Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 35 | 05 |
Tytuł artykułu

Calcium-dependent protein kinase gene ZmCPK12 from maize confers tolerance to drought and salt stresses in transgenic plants

Warianty tytułu
Języki publikacji
Calcium-dependent protein kinases (CDPKs) are important calcium sensors in calcium-mediated signal transductions in plant cells. Increasing evidence shows that CDPKs play vital regulatory roles in abiotic stress responses in plants. We isolated the ZmCPK12 gene encoding a CDPK from maize seedlings. The predicted ZmCPK12 protein contains a typical conserved CDPK structure, a Ser/Thr kinase domain and four EF-hand domains. A ZmCPK12::hGFP fusion protein was localized in the cytoplasm and nucleus when introduced into Arabidopsis mesophyll protoplasts. ZmCPK12 transcription was highly activated by drought and salt in maize seedlings. Isolation of the ZmCPK12 promoter revealed some cis-acting elements that respond to stress. Overexpression of ZmCPK12 improved plant survival rates under drought conditions and enhanced chlorophyll content in transgenic Arabidopsis plants subjected to salt stress. This study revealed that ZmCPK12 is involved in response to environmental stresses, and that ZmCPK12 can be used for genetic improvement in tolerance to environmental stresses of plants.
Słowa kluczowe
Opis fizyczny
  • Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing 100037, People’s Republic of China
  • School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People’s Republic of China
  • Aono M, Kubo A, Saji H, Tanaka K, Kondo N (1993) Enhanced tolerance to photooxidative stress of transgenic Nicotiana tabacum with high chloroplastic glutathione reductase activity. Plant Cell Physiol 34:129–136
  • Bulgakov VP, Gorpenchenko TY, Shkryl YN, Veremeichik GN, Mischenko NP, Avramenko TV, Fedoreyev SA, Zhuravlev YN (2011) CDPK-driven changes in the intracellular ROS level and plant secondary metabolism. Bioeng Bugs 2:327–330
  • Choi HI, Park HJ, Park JH, Kim S, Im MY, Seo HH, Kim YW, Hwang I, Kim SY (2005) Arabidopsis calcium-dependent protein kinase AtCPK32 interacts with ABF4, a transcriptional regulator of abscisic acid-responsive gene expression, and modulates its activity. Plant Physiol 139:1750–1761
  • Hrabak EM, Chan CW, Gribskov M, Harper JF, Choi JH, Halford N, Kudla J, Luan S, Nimmo HG, Sussman MR, Thomas M, Walker-Simmons K, Zhu JK, Harmon AC (2003) The Arabidopsis CDPK-SnRK superfamily of protein kinases. Plant Physiol 132:666–680
  • Jain M, Pathak BP, Harmon AC, Tillman BL, Gallo M (2011) Calcium dependent protein kinase (CDPK) expression during fruit development in cultivated peanut (Arachis hypogaea) under Ca2+-sufficient and—deficient growth regimens. J Plant Physiol 168:2272–2277
  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-44CT method. Methods 25:402–408
  • Ludwig AA, Romeis T, Jones JD (2004) CDPK-mediated signaling pathways, specificity and cross-talk. J Exp Bot 55:181–188
  • Ma SY, Wu WH (2007) AtCPK23 functions in Arabidopsis responses to drought and salt stresses. Plant Mol Biol 65:511–518
  • Rutschmann F, Stalder U, Piotrowski M, Oecking C, Schaller A (2002) LeCPK1, a calcium-dependent protein kinase from tomato. Plasma membrane targeting and biochemical characterization. Plant Physiol 129:156–168
  • Saijo Y, Hata S, Kyozuka J, Shimamoto K, Izui K (2000) Overexpression of a single Ca2=-dependent protein kinase confers both cold and salt/drought tolerance on rice plants. Plant J 23:319–327
  • Saijo Y, Kinoshita N, Ishiyama K, Hata S, Kyozuka J, Hayakawa T, Nakamura T, Shimamoto K, Yamaya T, Izui K (2001) A Ca(2+)-dependent protein kinase that endows rice plants with cold- and salt-stress tolerance functions in vascular bundles. Plant Cell Physiol 42:1228–1233
  • Szczegielniak J, Borkiewicz L, Szurmak B, Lewandowska-Gnatowska E, Statkiewicz M, Klimecka M, Cieśla J, Muszyńska G (2012) Maize calcium-dependent protein kinase (ZmCPK11): local and systemic response to wounding, regulation by touch and components of jasmonate signaling. Physiol Plant 146:1–14
  • Wan B, Lin Y, Mou T (2007) Expression of rice Ca(2+)-dependent protein kinases (CDPKs) genes under different environmental stresses. FEBS Lett 581:1179–1189
  • Xu ZS, Ma YZ, Cheng XG, Cao LX, Li LC, Chen M (2006) Isolation and characterization of GmSTY1, a novel gene encoding a dualspecificity protein pinase in soybean (Glycine max L.). J Integr Plant Biol 48:857–866
  • Xu ZS, Xia LQ, Chen M, Cheng XG, Zhang RY, Li LC, Zhao YX, Lu Y, Ni ZY, Liu L, Qiu ZG, Ma YZ (2007) Isolation and molecular characterization of the Triticum aestivum L. ethyleneresponsive factor 1 (TaERF1) that increases multiple stress tolerance. Plant Mol Biol 65:719–732
  • Xu ZS, Chen M, Li LC, Ma YZ (2008a) Functions of the ERF transcription factor family in plants. Botany 86:969–977
  • Xu ZS, Ni ZY, Liu L, Nie LN, Li LC, Chen M, Ma YZ (2008b) Characterization of the TaAIDFa gene encoding a CRT/DREbinding factor responsive to drought, high-salt, and cold stress in wheat. Mol Genet Genomics 280:497–508
  • Xu ZS, Liu L, Ni ZY, Liu P, Chen M, Li LC, Chen YF, Ma YZ (2009a) W55a encodes a novel protein kinase that is involved in multiple stress responses. J Integr Plant Biol 51:58–66
  • Xu ZS, Xiong TF, Ni ZY, Chen XP, Chen M, Li LC, Gao DY, Yu XD, Liu P, Ma YZ (2009b) Isolation and identification of two genes encoding leucine-rich repeat (LRR) proteins differentially responsive to pathogen attack and salt stress in tobacco. Plant Sci 176:38–45
  • Xu ZS, Chen M, Li LC, Ma YZ (2011) Functions and application of the AP2/ERF transcription factor family in crop improvement. J Integr Plant Biol 53:570–585
  • Yoo SD, Cho YH, Sheen J (2007) Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc 2:1565–1572
  • Zhao R, Sun HL, Mei C, Wang XJ, Yan L, Liu R, Zhang XF, Wang XF, Zhang DP (2011a) The Arabidopsis Ca2+-dependent protein kinase CPK12 negatively regulates abscisic acid signaling in seed germination and post-germination growth. New Phytol 192:61–73
  • Zhao R, Wang XF, Zhang DP (2011b) CPK12: a Ca2+-dependent protein kinase balancer in abscisic acid signaling. Plant Signal Behav 6:1687–1690
  • Zheng WJ, Xu ZS, Chen M, Li LC, Chai SC, Ma YZ (2012) Isolation and characterization of receptor-like protein kinase WELP1 in wheat. Afr J Microbiol Res 6:2410–2418
  • Zhu SY, Yu XC, Wang XJ, Zhao R, Li Y, Fan RC, Shang Y, Du SY, Wang XF, Wu FQ, Xu YH, Zhang XY, Zhang DP (2007) Two calcium-dependent protein kinases, CPK4 and CPK11, regulate abscisic acid signal transduction in Arabidopsis. Plant Cell 19:3019–3036
  • Zou JJ, Wei FJ, Wang C, Wu JJ, Ratnasekera D, Liu WX, Wu WH (2010) Arabidopsis calcium-dependent protein kinase CPK10 functions in abscisic acid- and Ca2+-mediated stomatal regulation in response to drought stress. Plant Physiol 154:1232–1243
rekord w opracowaniu
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.