The influence of maternal separation stress on LTP in the CA1 area of the rat hippocampus

• Autorzy: Trenk A. , Danielewicz J. , Hess G.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: The maternal separation (MS) of rat pups is a widely used paradigm to study the influence of early life adversities on the functions of the adult organism. MS stress exerts deleterious effects during the development of the brain. It has been shown that MS decreases the number of nerve cells in hippocampus and profoundly affects behavior of adult animals. However, the influence of MS on hippocampal synaptic plasticity is still poorly understood. Here we examined the effects of MS on long-term potentiation (LTP) in the CA1 area of the rat hippocampus. METHODS:Rat pups were subjected to MS (3 h/day) on postnatal days (PND) 1–21. They were weaned at PND 28 and between PND 42–60 the electrophysiological experiments were conducted. Coronal brain slices containing the hippocampus were incubated in ACSF containing either 1 mM or 0.1 mM Mg2+. Field excitatory postsynaptic potentials (fEPSP) were recorded from the CA1 region of the hippocampus and LTP was induced by high frequency stimulation (HFS). RESULTS: In the present study we observed an impairment of LTP in slices obtained from MS-subjected animals, compared to control, when the slices were incubated in ACSF containing a low concentration of Mg2+ ions. In contrast, in ACSF containing 1 mM Mg2+ the magnitude of LTP induced in control slices and in the slices isolated from MS rats was similar. CONCLUSION: The effect of MS on hippocampal LTP appears to depend on different conditions of the NMDA receptor activation. Support: National Science Center, Poland, grant no. 2011/03/N/ NZ4/02176.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S83

Twórcy

autor

Trenk A.

• Department of Neurophysiology and Chronobiology, Institute of Zoology, Jagiellonian University, Krakow, Poland

autor

Danielewicz J.

• Department of Neurophysiology and Chronobiology, Institute of Zoology, Jagiellonian University, Krakow, Poland

autor

Hess G.

• Department of Neurophysiology and Chronobiology, Institute of Zoology, Jagiellonian University, Krakow, Poland
• Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland