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2013 | 58 | 4 |
Tytuł artykułu

Fast and efficient DNA-based method for winter diet analysis from stools of three cervids: moose, red deer, and roe deer

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Effects of cervid browsing on timber production, especially during winter, lead to economic losses in forest management. The aim of this study was to present an efficient DNA-based method which allows qualitative assessment of the winter diet from stools of moose (Alces alces), roe deer (Capreolus capreolus), and red deer (Cervus elaphus). The preliminary results of the diet composition of the three cervids from Poland were also presented with a special emphasis on moose. The electropherograms of the chloroplast intron trnL (UAA) P6 loop amplification products using g (fluorescence-labeled) and h primers revealed differences in the length of PCR products among various plant species eaten by these herbivores. In addition, the usage of species-specific primers allowed unambiguous identification of different gymnosperms and angiosperms. The preliminary moose diet analysis, based on winter fecal samples from the entire range of moose occurrence in Poland, revealed the presence of 15 to 24 tree, shrub, and herbaceous species. This fast, cost-efficient, and simple method proved also to be reliable for the diet analysis of red deer and roe deer. It may be a valuable tool in forest and conservation management, as well as a way of enhancing ecological studies focusing on the impact of herbivores on the ecosystems and their possible food niche overlap.
Słowa kluczowe
EN
Wydawca
-
Czasopismo
Rocznik
Tom
58
Numer
4
Opis fizyczny
p.379-386,fig.,ref.
Twórcy
autor
  • Institute of Biology, University of Bialystok, Swierkowa 20B, 15-950 Bialystok, Poland
autor
  • Laboratoire d'Ecologie Alpine, CNRS, UMR 5553, Universite Joseph Fourier, 2233 Rue de la Piscine, 38041 Grenoble Cedex 9, France
autor
  • Institute of Biology, University of Bialystok, Swierkowa 20B, 15-950 Bialystok, Poland
  • Institute of Biology, University of Bialystok, Swierkowa 20B, 15-950 Bialystok, Poland
autor
  • Institute of Biology, University of Bialystok, Swierkowa 20B, 15-950 Bialystok, Poland
  • Institute of Biology, University of Bialystok, Swierkowa 20B, 15-950 Bialystok, Poland
Bibliografia
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  • Dzięciołowski R (1974) Selection of browse twigs by moose. Acta Theriol 19:273–281
  • Dzięciołowski R, Pielowski Z (1975) Łoś. Państwowe Wydawnictwo Rolnicze i Leśne, Warsaw
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  • Gębczyńska Z, Raczyński J (1989) Distribution, population structure, and social organization of moose in the Biebrza Valley, Poland. Acta Theriol 34:195–217
  • Gębczyńska Z, Raczyński J (1993) Problems of protection and management of the moose and other game in the Biebrza National Park. Park Nar Rezerw Przyr 12:5–36 [In Polish with English summary]
  • Graham RT, Jain TB, Kingery JL (2010) Ameliorating conflicts among deer, elk, cattle and/or other ungulates and other forest uses: a synthesis. Forestry 83:245–255. doi:10.​1093/​forestry/​cpq003
  • Hofmann RR (1989) Evolutionary steps of ecophysiological adaptation and diversification of ruminants: a comparative view of their digestive system. Oecologia 78:443–457
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  • Komenda E (2001) Wpływ wzbogacania bazy żerowej na populację łosi i drzewostany Doliny Biebrzy. Dissertation, Warsaw University of Life Sciences - SGGW
  • Kowalczyk R, Taberlet P, Coissac E, Valentini A, Miquel C, Kamiński T, Wójcik JM (2011) Influence of management practices on large herbivore diet—case of European bison in Białowieża Primeval Forest (Poland). For Ecol Manag 261:821–828. doi:10.​1016/​j.​foreco.​2010.​11.​026
  • Morow K (1976) Food habits of moose from Augustów Forest. Acta Theriol 21:101–116
  • Pompanon F, Deagle BE, Symondson WOC, Brown DS, Jarman SN, Taberlet P (2012) Who is eating what: diet assessment using next generation sequencing. Mol Ecol 21:1931–1950. doi:10.​1111/​j.​1365-294X.​2011.​05403.​x
  • Putman RJ (1996) Ungulates in temperate forest ecosystems: perspectives and recommendations for future research. For Ecol Manag 88:205–214
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  • Świsłocka M, Ratkiewicz M, Borkowska A, Komenda E, Raczyński J (2008) Mitochondrial DNA diversity in the moose, Alces alces, from northeastern Poland: evidence for admixture in a bottlenecked relict population in the Biebrza valley. Ann Zool Fenn 45:360–365
  • Taberlet P, Coissac E, Pompanon F, Gielly L, Miquel C, Valentini A, Vermat T, Corthier G, Brochmann C, Willerslev E (2007) Power and limitations of the chloroplast trnL (UAA) intron for plant DNA barcoding. Nucl Acids Res 35:1–8. doi:10.​1093/​nar/​gkl938
  • Valentini A, Miquel C, Nawaz MA, Bellemain E, Coissac E, Pompanon F, Gielly L, Cruaud C, Nascetti G, Wincker P, Swenson JE, Taberlet P (2009) New perspectives in diet analysis based on DNA barcoding and parallel pyrosequencing: the trnL approach. Mol Ecol Resour 9:51–60. doi:10.​1111/​j.​1755-0998.​2008.​02352.​x
  • Wam HK, Hjeljord O (2010) Moose summer and winter diets along a large scale gradient of forage availability in southern Norway. Eur J Wildl Res 56:745–755. doi:10.​1007/​s10344-010-0370-4
Typ dokumentu
Bibliografia
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Identyfikator YADDA
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