Diversity of gag gene sequence encoding immunodominant epitope on capsid protein of lentiviruses from sheep in Poland

• Autorzy: Olech M , Rola-Luszczak M. , Kozaczynska B. , Kubis P. , Pluta A. , Gil A. , Kuzmak J.
• Języki publikacji: EN

Abstrakty

EN

In the study, a 122 bp fragment of gag gene encoding immunodominant epitope on capsid protein of small ruminant lentiviruses (SRLVs) found in sheep was amplified by PCR and analysed by SSCP and sequencing. Out of 30 DNA samples, five showed different migration patterns, demonstrating the individual variations within gag sequences, which were confirmed afterwards by sequence analysis. In two samples nucleotide changes yielded amino acid substitutions highlighting the conservative nature of gag encoded immunoreactive epitope but also potencial insensitivity of a single-strain-based immunoassay.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2012
• Tom: 56
• Numer: 4
• Opis fizyczny: p.431-434,fig.,ref.

Twórcy

autor

Olech M

• Department of Biochemistry, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Rola-Luszczak M.

• Department of Biochemistry, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kozaczynska B.

• Department of Biochemistry, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kubis P.

• Department of Biochemistry, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Pluta A.

• Department of Biochemistry, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Gil A.

• Department of Biochemistry, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kuzmak J.

• Department of Biochemistry, National Veterinary Research Institute, 24-100 Pulawy, Poland

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