The effect of seed conditioning, short-term heat shock and salicylic, jasmonic acid or brasinolide on sunflower (Helianthus annuus L.) chilling resistance and polysome formation

• Autorzy: Gornik K. , Badowiec A. , Weidner S.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to develop the method for increasing resistance of sunflower seedlings ‘Wielkopolski’ to chilling. Seeds were conditioned at 25°C for 2 days in water to 15, 20 and 25 % moisture content or in salicylic or jasmonic acid in concentration of 10⁻² ; 10⁻³ and 10⁻⁴ M or brassinolide in concentration of 10⁻⁶ ; 10⁻⁸ and 10⁻¹⁰–15 % moisture content. After 2 days of incubation the conditioned seeds were heat shocked at 45°C for 0, 30, 60, 120 and 240 min and 5 mm seedlings were exposed to chilling at 0°C for 21 days. The effectiveness of the methods was assessed by evaluation of roots growth in Phytotoxkit Microbiotest, changes in the activity of dehydrogenases, the integrity of the cytoplasmic membrane and formation of polysomes after seedling were returned to 25°C for 72 h. Seeds were conditioned at 25°C for 2 days in water to 15 % moisture content and then heat shocked at 45°C for 2 h decreased chilling injury of seedlings expressed by subsequent growth of the roots, electrolyte leakage, dehydrogenases activity and polysomes formation. Application of heat shock of 45°C for 2 h during seed conditioning additionally provided seedling protection against subsequent chilling conditions. Brasinolide, salicylic acid or jasmonic acid applied during seeds conditioning exhibited further beneficial effect on seedling resistance to chilling. The most pronounced effect was obtained due to seed conditioning to 15 % moisture content in solutions of brassinolide in concentration of 10⁻⁸ M. After 2 days of imbibition treated in this way seeds were exposed to heat shock at 45°C for 2 h. The role of physiological events in improvement of sunflower chilling tolerance are discussed.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 10
• Opis fizyczny: p.2547-2554,fig.,ref.

Twórcy

autor

Gornik K.

• Departament of Ornamental Nursery and Seed Science, Research Institute of Horticulture, Konstytucji 3 Maja 1/3, Skierniewice, Poland

autor

Badowiec A.

• Department of Biochemistry Faculty of Biology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

autor

Weidner S.

• Department of Biochemistry Faculty of Biology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

Bibliografia

• Abe S, Ito Y, Davies E (1992) Co-sedimentation of actin, tubulin and membranes in the cytoskeleton fractions from peas and mouse 3T3 cells. J Exp Bot 43:941–949
• Bajguz A (2010) An enhancing effect of exogenous brassinolide on the growth and antioxidant activity in Chlorella vulgaris cultures under heavy metals stress. Environ Exp Bot 68:175–179
• Bajguz A, Czerpak R (1998) Physiological and biochemical role of brassinosteroids and their structure-activity relationship in the green alga Chlorella vulgaris Beijerinck (Chlorophyceae). J Plant Growth Regul 17:131–139
• Bajguz A, Hayat S (2009) Effects of brassinosteroids on the plant responses to environmental stresses. Plant Physiol Biochem 47:1–8
• Białecka B, Kępczyński J (2010) Germination, α-, β-amylase and total dehydrogenase activities of Amaranthus caudatus seeds under water stress In the presence of ethephon or gibberellin A₃. Acta Biol Cracov Ser Bot 52:7–12
• Bradlow J (1990) Chilling sensitivity of photosynthetic oil-seedlings. I cotton and sunflower. J Exp Bot 41:1585–1593
• Bramlage WJ, Leopold AC, Parrish DT (1978) Chilling stress to soybeans during imbibition. Plant Physiol 61:525–529
• Branco-Price C, Kaiser A, Jang CJH, Larive CK, Bailey-Serres J (2008) Selective mRNA translation coordinates energetic and metabolic adjustments to cellular oxygen deprivation and reoxygenation in Arabidopsis thaliana. Plant J 56:743–755
• Collins GG, Nie X, Saltveit ME (1993) Heat-shock increases chilling tolerance of mung bean hypocotyl tissue. Physiol Plant 89:117–124
• Davies E, Abe S (1995) Methods for isolation and analysis of polyribosomes. In: Galbraith DW, Bourque DP, Bohnert HJ (eds) Methods in cell biology. Methods in plant cell biology. Academic Press, New York, pp 209–222
• Deef HE (2007) Influence of salicylic acid on stress tolerance during seed germination of Triticum aestivum and Hordeum vulgare. Adv Biol Res 1(1–2):40–48
• Dhaubhadel S, Chaudhary S, Dobinson KF, Krishna P (1999) Treatment with 24-epibrassinolide, a brassinosteroid, increases the basic thermotolerance of Brassica napus and tomato seedlings. Plant Mol Biol 40:333–342
• Dhaubhadel S, Browning KS, Gallie DR, Krishna P (2002) Brassinosteroid functions to protect the translational machinery and heat-shock protein synthesis following thermal stress. Plant J 29:681–691
• Fariduddin Q, Hayat S, Ali B, Ahmad A (2005) Effect of 28-homobrassinolide on the growth and yield of Vigna radiata. Ann Biol 21:1–6
• Farooq M, Basra SMA, Rehman H, Saleem BA (2008) Seed priming enhances the performance of late sown wheat (Triticum aestivum L.) by improving chilling tolerance. J Agron Crop Sci 194:55–60
• Farooq M, Wahid A, Ahmad N, Asad SA (2010) Comparative efficacy of surface drying and re-drying seed priming in rice: changes in emergence, seedling growth and associated metabolic events. Paddy Water Environ 8:15–22
• Gharib FA, Hegazi AZ (2010) Salicylic acid ameliorates germination, seedling growth, phytohormone and enzymes activity in bean (Phaseolus vulgaris L.) under cold stress. J Am Sci 6:675–683
• Górnik K (2011) The effect of temperature treatments during ‘Wielkopolski’ sunflower seed imbibition and storage on plant tolerance to chilling. Folia Hortic 23:83–88
• Górnik K, Grzesik M (2002) Effect of Asahi SL on China aster ‘‘Aleksandra’’ sees yield, germination and same metabolic events. Acta Physiol Plant 24:379–383
• Gualerzi C, Cammarano P (1969) Comparative electrophoretic studies on the protein of chloroplast and cytoplasmic ribosomes of spinach leaves. Biochim Biophys Acta 190:170–186
• Hayat S, Ahmad A (2003) Soaking-seeds of Lens culinaris with 28-homobrassinolide increased nitrate reductase activity and grain yield in the field in India. Ann Appl Biol 143:121–124
• He RY, Wang GJ, Wang XS (1991) Effect of brassinolide on growth and chilling resistance of maize seedlings. In: Cutler HG, Yokota T, Adam G (eds) Brassinosteroids: chemistry, bioactivity and applications. American Chemical Society, Washington, pp 220–230
• Janda T, Szalai G, Tari I, Paldi E (1999) Hydroponic treatment with salicylic acid decreases the effects of Schilling injury in maize (Zea mays L.) plants. Planta 208:175–180
• Jennings P, Saltveit ME (1994) Temperature effects on imbibition and germination of cucumber (Cucumis sativus) seeds. J Am Soc Hortic Sci 119:464–467
• Kang H, Saltveit ME (2001) Activity of enzymatic antioxidant defense systems in chilled and heat shocked cucumber seedling radicals. Physiol Plant 113:548–556
• Kasai K, Mori N, Nakamura C (1998) Changes in the respiratory pathways during germination and early seedling growth of common wheat under normal and NaCl stressed conditions. Cereal Res Comm 26:217–224
• Kawaguchi R, Girke T, Bray EA, Bailey-Serres J (2004) Differential mRNA translation contributes to gene regulation under nonstress and dehydration stress conditions in Arabidopsis thaliana. Plant J 38:823–839
• Khripach VA, Zhabinskii VN, Groot AE (1999) Brassinosteroids: a new class of plant hormones. Academic Press, San Diego Kluza-Wieloch M (2003) Plant height at different developmental stages in observed types of sunflower (Hellianthus annus L.) cultivars. Rocz AR Pozn 6:93–105
• Korkmaz A, Ozbay N, Tiryaki I, Nas MN (2005) Combining priming and plant growth regulators improves muskmelon germination and emergence at low temperatures. Eur J Hortic Sci 70:29–34
• Kosowska M, Frączek E, Amarowicz R, Karamać M, Abe S, Weidner S (2004) Water-deficit-induced changes in cytoskeleton-bound and other polysomal populations in embryonic tissue during triticale caryopsis germination. Acta Physiol Plant 26:67–74
• Mangrich ME, Saltveit ME (2000) Effect of chilling, heat shock, and vigour on the growth of cucumber (Cucumis sativus) radicles. Physiol Plant 109:137–142
• Noll H (2008) The discovery of polyribosomes. BioEssays 30:1220–1234
• Pandey DK (1989) Ageing of French bean seeds at ambient temperature in relation to vigour and viability. Seed Sci Technol 17:41–47
• Persoone G, Vangheluwe ML (2000) Toxicity determination of the sediments of the river Seine in France by application of a battery of microbiotests. In: Persoone G, Janssen C, De Coen W (eds) New Microbiotests for Routine Toxicity Screening and Biomonitoring. Kluwer Academic, New York, pp 427–439
• Posmyk M, Corbineau F, Vinel D, Come D (1999) Effects of priming on physiological and metabolic events induced by chilling in soybean (Glycine max (L.) Merr.) seeds. Zeszyty Problemowe Postępów Nauk Rolniczych 469:117–125
• Posmyk MM, Corbineau F, Vinel D, Bailly C, Come D (2001) Osmoconditioning reduces physiological and biochemical damage induced by chilling in soybean seeds. Physiol Plant 111:473–482
• Rajasekaran LR, Stiles A, Caldwell CD (2002) Stand establishment in processing carrot-effects of various temperature regimes on germination and the role of salicylates in promoting germination at low temperatures. Can J Plant Sci 82:443–450
• Saltveit ME (1989) A kinetic examination of iron leakage from chilled tomato pericarp discs. Acta Hortic 258:617–622
• Saltveit ME (2001) Chilling injury is reduced in cucumber and rice seedlings and in tomato pericarp discs by heat-shocks applied after chilling. Postharvest Biol Technol 21:169–177
• Shakirowa FM, Sakhabutdinova AR, Bezrukova MV, Fatkhutdinova RA, Fatkhutdinova DR (2003) Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Sci 164:317–322
• Swaminathan S, Masek T, Molin C, Pospisek M, Sunnerhagen P (2006) Rck2 is required for reprogramming of ribosomes during oxidative stress. Mol Biol Cell 17:1472–1482
• Yángüez E, Castro-San AB, Fernandez-Bautista N, Oliveros JC, Castellano MM (2013) Analysis of genome-wide changes in the translatome of Arabidopsis seedlings subjected to heat stress. PLoS One 8:e71425

Identyfikatory

DOI

10.1007/s11738-014-1626-5