Immunohistochemical evaluation of apoptosis and proliferation in the mucous membrane of selected uterine regions in pre - pubertal bitches exposed to low doses of zearalenone

• Autorzy: Stopa E. , Babinska I. , Zielonka L. , Gajecki M. , Gajecka M.
• Języki publikacji: EN

Abstrakty

EN

Mycotoxins are toxic secondary metabolites produced by fungi. Those biologically active compounds occur naturally and they include zearalenone (ZEN), a mycotoxin that contaminates plant material, including the ingredients used in the production of commercial dog food. The influence of monotonic, low-dose and long-term exposure to ZEN on pre-pubertal bitches has not been fully explored to date. This paper describes a 42-day experiment performed on clinically healthy female dogs aged approximately 70 days, with estimated body weight of 8 kg. The animals were randomly divided into two experimental groups (EI and EII) and a control group (C) of 10 animals each. Group EI received 50 μg ZEN/kg (of body weight) per os, group EII received 75 μg ZEN/kg BW per os, and the control group was administered placebo. The bitches were ovariohysterectomized at the end of the experiment (at around 112 days of age), and selected sections of the uterine wall were subjected to immunohistochemical analyses (TUNEL and PCNA). A shift towards higher apoptotic (AI) and proliferative index (PI) was observed, in particular in group EI. Higher AI and PI values were noted in the epithelium of all uterine regions analysed and in the uterine glands in the uterine horn proximal to the body of the uterus.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2016
• Tom: 19
• Numer: 1
• Opis fizyczny: p.175-186,fig.,ref.

Twórcy

autor

Stopa E.

• Veterinary Clinic, Dabrowskiego 15, 14-200 Ilawa, Poland

autor

Babinska I.

• Department of Pathophysiology, Forensic Veterinary Medicine and Administration, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13-14, 10-718 Olsztyn, Poland

autor

Zielonka L.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13/29, 10-718 Olsztyn, Poland

autor

Gajecki M.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13/29, 10-718 Olsztyn, Poland

autor

Gajecka M.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13/29, 10-718 Olsztyn, Poland
• Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13/01, 10-718 Olsztyn, Poland

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Identyfikatory

DOI

10.1515/pjvs-2016-0022