PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2018 | 17 | 1 |

Tytuł artykułu

Simple verification of in vitro - grown clones of the genus Drosera L. using its molecular markers

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Drosera L. is a genus of carnivorous plants that comprises approximately 250 species, although this number is probably not complete. Some of these taxa exhibit only small differences in morphological traits that can be partly influenced if the taxa are propagated in vitro. Here, we focus on the verification of putative clones of Drosera spathulata Labill., Drosera rotundifolia L. and Drosera binata var. Dichotoma species cultivated in vitro using molecular markers covering the internal transcribed spacer (ITS) region of 45S ribosomal DNA (rDNA). Following the polymerase chain reaction (PCR) amplification of ~360-bp DNA fragments and sequencing, the sequences were aligned with corresponding sequences in the National Center for Biotechnology Information (NCBI) database. In addition, each of tested PCR amplicons had a specific restriction profile that predominantly enables the differentiation of D. rotundifolia and D. spathulata; the shape of the leaves does not have to be a clear morphologically distinguishable trait.

Wydawca

-

Rocznik

Tom

17

Numer

1

Opis fizyczny

p.159-164,fig.,ref.

Twórcy

  • Institute of Plant Genetics and Biotechnology, PSBC, Slovak Academy of Sciences, Akademicka 2, P.O.Box 39A, 950 07 Nitra, Slovak Republic
autor
  • NAFC Research Institute for Animal Production, Nitra, Hlohovska 2, 951 41 Luzianky, Slovak Republic
  • Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University, Nabrezie mladeze 91, 949 74 Nitra, Slovak Republic
autor
  • Institute of Plant Genetics and Biotechnology, PSBC, Slovak Academy of Sciences, Akademicka 2, P.O.Box 39A, 950 07 Nitra, Slovak Republic
autor
  • Institute of Plant Genetics and Biotechnology, PSBC, Slovak Academy of Sciences, Akademicka 2, P.O.Box 39A, 950 07 Nitra, Slovak Republic

Bibliografia

  • Baldwin, B.G., Sanderson, M.J., Porter, J.M., Wojciechowski, M.F., Campbell, C.S., Donoghue, M.J. (1995). The ITS region of nuclear ribosomal DNA – a valuable source of evidence on angiosperm phylogeny. Ann. Mo. Bot. Gard., 82, 247–277.
  • Batagin, K.D., de Almeida, C.V., Tanaka, F.A.O., de Almeida, M. (2009). Morphological alterations in leave of micropropagated pineapple plants cv. IAC “Gomode-mel” acclimatizated in different conditions of luminosity. Acta Bot. Bras., 23, 85–92.
  • Bekesiova, I., Nap, J.P., Mlynarova, L. (1999). Isolation of high quality DNA and RNA from leaves of the carnivorous plant Drosera rotundifolia. Plant Mol. Biol. Rep., 17, 269–277.
  • Bobak, M., Blehova, A., Kristin, J., Ovecka, M., Samaj, J. (1995). Direct plant regeneration from leaf explants of Drosera rotundifolia cultured in vitro. Plant Cell Tissue Organ Cult., 43, 43–49.
  • Conran, J.G., Jaudzems, V.G., Hallam, N.D. (1997). Droseraceae germination patterns and their taxonomic significance. Bot. J. Linn. Soc., 123, 211–223.
  • Fineschi, S., Anzidei, M., Cafasso, D., Cozzolino, S., Garfi, G., Pastorelli, R., Salvini, D., Taurchini, D., Vendramin, G.G. (2002). Molecular markers reveal a strong genetic differentiation between two European relic tree species: Zelkova abelicea (Lam.) Boissier and Z. sicula Di Pasquale, Garfi & Quezel (Ulmaceae). Conserv. Genet., 3, 145–153.
  • Gonella, P.M., Fleischmann, A., Rivadavia, F., Neill, D.A., Sano, P.T. (2016). A revision of Drosera (Droseraceae) from the central and northern Andes, including a new species from the Cordillera del Cóndor (Peru and Ecuador). Plant Syst. Evol., 302, 1419–1432.
  • Gonella, P.M., Rivadavia, F., Fleischmann, A. (2015). Drosera magnifica (Droseraceae): the largest New World sundew, discovered on Facebook. Phytotaxa, 220, 257–267.
  • Matyasek, R., Renny-Byfield, S., Fulnecek, J., Macas, J., Grandbastien, M.A., Nichols, R., Leitch, A., Kovarik, A. (2012). Next generation sequencing analysis reveals a relationship between rDNA unit diversity and locus number in Nicotiana diploids. BMC Genomics, 13, 722.
  • Kondo, K. (1976). A cytotaxonomic study in some species of Drosera. Rhodora, 78, 532–541.
  • Renner, T., Specht, C.D. (2011). A sticky situation: assessing adaptations for plant carnivory in the Caryophyllales by means of stochastic character mapping. Int. J. Plant Sci., 172, 889–901.
  • Rivadavia, F., Gonella, P.M., Sano, P.T., Fleischmann, A. (2014). Elucidating the controversial Drosera montana complex (Droseraceae): a taxonomic revision. Phytotaxa, 172, 141–175.
  • Rivadavia, F., Kondo, K., Kato, M., Hasebe, M. (2003). Phylogeny of the sundews, Drosera (Droseraceae), based on chloroplast rbcL and nuclear 18S ribosomal DNA sequences. Am. J. Bot., 90, 123–130.
  • Rosato, M., Kovarik, A., Garilleti, R., Rossello, J.A. (2016). Conserved organisation of 45S rDNA sites and rDNA gene copy number among major clades of early land plants. Plos One, 11, e0162544.
  • Thompson, J.D., Higgins, D.G., Gibson, T.J. (1994). CLUSTAL-W – Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acid. Res., 22, 4673–4680.
  • Takahashi, H., Sohma, K., 1982. Pollen morphology of the Droseraceae and its related taxa. Science reports of the
  • research Institutes Toboku University, 4th Series, Biology, 38, 81–156
  • Trejgell, A., Dąbrowska, A., Tretyn, A. (2012). Micropropagation and influence of in vitro culture on development of Cirsium pannonicum (L. f.) link regenerants. Acta Sci. Pol. Hortorum Cultus, 11, 81–90.
  • Tungkajiwangkoon S., Shirakawa, J., Azumatani, M., Hoshi, Y. (2016). Breeding and cytogenetic characterizations of new hexaploid Drosera strains colchicines induced from triploid hybrid of D. rotundifolia and D. spatulata. Cytologia, 81, 263–269.
  • Williams, S.E., Albert, V.A., Chase, M.W. (1994). Relationships of Droseraceae – a cladistic-analysis of rbcL sequence and morphological data. Am. J. Bot., 81, 1027–1037.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-990685e8-5933-4f8a-bedb-c7e8fb250a2c
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.