A simple method for the determination of the cholesterol esterase activity

• Autorzy: Stepien A. E. , Gonchar M.
• Języki publikacji: EN

Abstrakty

EN

The proposed method determines the activity of cholesterol esterase (CEH) and takes advantage of its ability to catalyze the hydrolysis of cholesterol esters naturally present in human serum. The assay is based on Allain's method of spectrophotometric determination of cholesterol by means of cholesterol oxidase, peroxidase, but using 3,5-dichloro-dihydroxybenzenesulfonic acid (DHBS) as phenolic chromogen and human serum as a source of substrate for the CEH as a novelty. Furthermore, it is characterized by low costs and high precision. It can be employed to control the activity of CE preparations used for the preparation of enzymatic kits for the determination of cholesterol or for screening of potential bacterial enzyme producers.

Słowa kluczowe

EN

simple method; determination; activity determination; cholesterol esterase; esterase activity; human serum

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2013
• Tom: 60
• Numer: 3
• Opis fizyczny: p.401-403,fig.,ref.

Twórcy

autor

Stepien A. E.

• Department of Biotechnology, Institute of Applied Biotechnology and Basic Science, University of Rzeszow, Kolbuszowa, Poland
• Centre of Applied Biotechnology and Basic Sciences, University of Rzeszow, Kolbuszowa, Poland

autor

Gonchar M.

• Department of Biotechnology, Institute of Applied Biotechnology and Basic Science, University of Rzeszow, Kolbuszowa, Poland
• Centre of Applied Biotechnology and Basic Sciences, University of Rzeszow, Kolbuszowa, Poland
• Department of Analytical Biotechnology, Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine

Bibliografia

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