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2016 | 85 | 3 |
Tytuł artykułu

EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.

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Treść / Zawartość
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Języki publikacji
EN
Abstrakty
EN
In this study, when germinated Triticum aestivum L. seeds were treated with 0, 2, 4 and 6 mM ethyl glycol tetraacetic acid (EGTA), root growth was suppressed and the mitotic index decreased. These inhibitory effects were positively correlated with EGTA concentration. RT-PCR analysis revealed that the expression of several gene markers related to the G1/S transition of the cell cycle were significantly downregulated. Confocal microscopy of Fluo-3/AM-stained roots showed chelation of nearly all of the Ca2+ within the root meristematic regions. Both random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques showed significant increases in the levels of genomic DNA polymorphisms and degree of DNA methylation. The study provides information concerning the impact of Ca²+) chelator, EGTA, on the growth, expression of cell cycle transition marker genes, and changes in DNA structure and methylation in the wheat roots.
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EN
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Tom
85
Numer
3
Opis fizyczny
Article 3502 [14p.], fig.,ref.
Twórcy
autor
  • College of Life Sciences, Capital Normal University, No. 105, Xi San Huan Bei Lu, Beijing 100048, China
autor
  • College of Life Sciences, Capital Normal University, No. 105, Xi San Huan Bei Lu, Beijing 100048, China
autor
  • College of Life Sciences, Capital Normal University, No. 105, Xi San Huan Bei Lu, Beijing 100048, China
autor
  • Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
autor
  • College of Life Sciences, Capital Normal University, No. 105, Xi San Huan Bei Lu, Beijing 100048, China
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Typ dokumentu
Bibliografia
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