In vitro shoot cultures of pink rock-rose (Cistus x incanus L.) as a potential source of phenolic compounds

• Autorzy: Kubica P. , Szopa A. , Ekiert H.
• Języki publikacji: EN

Abstrakty

EN

In vitro cultures of Cistus ×incanus (pink rock-rose) were maintained on two vari­ants of Murashige and Skoog (MS) medium differing in terms of composition of plant growth regulators (PGRs): 6-benzyladenine (BA) and 1-naphthaleneacetic acid (NAA) at the following concentrations: 3 mg/L and 0, 3 mg/L and 1 mg/L, respectively, and on a variant without PGRs – a control. Cultures were maintained in a form of agar and agitated shoot cultures. The qualitative and quantitative analyzes of three groups of phenolic compounds (catechins, flavonoids, and free phenolic acids) were performed by using the HPLC-DAD technique in methanolic extracts of in vitro biomasses and of commercial plant raw material. In analyzed extracts from in vitro cultures, the presence of catechin [max. 197.80 mg / 100 g dry weight (DW)], epicatechin gallate (max. 30.74 mg / 100 g DW), gallic acid (max. 83.23 mg / 100 g DW), quercetin (max. 10.15 mg / 100 g DW), and quercitrin (max. 72.89 mg / 100 g DW) was confirmed. The quantities of accumulated compounds varied and depended on the type of in vitro culture and the concentration of PGRs in media. The highest amounts of all estimated compounds were obtained in biomasses from agar cultures cultivated on medium without PGRs in vitro. In extracts obtained from commercial raw material, gallic acid (max. 261.80 mg / 100 g DW) and quercetin (max. 255.96 mg / 100 g DW) were detected as being the dominant compounds.

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2017
• Tom: 86
• Numer: 4
• Opis fizyczny: Article 3563 [11p.],fig.,ref.

Twórcy

autor

Kubica P.

• Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College, Medyczna 9, 30-688 Cracow, Poland

autor

Szopa A.

• Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College, Medyczna 9, 30-688 Cracow, Poland

autor

Ekiert H.

• Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College, Medyczna 9, 30-688 Cracow, Poland

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Identyfikatory

DOI

10.5586/asbp.3563