Wpływ dodatku enzymów paszowych w żywieniu świń na aktywność wybranych enzymów osocza krwi

• Autorzy: Czech A. , Grela E. R. , Traczykowski A. , Stachyra K.

Warianty tytułu

EN

Effect of feed enzyme additives in pig diets on some enzymatic activity in blood plasma

• Języki publikacji: PL

Abstrakty

EN

The research objective was to determine the effect of supplemental enzymes that break down phytates or hydrolyze non-starch polysaccharide fractions in the diets of pigs under the complete production cycle (sows at gestation and lactation, growers and fatteners) on the activity of blood ALT, AST, AP and LDH. Also analyzed were the interaction between some feed additives (formic acid and its potassium salt, calcitriol) and microbial phytase or a multienzymatic preparation and their impact on the activity of these enzymes. In each of the three experiments, two control groups were formed: positive (PC) with a dicalcium phosphate (10 g kg⁻¹) supplement and negative (NC) with plant feedstuffs as a phosphorus source. Nutrient content (excluding phosphorus and calcium) at each feeding period was consistent with the Standards for Pig Feeding, 1993. In Experiment I, the NC mixture was supplemented with the following: microbial phytase (500 PU kg⁻¹) for group F, enzymes hydrolyzing non-starch polysaccharide fractions for group E, microbial phytase and enzymes hydrolyzing non-starch polysaccharide fractions for group FE, while group W received a multienzymatic preparation which comprised both microbial phytase and enzymes contributing into the non-starch polysaccharide hydrolysis (xylanase, beta-glucanase, cellulase). In Experiment II, the pig groups FM, WM, FK and WK were supplied with a mixture like in group NC with the addition of microbial phytase and a preparation including formic acid and its potassium salt for group FM, a multienzymatic preparation and a preparation with formic acid and salt for group WM, microbial phytase and calcitriol for group FK, as well as a multienzymatic preparation and calcitriol for group WK. In Experiment III, the animals from group FKM and WKM were fed the NC diet supplemented with microbial phytase, calcitriol and a preparation comprising formic acid and its potassium salt for group FKM, with a multienzymatic preparation, calcitriol and a preparation with formic acid and its potassium salt for group WKM. Blood was collected from 8 gilts from each group at 84 days of gestation and 21 days of lactation, from 8 growing pigs from each group at the starter period (56 raising day), the grower period (91 days of age) and finisher period (154 days of age). Blood was examined to establish the activity of ALT, AST, AP and LDH using the colorimetric assay with Cormay monotests. The results of the present research conducted on the pigs at the complete production cycle, fed diets deprived of a calcium phosphate content but supplemented with microbial phytase, enzymes hydrolyzing non-starch polysaccharide fractions, calcitriol or a preparation comprising formic acid and its potassium salt have given evidence of a stimulating effect of the employed additives on the activity of enzymes from the transferase enzyme group, AP and LDH. The animals from group W showed a significant increase in AST, AP and LDH activity, primarily in the fatteners. The activity of AST and LDH in the blood of pigs from the groups FKM, WKM proved to be significantly higher (p ≤ 0.05) during the whole cycle as compared to the animals from the NC group. In none of the groups under study were deviations noted from the reference values for the activity of enzymes analyzed in the present research.

Słowa kluczowe

PL

trzoda chlewna; zywienie zwierzat; dodatki paszowe; enzymy paszowe; fitaza mikrobiologiczna; kwas mrowkowy; sol potasowa kwasu mrowkowego; preparaty wieloenzymatyczne; kalcytriol; badania krwi; aminotransferaza alaninowa; aminotransferaza asparaginianowa; fosfataza zasadowa; dehydrogenaza mleczanowa; aktywnosc enzymatyczna

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2011
• Tom: 67
• Numer: 08
• Opis fizyczny: s.560-565,tab.,bibliogr.

Twórcy

autor

Czech A.

• Katedra Biochemii i Toksykologii, Wydział Biologii i Hodowli Zwierząt, Uniwersytet Przyrodniczy w Lublinie ,ul.Akademicka 13, 20-950 Lublin

autor

Grela E. R.

autor

Traczykowski A.

autor

Stachyra K.

Bibliografia

• 1. Biehl R. R., Baker D. H.: Efficacy of supplemental l-alpha-hydroxycholecalciferol and microbial phytase for young pigs fed phosphorus- or amino acid-deficient corn-soybean meal diets. J. Anim. Sci. 1996, 74, 2960-2966.
• 2. Biehl R. R., Baker D. H., Deluca H. F.: Activity of various hydroxylated vitamin D3 analogs for improving phosphorus utilization in chicks receiving diets adequate in vitamin D₃. Br. Poultry Sci. 1998, 39, 408-412.
• 3. Czech A.: Efektywność fitazy w żywieniu zwierząt. Medycyna Wet. 2007, 63, 1034-1039.
• 4. Czech A.: Effect of microbial phytase and formic acid supplementation in sow diets on biochemical parameters of blood. Ann. Anim Sci. 2004, 2, 105-109.
• 5. Dembińska-Kieć A., Naskalski W. J.: Diagnostyka laboratoryjna z elementami biochemii klinicznej. Urban&Partner, Wrocław 2002.
• 6. Eeckhout W., De Paepe M.: In vitro and in vivo comparison of microbial and plant phytase, [w:] Coelho M. B., Kornegay E. T. (eds): Phytase in Animal Nutrition and Waste Management. BASF Corporation, Mount Olive, NJ 1996, 237-240.
• 7. Fang Z. F., Peng J., Liu Z. L., Liu Y. G.: Responses of non-starch polysaccharide-degrading enzymes on digestibility and performance of growing pigs fed a diet based on corn, soya bean meal and Chinese double-low rapeseed meal. J. Anim. Physiol. Anim. Nutr. 2007, 91, 361-368.
• 8. Friendship R. M., Henry S. C.: Cardiovascular system, haematology and clinical chemistry, [w:] Leman A. D., Straw B. E., Mengeling W. L., D'Allaire S., Taylor D. J. (eds.): Diseases of Swine. Iowa State Univ. Press 1996, 3-11.
• 9. Grela E. R., Matras J., Czech A., Krasucki W.: Influence of microbial phytase supplementation to diet with high or low native phytase activity on sow reproductive traits and colostrum and milk composition. J. Anim. Feed Sci. 2010, 19, 418-429.
• 10. Jongbloed A. W., Mroz Z., van der Weij-Jongbloed R., Kemme P. A.: The effects of microbial phytase, organic acids and their interaction in diets for growing pigs. Livest. Prod. Sci. 2000, 67, 113-122.
• 11. Lindberg J. E., Lyberg K., Sands J.: Influence of phytase and xylanase supplementation of a wheat-based diet on ileal and total tract digestibility in growing pigs. Livest. Sci. 2007, 109, 268-270.
• 12. Metzler-Zebeli B. U., Vahjen W., Baumgärtel T., Rodehutscord M., Mosenthin R.: Ileal microbiota of growing pigs fed different dietary calcium phosphate levels and phytase content and subjected to ileal pectin infusion. Anim. Sci. 2010, 88, 147-158.
• 13. Normy Żywienia świń: Instytut Fizjologii i Żywienia Zwierząt im. Jana Kielanowskiego, PAN, Omnitech Press, Warszawa 1993.
• 14. Rokicki E., Kolbuszewski T.: Higiena zwierząt. Fundacja "Rozwój SGGW", Warszawa 1996.
• 15. Sands J. S., Ragland D., Baxter C., Joern B. C., Sauber T. E., Adeola O.: Phosphorus bioavailability, growth performance, and nutrient balance in pigs fed high available phosphorus corn and phytase. J. Anim. Sci. 2001, 79, 2134-2142.
• 16. Stahl C. H., Roneker K. R., Pond W. G., Lei X. G.: Effects of combining three fungal phytases with a bacterial phytase on plasma phosphorus status of weanling pigs fed a corn-soy diet. J. Anim. Sci. 2004, 82, 1725-1731.
• 17. Waldroup P. W., Kersey J. H., Saleh E. A., Fritts C. A., Yan F., Stilborn H. L., Crum R. C., Raboy Jr. V.: Nonphytate phosphorus requirement and phosphorus excretion of broilers fed diets composed of normal or high available phosphate corn with and without microbial phytase. Poult. Sci. 2000, 79, 1451-1459.
• 18. Winnicka A.: Wartości referencyjne podstawowych badań laboratoryjnych w weterynarii. Wydawnictwo SGGW, Warszawa 2008.