The activity of lysosomal enzymes in blood serum, liver, kidney and skeletal muscle of rabbits divergently selected for locomotor activity in open-field test

• Autorzy: Jozwik A. , Sliwa-Jozwik A. , Jezierski T. , Daniewski W. , Gorecka A. , Kolataj A.

Warianty tytułu

PL

Aktywność enzymów lizosomowych w wybranych tkankach i surowicy krwi królików selekcjonowanych rozbieżnie na aktywność ruchową w otwartym polu (test OF)

• Języki publikacji: EN

Abstrakty

EN

Twenty males were used from generation 8 of rabbits divergently selected for high (line H, n=10) vs. low (line L, n=10) locomotor activity in the open-field (OF-test). At the age of 98 days, blond samples were withdrawn from the ear vein and the rabbits were killed. Immediately, liver, kidney and the left femoral muscle samples were excised. In lysosomal fractions of blood serum and three tissues mentioned the activity of lysosomal enzymes AlaAP, LeuAP, ArgAP, Cat D and L,AcP, EL, LAL, BGRD, BGAL, BGLU, aGlu, MAN and HEX was determined. Significant interlinie differences were identified in activity of blood and tissue enzymes in question. The results suggest that the divergent selection for high vs. low locomotor activity in the open-field (OF-test) alters the lysosomal degradation processes in the organism.

PL

Badania przeprowadzono na 20 samcach należących do ósmego pokolenia królików rozbieżnie selekcjonowanych na wysoką (H, n= 10) lub niską (L, n= 10) aktywność ruchową w otwartym polu (test OF). Krew z żyły usznej pobrano w 98 dniu życia królików, zwierzęta zdekapitowano i natychmiast pozyskano skrawki wątroby, nerki i mięśnia lewego uda, które poddano homogenizacji. We frakcjach lizosomowych czterech wymienionych materiałów oznaczono aktywność następujących enzymów: AlaAP, LeuAP, ArgAP, Cat D i L, AcP, EL, LAL, BGRD, BGAL, BGLU, aGlu, MAN i HEX. Między dwiema selekcjonowanymi liniami królików stwierdzono istotne różnice w aktywności badanych enzymów. Uzyskane wyniki sugerują, że rozbieżna selekcja na wysoką i niską aktywność ruchową z zastosowaniem testu otwartego pola wpływa na przebieg lizosomowych procesów degradacyjnych w organizmie.

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2009
• Tom: 27
• Numer: 3
• Opis fizyczny: p.249-257,fig.,ref.

Twórcy

autor

Jozwik A.

• Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Sliwa-Jozwik A.

• Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Jezierski T.

• Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Daniewski W.

• Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Gorecka A.

• Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Kolataj A.

• Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

Bibliografia

• Barrett A.J., Heath M.F., 1972 – Lysosomal enzymes. In: Lysosomes. A Laboratory Handbook. J.T. Dingle, ed., North-Holland Publ. Co., Amsterdam, 19-135.
• Beaufay H., 1972 – Methods for the isolation of lysosomes. In: Lysosomes. A Laboratory Handbook. J.T. Dingle, ed., North-Holland Publ. Co., Amsterdam, London, 46-135.
• Brun J.M., Theau -Clement M., Bolet G., 2002 – Evidence for heterosis and Materna effects on rabbit semen characteristics. Animal Research 51, 433-442.
• Cabrita M.A., Hobman T.C., Hogue D.L., King K.M., Cass C.E., 1999 – Mouse transporter protein, a membrane protein that regulates cellular multidrug resistance, is localized to lysosomes. Cancer Research 59, 4890-4897.
• Choleris E., Thomas A.W., Kavaliers M., Prato F.S., 2001 – A detailed ethological analysis of the mouse open field test – effects of diazepam, chlordiazepoxide and an extremely low frequency pulsed magnetic field. Neuroscience and Biobehavioral Reviews 25, 235-260.
• Daniewski W., Jezierski T., 2003 – Effectiveness of divergent selection for open-field activity in rabbits and correlated response for body weight and fertility. Behavior Genetics 33, 337-345.
• Denenberg V.H., 1977 – Assessing the effects of early experience. In: Methods in Psychobiology. R.D. Myers, ed. Academic Press, 127-147.
• Dittmer F., Ulbrich E. J., Hafner A., Schmahl W., Meister T., Pohlmann R.,von Figura K., 1999 – Alternative mechanisms for trafficking of lysosomal enzymes in mannowe 6-phosphate receptor-deficient mice are cell type-specific. Journal of Cell Science 112, 1591-1597.
• Ferrante V., Verga M., Canali E., Mattiello S., 1992 – Rabbits kept in cages and In floor pens - reactions in the open-field test. Journal of Applied Rabbit Research 15, 700-707.
• Hall C.S., 1934 – Emotional behavior in the rat. I. Defecation and urination as measures of individual differences in emotionality. Journal of Comparative Psychology 18, 385-403.
• Itabe H., Suzuki K., Tsukamoto Y., Komatsu R., Ueda M., Mori M., Higashi Y.,Takano T., 2000 – Lysosomal accumulation of oxidized phosphatidylocholine-apolipoprotein B complex in macrophages – intracellular fate of oxidized low-density lipoprotein. Biochimica et Biophysica Acta 1487, 233-245.
• JÓŹWIK A., BAGNICKA E., ŚLIWA-JÓŹWIK A., STRZAŁKOWSKA N., SŁONIEWSKI K.,KRZYŻEWSKI J., KOŁĄTAJ A., 2004 – Activity of selected glycosidases of whole milk in cows as related to feeding season (autumn/winter vs. spring/summer). Animal Science Papers and Reports 22, 673-677.
• Jóźwik A., Śliwa -Jóźwik A., Bagnicka E., Kołątaj A., 2003 – The influence of selection on reaction to stress in mice. IX. Effect of dietary protein level on activity of lysosomal enzymes In liver and kidney. Journal of Animal Breeding and Genetics 120, 124-131.
• Kirschke H., Wiederanders B., 1984 – Methoden zur Aktivitätsbestimmung von Proteinases. Martin Luther Universität, Halle-Wittenberg, Wissenschaftliche Beitrage. Halle/Salle, pp. 11-17.
• Kołątaj A., 1993 – Pochwała stresu. Wydawnictwo Naukowe, Kielce, 1-205.
• KOŁĄTAJ A., ŚLIWA-JÓŹWIK A., JÓŹWIK A., 2001 – The lysosomal cell complex as a stress response indicator. Animal Science Papers and Reports 19, 177-192.
• Konecka A.M., Jezierski T., Śliwa -Jóźwik A., Jóźwik A., Kołątaj A., 2002 – The effect of cholesterol-enriched diet on the activity of some lysosomal enzymes in the liver and blond plasma of rabbits. Archiv fuer Tierzucht 45, 199-203.
• Lange Y., Ye J., Steck T.L., 1998 – Circulation of cholesterol between lysosomes and the plasma membrane. Journal of Biological Chemistry 273, 18915-18922.
• Langner J., Wakil A., Zimmermann M., Ansorge S., Bohle y P., Kirschke H.,Wiederanders B., 1973 – Aktivitätsbestimmung proteolytischer Enzyme mit Azokasein als Substrat. Acta Biologica et Medica Germanica 31, 1-18.
• Lankoff A., Kołątaj A., 2000 – Influence of microcystine-YR and nodularin on the activity of some glucosidases in mouse liver. Toxicology 146, 177-185.
• Luzio J.P., Rous B.A., Bright N.A., Pryor P.R., Mullock B.M., 2000 – Lysosomeendosome fusion and lysosome biogenesis. Journal of Cell Science 113, 1515-1524.
• McDonald J.K., Barrett A.J., 1986 – Mammalian Proteases – a Glossary and Bibliography. Volume 2 - Exopeptidases, Academic Press, London.
• Miszczuk -Jamska B., Chevalier F., Maire I., Bembinska -Kiec A., 1993 – Acid and neutral lipase activity in lymphocytes of patients with increased serum cholesterol and triglyceride level. Journal of Physiology and Pharmacology 44, 345-350.
• Molano F., Saborido A., Delgado J., Morano M., Megias A., 1999 – Rat liver lysosomal and mitochondrial activities are modified by anabolic-androgenic steroids. Medicine and Science in Sports and Exercise 31, 243-251.
• Nakano A., Marks D.L., Tietz P.S., de Grode P.C., LaRusso N.F., 1995 – Quantitative importance of biliary excretion to the turnover of hepatic lysosomal enzymes. Hepatology 22, 262-266.
• Ossenkopp K.P., Rabi Y.J., Eckel L.A., Hargreaves E.L., 1994 – Reductions in body temperature and spontaneous activity in rats exposed to horizontal rotation - abolition following chemical labyrinthectomy. Physiology and Behavior 56, 319-324.
• Prut L., Belzung C., 2003 – The open field as a paradigm to measure the effects of drugs on anxiety-like behaviours – a review. European Journal of Pharmacology 463, 3-33.
• Ramos A., Correia E.C., Izidio G.S.; Brüske G.R., 2003 – Genetic selection of two new rat lines displaying different levels of anxiety-related behaviors. Behavior Genetics 33, 657-668.
• Rider E.D., Ikegami M., Pienkerton K.E., Peake J.L., Jobe A.H., 2000 – Lysosomes from rabbit type II cells catabolize surfactant lipids. American Journal of Physiology Lung cellular and molecular physiology 278, L68-L74.
• Schmitt U., Hiemke C., 1998 – Strain differences in open-field and elevated plus-maze behavior of rats without and with pretest handling. Pharmacology, Biochemistry and Behavior 59, 807-811.
• Stam C., de Bruin J., van Haelst A., van der Gugten J., Kalsbeek A., 1989 – Influence of the mesocortical dopaminergic system on activity, food hoarding, social-agonistic behavior, and spatial delayed alternation in male rats. Behavioral Neuroscience 103, 24-35.
• Śliwa -Jóźwik A., Jóźwik A., Kołątaj A., 2002 – Influence of exogenous glutathione GSH as stressfactor, on the activity of lysosomal enzymes in some organs of mice. Archiv fuer Tierzucht 45, 307-314.
• Witek B., 2000 – Fizjologia reakcji stresowej i model lizosomalnego układu degradacyjnego (Physiology of stress reaction and model of lysosomal degradative system). In Polish. Published by the University of Agriculture (SGGW) Press. Warszawa, pp. 5-60.
• Zelnik J., Kołątaj A., Rafay J., Parkayi V., Bulla J., Flak P., Tokarski J., 1990 – Motor activity of rabbits of various genetic groups in the open field. Journal of Animal Breeding and Genetics 107, 465-469.