyfizadeh N. , Rahbarghazi R. , Nourazarian A. , Borzouisileh S. , Palideh A. , Elahimanesh F. , Hamishehkar H. , Salimi L. , Nouri M. , Abtin M.
• Języki publikacji: EN

Abstrakty

EN

The application of stem cells as a therapy for degenerative disease holds great promise. Substantial evidence suggests that stem cell derived exosomes are a novel cell‑free therapy for the corresponding cells. Exosomes are less complex as compared to their parental cells, due to the fewer number of membrane proteins. In addition, the smaller size and lower risk of immunogenicity makes exosomes potentially safe therapeutic nano‑carriers. A large number of ongoing research studies are focused on characterizing exosomes that were derived from different sources, for their potential use in various therapeutic applications. In the present study, we focused on characterizing human amniotic fluid stem cell derived exosomes for future therapeutic applications, such as paracrine therapy/nano carrier. In addition, we characterized exosomes derived from SH‑SY5Y and BE(2)‑M17 cells, which are a known neuronal model, for further characteristic analyses of neuronal differentiation and neurobiology. Finally, we compared various exosome isolation techniques and procedures and evaluated exosome yield.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2019
• Tom: 79
• Numer: 3
• Opis fizyczny: p.261-269,fig.,ref.

Twórcy

autor

Seyfizadeh N.

• Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
• Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

autor

Seyfizadeh N.

• National Center for Tumor Diseases, University of Heidelberg, Heidelberg, Germany

autor

Rahbarghazi R.

• Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

autor

Nourazarian A.

• Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

autor

Borzouisileh S.

• Medical Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran

autor

Palideh A.

• Faculty of Dentistry, Babol University of Medical Sciences, Babol, Iran

autor

Elahimanesh F.

• Department of Radiology, School of Paramedical Science, Kurdistan University of Medical Sciences, Sanandaj, Iran

autor

Hamishehkar H.

• Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

autor

Salimi L.

• Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

autor

Nouri M.

• Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
• Stem Cell and Regenerative Medicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran

autor

Abtin M.

• Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

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Identyfikatory

DOI

10.21307/ane‑2019‑024