A method for the infectivity discrimination of enveloped DNA viruses using palladium compounds pre-treatment followed by real-time PCR

• Autorzy: Krzyzankova M. , Krasna M. , Prodelalova J. , Vasickova P.
• Języki publikacji: EN

Abstrakty

EN

Cultivation-based assays represent the gold standard for the assessment of virus infectivity; however, they are time-consuming and not suitable for every virus type. Pre-treatment with platinum (Pt) compounds followed by real-time PCR has been shown to discriminate between infectious and non-infectious RNA viruses. This study examined the effect of Pt and palladium (Pd) compounds on enveloped DNA viruses, paying attention to two significant pathogens of livestock – bovine herpesvirus-1 (BoHV-1) and African swine fever virus (ASFV). Native or heat-treated BoHV-1 suspension was incubated with the spectrum of Pt/Pd compounds. Bis(benzonitrile)palladium(II) dichloride (BB-PdCl2) and dichloro(1,5-cyclooctadiene) palladium(II) (PdCl2-COD) produced the highest differences found between native and heat-treated viruses. Optimized pre-treatment conditions (1 mM of Pd compound, 15 min, 4°C) were applied on both virus genera and the heat inactivation profiles were assessed. A significant decrease in the detected quantity of BoHV-1 DNA and ASFV DNA after heat-treatment (60°C and 95°C) and consequent incubation with Pd compounds was observed. BB-PdCl2 and PdCl2-COD could help to distinguish between infectious and non-infectious enveloped DNA viruses such as BoHV-1 or ASFV.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2023
• Tom: 26
• Numer: 2
• Opis fizyczny: p.211-221,fig.,ref.

Twórcy

autor

Krzyzankova M.

• Department of Microbiology and Antimicrobial Resistance, Food and Environmental Virology, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic

autor

Krasna M.

• Department of Microbiology and Antimicrobial Resistance, Food and Environmental Virology, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic

autor

Prodelalova J.

• Department of Infectious Diseases and Preventive Medicine, Molecular Epidemiology of Viral Infections, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic

autor

Vasickova P.

• Department of Microbiology and Antimicrobial Resistance, Food and Environmental Virology, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic

Identyfikatory

DOI

10.24425/pjvs.2023.145024