Oddziaływanie przemysłu miedziowego na kształtowanie się wybranych metali ciężkich i wskaźników biochemicznych we krwi krów mlecznych

• Autorzy: Kolacz R. , Dobrzanski Z. , Kupczynski R. , Cwynar P. , Opalinski S. , Pogoda-Sewerniak K.

Warianty tytułu

EN

Impact of the copper industry on the content of selected heavy metals and biochemical indicators in the blood of dairy cows

• Języki publikacji: PL

Abstrakty

EN

The aim of the study was to compare the levels of selected blood parameters of dairy cattle in an area with copper industry (LGOM, area I, n = 21) and the Wroclaw agricultural suburban area (area II, n = 20). The concentration of heavy metals Cd, Cu, Pb and Zn (atomic absorption spectrometry method) and Hg (spectrometric method) was analysed in full blood. However, other parameters were determined in the blood serum: Ca, P (inorganic), Mg and Fe (photometric method), aspartate aminotransferase (AST) – an enzyme test in accordance with the recommendations of the IFCC, lactate dehydrogenase (LDH) – the test optimized according to the guidelines DGKC, gamma-glutamyl transferase (GGT) – kinetic photometric test. Biochemical parameters in blood serum were determined with the use of an automatic biochemical analyser type Pentra 400 and reagents of Horiba ABX (France). Furthermore, the analysis covered: the total antioxidant capacity (TAS) – colorimetric assay based on the ABTS and peroxidase reaction as well as the activity of glutathione reductase (GR) – colorimetric test based on the reduction of glutathione (GSSG) in the presence of NADPH. These parameters were determined with the use of the same analyser and commercial assays kit of Randox company. The Cd concentration in both areas was very low and did not exceed 1.65 µg/l, while the Pb concentration in the LGOM region was 0.021 mg/l and 0.031 mg/l in area II. The level of mercury in area I in both regions was similar (about 0.03 µg/l). The copper content was significantly higher in area I than in region II (1.67 mgl/l and 0.72 mg/l respectively), which indicates the possibility of industry impact on copper accumulation in cows. The Zn concentration was significantly lower in the LGOM region than in area II (2.50 mg/l) in contrast to Cu. The AST activity was significantly lower in area I (66.56 U/l) than in area II (75.49 U/l). Similar findings were reported with GGT activity (lower concentration in area I – 21.42 U/l). The LDH levels in animals were similar in both areas (2015.0 and 1976.2 U/l respectively), as were TAS (1.12 and 1.16 mmol/l) and GR (422.4 and 429.3 U/l). Mean activity of AST and GGT in cows were present in both areas, reaching ranges of the reference values, but the activity of LDH was significantly higher. There are no reference values for TAS and GR. Similar concentrations of Ca, Mg and Fe were noticed in both regions during the study, while the content of P was significantly higher in area I. The mean concentration of detected macroelements were in the reference ranges. The analysis proved that the cooper industry does not negatively affect the content of biochemical parameters in the blood of dairy cows.

Słowa kluczowe

PL

Polska; przemysl miedziowy; Legnicko-Glogowski Okreg Miedziowy; zanieczyszczenia srodowiska; chow zwierzat; bydlo; krowy mleczne; badania krwi; wskazniki biochemiczne; metale ciezkie; kadm; miedz; rtec; olow; cynk; stezenie metali ciezkich

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2017
• Tom: 73
• Numer: 03
• Opis fizyczny: s.171-175,tab.,bibliogr.

Twórcy

autor

Kolacz R.

• Katedra Higieny Środowiska i Dobrostanu Zwierząt, Wydział Biologii i Hodowli Zwierząt, Uniwersytet Przyrodniczy we Wrocławiu, ul. Chełmońskiego 38C, 51-630 Wrocław

autor

Dobrzanski Z.

• Katedra Higieny Środowiska i Dobrostanu Zwierząt, Wydział Biologii i Hodowli Zwierząt, Uniwersytet Przyrodniczy we Wrocławiu, ul. Chełmońskiego 38C, 51-630 Wrocław

autor

Kupczynski R.

• Katedra Higieny Środowiska i Dobrostanu Zwierząt, Wydział Biologii i Hodowli Zwierząt, Uniwersytet Przyrodniczy we Wrocławiu, ul. Chełmońskiego 38C, 51-630 Wrocław

autor

Cwynar P.

• Katedra Higieny Środowiska i Dobrostanu Zwierząt, Wydział Biologii i Hodowli Zwierząt, Uniwersytet Przyrodniczy we Wrocławiu, ul. Chełmońskiego 38C, 51-630 Wrocław

autor

Opalinski S.

• Katedra Higieny Środowiska i Dobrostanu Zwierząt, Wydział Biologii i Hodowli Zwierząt, Uniwersytet Przyrodniczy we Wrocławiu, ul. Chełmońskiego 38C, 51-630 Wrocław

autor

Pogoda-Sewerniak K.

• Katedra Higieny Środowiska i Dobrostanu Zwierząt, Wydział Biologii i Hodowli Zwierząt, Uniwersytet Przyrodniczy we Wrocławiu, ul. Chełmońskiego 38C, 51-630 Wrocław

Bibliografia

• Akalın P. P., Ataseven V. S., Fırat D., Ergün Y., Başpınar N., Özcan O.: Selected biochemical and oxidative stress parameters and ceruloplasmin as acute phase protein associated with bovine leukaemia virus infection in dairy cows. Bull. Vet. Inst. Pulawy 2015, 59, 327-330.
• Alonso M. L., Benedito J. L., Miranda M., Castillo C., Hernandez J., Shore K. F.: Toxic and trace elements in liver, kidney and meat from cattle slaughtered in Galicia (NW Spain). Food Addit. Contam. 2000, 17, 447-457.
• Alonso M. L., Benedito J. L., Miranda M., Castillo C., Hernández J., Shore R. F.: Mercury concentrations in cattle from NW Spain. Sci. Total Environ. 2003, 302, 93-100.
• Barej R., Kwaśnicki R., Chojnacka K., Bolanowski J., Dobrzański Z., Pokorny P.: Mercury Content in Rural and Industrial Regions in Lower Silesia. Pol. J. Environ. Stud. 2009,18, 547-551.
• Bernabucci U., Ronchi B., Lacetera N., Nardone A.: Influence of body condition score on relationship between metabolic status and oxidative stress in periparturient dairy cows. J. Dairy Sci. 2005, 88, 2017-2026.
• Bortey-Sam N., Nakayama S. M. M., Ikenaka Y., Akoto O., Baidoo E., Beyene Y., Mizukawa H., Ishizuka M.: Human health risks from metals and metalloid via consumption of food animals near gold mines in Tarkwa, Ghana: Estimation of the daily intakes and target hazard quotients (THQs). Ecol. Environ. Safety 2015, 111, 160-167.
• Castillo C., Hernández J., Valverde I., Pereira V., Sotillo J., Alonso M. L., Benedito J. L.: Plasma malonaldehyde (MDA) and total antioxidant status (TAS) during lactation in dairy cows. Res. Vet. Sci. 2006, 80, 133-139.
• Center S. A.: Interpretation of liver enzymes. Vet. Clin. Small Anim. 2007, 37, 297-333.
• Checkley S., Waldner C. H., Blakley B.: Lead poisoning in cattle: Implications for food safety. Large Animal Vet. Rounds, 2002, vol. 2, iss. 8 (http://www.dairyweb. ca/Resources/WCVM/LAVR1002.pdf).
• Culotta V.: Superoxide dismutase, oxidative stress, and cell metabolism. Curr. Top. Cell. Regul. 2001, 36, 117-132.
• Cwynar P., Kołacz R., Grudnik T.: The Influence of Chronic Thallium Intoxication on Laying Hens, Including Its Cumulation in Tissues, Organs and Eggs. Pol. J. Environ. Stud. 2014, 23, 949-954.
• Czaban S., Kołacz R., Dobrzański Z., Bubel F., Opaliński S., Durkalec M., Gruszczyński M. F.: Zawartość rtęci w środowisku w rejonie oddziaływania obiektu unieszkodliwiania odpadów wydobywczych „Żelazny Most”. Przem. Chem. 2013, 92, 1268-1271.
• Dawd A. G., Gezmu T. B., Haki G. D.: Essential and toxic metals in cow’s whole milk from selected sub-cities in Addis Abeba, Ethiopia. Online Int. J. Food Sci. 2012, 1, 12-19.
• Dobrzański Z., Kołacz R., Górecka H., Chojnacka K., Bartkowiak A.: The content of microelements and trace elements in raw milk from cows in the Silesian region. Pol. J. Environ. Stud. 2005, 14, 685-689.
• Dobrzański Z., Szulc T., Kupczyński R., Kuczaj M.: Study on a content of mercury in hair, milk and blood of cows house in an urbanized area. EJPAU ser. Vet. Med. 2009, vol. 12, iss. 2 (online: http://www.ejpau.media.pl/volume12/issue2/art-02.html).
• Durgut R., Ataseven V. S., Sağkan-Öztürk A., Oztürk O. H.: Evaluation of total oxidative stress and total antioxidant status in cows with natural bovine herpesvirus-1 infection. J. Anim. Sci. 2013, 91, 3408-3412.
• Dwivedi S. K., Swarup D., Dey S., Patra R. C.: Lead poisoning in cattle and buffalo near primary lead-zinc smelter in India. Vet. Hum. Toxicol. 2001, 43, 93-94.
• Ekici H., Şimşek Ö., Arikan Ş., Eren M., Güner B.: Comparing levels of certain heavy metals and minerals and antioxidative metabolism in cows raised near and away from highways. Turk. J. Vet. Anim. Sci. 2015, 39, 322-327.
• Gong J., Xiao M.: Selenium and Antioxidant Status in Dairy Cows at Different Stages of Lactation. Biol. Trace Elem. Res. 2016, 171, 89-93.
• Kabata-Pendias A., Szteke B.: Pierwiastki śladowe w geo- i biosferze. Wyd. IUNG-PIB, Puławy 2012.
• Khan M. U., Shahbaz N., Waheed S., Mahmood A., Shinwari Z. K., Malik R. N.: Comparative health risk surveillance of heavy metals via dietary foodstuff consumption in different land-use types of Pakistan. Hum. Ecol. Risk Assess. 2016, 22, 168-186.
• Kodrik L., Wagner L., Imre K., Polyak K. F., Besenyei F., Husveth F.: The effect of highway traffic on heavy metal content of cow milk and cheese. Hun. J. Ind. Chem. 2011, 39, 15-19.
• Kołacz R., Bodak E., Dobrzanski Z., Patkowska-Sokoła B.: Trace elements in the wool of Polish merino sheep grazed in polluted and unpolluted environment. Czech J. Anim. Sci. 1999, 44, 509-514.
• Kołacz R., Dobrzański Z., Górecka H., Moryl A., Grudnik T.: Zawartość metali ciężkich w tkankach kur utrzymywanych w rejonie zagłębia miedziowego. Acta Agrophys. 2003, 1, 263-269.
• Kołacz R., Opaliński S., Dobrzański Z., Kupczyński R., Cwynar P., Durkalec M., Czaban S.: Zawartość miedzi i cynku w środowisku, w rejonie oddziaływania obiektu unieszkodliwiania odpadów wydobywczych „Żelazny Most”. Przem. Chem. 2014, 93, 1451-1454.
• Kotarska I.: Odpady wydobywcze z górnictwa miedzi w Polsce – bilans, stan zagospodarowania i aspekty środowiskowe. Cuprum 2012, 65, 45-63.
• Legislation on heavy metals in feed and food. https://ec.europa.eu/jrc/en/eurl/heavy-metals/legislation
• Leonidis A., Crivineanu V., Goran G. V., Codreanu M. D.: The level of heavy metals in blood and milk from cattle farmed near polluting industries in the province of Thessaloniki. Lucrări Stiint. Med. Vet. 2010, 43, 153-158.
• Madej J., Klimentowski S., Kołacz R., Dobrzański Z.: Rola metali ciężkich w patogenezie enzootycznej białaczki bydła (EBB). Med. Weter. 1994, 50, 374-377.
• Marczuk J., Filar J.: Ocena uszkodzenia wątroby i jej zaburzeń czynnościowych w przebiegu zespołu nadmiernej mobilizacji tłuszczu u krów mlecznych. Med. Weter. 2003, 59, 47-50.
• Medyńska A., Kabała C., Chodak T., Jezierski P.: Concentration of copper, zinc, lead and cadmium in plants cultivated in the surroundings of Żelazny Most copper ore tailings impoundment. J. Element. 2009, 14, 729-736.
• Monkiewicz J., Forster J., Geringer H., Napierała J., Jopek Z., Kucharczak E., Bas K.: Preventing overaccumulation of lead, cadmium, arsine, copper and zinc in cows kept in polluted environment. Pol. J. Environ. Study 1999, 8, 117-121.
• Noaman V.: Serum copper, zinc, and iron concentrations of Holstein dairy cows in different seasonal and physiological states. Comp. Clin. Pathol. 2014, 23, 1059-1062.
• Nwude D. O., Okoye P. A. C., Babayemi J. O.: Blood heavy metal levels in cows at slaughter at Awka Abattoir, Nigeria. Inter. J. Dairy Sci. 2010, 5, 264-270.
• Olech M., Lutnicki K., Kurek Ł., Brodzki P., Riha T., Marczuk J.: Makroelementy, wskaźniki gospodarki energetycznej i funkcji narządów miąższowych w surowicy krów suplementowanych zwiększoną dawką profilaktycznego dodatku mineralnego. Med. Weter. 2014, 70, 432-436.
• Palheta D., Taylor A.: Mercury in environmental and biological samples from a gold mining area in the Amazon region of Brazil. Sci. Total Environ. 1995, 168, 636-639.
• Pavlata L., Podhorsky A., Pechova A., Chomat P.: Differences in the occurrence of selenium, copper and zinc deficiencies in dairy cows, calves, heifers and bulls. Vet. Med.-Czech. 2005, 50, 390-400.
• Preś J., Mordak R. (red).: Wybrane elementy żywienia a problemy zdrowotne krów mlecznych. Wyd. MedPharm, Wrocław 2010.
• Ranjan R., Swarup D., Naresh R., Patra R. C.: Enhanced Erythrocytic Lipid Peroxides and Reduced Plasma Ascorbic Acid, and Alteration in Blood Trace Elements Level in Dairy Cows with Mastitis. Vet. Res. Com. 2005, 29, 27-34.
• Rogowska K. A., Monkiewicz J., Grosicki A.: Lead, cadmium, arsenic, copper and zinc contents in the hair of cattle living in the area contaminated by a copper smelter in 2006-2008. Bull. Vet. Inst., Pulawy 2009, 53, 703-706.
• Shang Y. X., Zhao Y., Qiu H. Y., Chang J. J., Chen Y. Z., Zhang H. Y.: Effects of a Food Ingredient Group on Oxidative Stress in Lead-Poisoned Mice. Integr. Med. Int. 2015, 2, 49-55.
• Shukla A., Shukla G. S., Srimal R. C.: Cadmium-induced alterations in blood-brain barrier permeability and its possible correlation with decreased microvessel antioxidant potential in rat. Hum. Exp. Toxicol. 1996, 15, 400-405.
• Sobiech P., Żarczyńska K., Rękawek W., Snarska A., Eleusizowa A., Kowalczyk E., Illek J.: Effect of parenteral supplementation of selenium and vitamin E on selected blood biochemical parameters in H-F cows during the transition period. Med. Weter. 2015, 71, 683-689.
• Spolders M., Höltershinken M., Meyer U., Rehage J., Flachowsky G.: Assessment of reference values for copper and zinc in blood serum of first and second lactating dairy cows. Vet. Med. Int., 01/2010, 2010, 194656. DOI:10.4061/2010/194656.
• Swarup D., Naresh R., Varshney V. P., Balagangatharathilagar M., Kumar P., Nandi D., Patra R. C.: Changes in plasma hormones profile and liver function in cows naturally exposed to lead and cadmium around different industrial areas. Res. Vet. Sci. 2007, 82, 16-21.
• Szkod J., Durkalec M., Kolacz R., Opalinski S., Zmudzki J.: Content of cadmium, lead and mercury in the tissues of game animals. Med. Weter. 2012, 68, 689-692.
• Tomza-Marciniak A., Pilarczyk B., Bąkowa M., Pilarczyk R., Wójcik J., Marciniak A., Hendzel D.: Relationship between selenium and selected heavy metals concentration in serum of cattle from a non-polluted area. Biol. Trace Elem. Res. 2011, 144, 517-524.
• Tracz E., Kupczyński R., Mordak R., Zawadzki M.: Analiza wybranych wskaźników metabolicznych krów utrzymywanych w różnych systemach. Acta Sci. Pol. ser. Medicina Veterinaria 2012, 11, 15-24.
• Winnicka A.: Wartości referencyjne podstawowych badań laboratoryjnych w weterynarii. Wyd. SGGW, Warszawa 2011.

Identyfikatory

DOI

10.21521/mw.5652