PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2011 | 33 | 6 |

Tytuł artykułu

Differential expression of proteins in maize roots in response to abscisic acid and drought

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Roots are highly sensitive organ in plant response to drought, which commonly inhibits root growth. However, less is known about the effect of ABA on root protein expression induced by drought. To help clarify the role of ABA in protein expression of root response to drought, root protein patterns were monitored using a proteomic approach in maize ABA-deficient mutant vp5 and its wild-type Vp5 exposed to drought. Two-dimensional electrophoresis was used to identify droughtresponsive protein spots in maize roots. After coomassie brilliant blue staining, approximately 450 protein spots were reproducibly detected on each gel, wherein 22 protein spots related to ABA or drought were identified using MALDI-TOF MS. Results showed that the 22 proteins are involved in such several cellular processes as energy and metabolism, redox homeostasis and regulatory. An anionic peroxidase and two putative uncharacterized proteins were up-regulated by drought in ABA-dependent way; A glycine-rich RNA binding protein 2, pathogenesis-related protein 10, an enolase, a serine/threonine-protein kinase receptor and a cytosolic ascorbate peroxidase were up-regulated by drought in both ABA-dependent and ABAindependent way; a nuclear transport factor 2, a nucleoside diphosphate kinase, a putative uncharacterized protein and a peroxiredoxin-5 were up-regulated by drought in ABAindependent way; a superoxide dismutase 4A, a VAP27-2, a transcription factor BTF3, a glutathione S-transferase GSTF2 and a putative uncharacterized protein were up-regulated by drought in ABA-dependent way, but not exogenous ABA treatment in the absence of drought; a O-methyltransferase and a putative uncharacterized proteins were down-regulated by ABA and drought. The identification of some novel proteins in the drought response provides new insights that can lead to a better understanding of the molecular basis of root drought tolerance.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

33

Numer

6

Opis fizyczny

p.2437-2446,fig.,ref.

Twórcy

autor
  • College of Life Science, Henan Agricultural University, Zhengzhou 450002, China
  • Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou, China
autor
  • College of Life Science, Henan Agricultural University, Zhengzhou 450002, China
autor
  • College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China
  • Huanghuaihai Regional Innovation Center for Maize Technology, Ministry of Agriculture, Zhengzhou, China
autor
  • College of Life Science, Henan Agricultural University, Zhengzhou 450002, China
autor
  • College of Life Science, Henan Agricultural University, Zhengzhou 450002, China
autor
  • College of Life Science, Henan Agricultural University, Zhengzhou 450002, China
autor
  • College of Life Science, Henan Agricultural University, Zhengzhou 450002, China
autor
  • College of Life Science, Henan Agricultural University, Zhengzhou 450002, China
autor
  • College of Life Science, Henan Agricultural University, Zhengzhou 450002, China

Bibliografia

  • Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399
  • Buchanan CD, Lim S, Salzman RA, Kagiampakis I, Morishige DT, Weers BD, Klein RR, Pratt LH, Cordonnier-Pratt MM, Klein PE, Mullet JE (2005) Sorghum bicolor’s transcriptome response to dehydration, high salinity and ABA. Plant Mol Biol 5:699–720
  • Cho SM, Shin SH, Kim KS, Kim YC, Eun MY, Cho BH (2004) Enhanced expression of a gene encoding a nucleoside diphosphate kinase 1 (OsNDPK1) in rice plants upon infection with bacterial pathogens. Mol Cell 18:390–395
  • Comstock JP (2002) Hydraulic and chemical signalling in the control of stomatal conductance and transpiration. J Exp Bot 53:195–200
  • Custers JH, Melchers LS, Tigelaar H, Bade JB, Spiegeler JJ, van Der Meijs PJ, Simons BH, Stuiver MH (2002) T-DNA tagging of a pathogen inducible promoter in Arabidopsis thaliana. Mol Plant Pathol 3:239–249
  • Davletova S, Rizhsky L, Liang H, Zhong S, Oliver DJ, Coutu J, Shulaev V, Schlauch K, Mittler R (2005) Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis. Plant Cell 17:268–281
  • Dietz KJ, Jacob S, Oelze ML, Laxa M, Tognetti V, de Miranda SMN, Baier M, Finkemeier I (2006) The function of peroxiredoxins in plant organelle redox metabolism. J Exp Bot 57:1697–1709
  • Dooki AD, Mayer-Posner FJ, Askari H, Zaiee A, Salekdeh GH (2006) Proteomic responses of rice young panicles to salinity. Proteomics 6:6498–6507
  • Dubos C, Plomion C (2001) Drought differentially affects expression of a PR-10 protein, in needles of the maritime pine (Pinus pinaster Ait.) seedlings. J Exp Bot 52:1143–1154
  • Forsthoefel NR, Cushman MAF, Cushman JC (1995) Posttranscriptional and posttranslational control of enolase expression in the facultative grassulacean acid metabolism plant Mesembryanthemum crystallinum L. Plant Physiol 108:1185–1195
  • Gorantla M, Babu PR, Lachagari VBR, Feltus FA, Paterson AH, Reddy AR (2005) Functional genomics of drought-stress response in rice: transcript mapping of annotated unigenes of an indica rice (Oryza sativa L. cv. Nagina 22). Curr Sci 289:496–514
  • Guan LQ, Scandalios JG (1998) Two structurally similar maize cytosolic superoxide dismutase genes, Sod4 and Sod4A, respond differentially to abscisic acid and high osmoticum. Plant Physiol 117:217–224
  • Guillet-Claude C, Birolleau-Touchard C, Manicacci D, Fourmann M, Barraud S, Carret V, Martinant JP, Barrie‘ re Y (2004) Genetic diversity associated with variation in silage corn digestibility for three O-methyltransferase genes involved in lignin biosynthesis. Theor Appl Genet 110:126–135
  • Hajheidari M, Salekdeh GH, Heidari M, Abdollahian-Noghabi M, Sadeghian SY (2005) Proteome analysis of sugar beet leaves under drought stress. Proteomics 5:950–960
  • Halusková L, Valentovicová K, Huttová J, Mistrík I, Tamás L (2009) Effect of abiotic stresses on glutathione peroxidase and glutathione S-transferase activity in barley root tips. Plant Physiol Biochem 47:1069–1074
  • Hu X, Li Y, Li C, Yang H, Wang W, Lu M (2010a) Characterization of small heat shock proteins associated with maize tolerance to combined drought and heat stress. J Plant Growth Regul 29:455–464
  • Hu X, Liu R, Li Y, Wang W, Tai F, Xue R, Li C (2010b) Heat shock protein 70 regulates the abscisic acid-induced antioxidant response of maize to drought and heat stress combination. Plant Growth Regul 60:225–235
  • Jeong JS, Kim YS, Baek KH, Jung H, Ha SH, Do Choi Y, Kim M, Reuzeau C, Kim JK (2010) Root-specific expression of Os-NAC10 improves drought tolerance and grain yield in rice under field drought conditions. Plant Physiol 153:185–197
  • Jiang HW, Liu MJ, Chen IC, Huang CH, Chao LY, Hsieh HL (2010) A glutathione S-transferase regulated by light and hormones participates in the modulation of Arabidopsis seedling development. Plant Physiol 154:1646–1658
  • Kellos T, Tímár I, Szilágyi V, Szalai G, Galiba G, Kocsy G (2008) Stress hormones and abiotic stresses have different effects on antioxidants in maize lines with different sensitivity. Plant Biol (Stuttg) 10:563–572
  • Kim JY, Park SJ, Jang B, Jung CHH, Ahn SJ, Goh CHH, Cho K, Han O, Kang H (2007) Functional characterization of a glycine-rich RNA-binding protein2 in Arabidopsis thaliana under abiotic stress conditions. Plant J 50:439–451
  • Koussevitzky S, Suzuki N, Huntington S, Armijo L, Sha W, Cortes D, Shulaev V, Mittler R (2008) Ascorbate peroxidase 1 plays a key role in the response of Arabidopsis thaliana to stress combination. J Biol Chem 283:34197–34203
  • Kwak KJ, Kim YO, Kang H (2005) Characterization of transgenic Arabidopsis plants overexpressing GR-RBP4 under high salinity, dehydration, or cold stress. J Exp Bot 56:3007–3016
  • Lee J, Parthier B, LiJbler M (1996) Jasmonate signalling can be uncoupled from abscisic acid signaling in barley: identification of jasmonate-regulated transcripts which are not induced by abscisic acid. Planta 199:625–632
  • Lee DG, Ahsan N, Lee SH, Kang KY, Bahk JD, Lee IJ, Lee BH (2007) A proteomic approach in analyzing heat-responsive proteins in rice leaves. Proteomics 7:3369–3383
  • Liu X, Huang B, Lin J, Fei J, Chen Z, Pang Y, Sun XF, Tang KX (2006) A novel pathogenesis-related protein (SsPR10) from Solanum surattense with ribonucleolytic and antimicrobial activity is stress- and pathogen-inducible. J Plant Physiol 163:546–556
  • Mangeon A, Junqueira RM, Sachetto-Martins G (2010) Functional diversity of the plant glycine-rich proteins superfamily. Plant Signal Behav 5:99–104
  • Moons A, Prinsen E, Bauw G, Van Montagu M (1997) Antagonistic effects of abscisic acid and jasmonates on salt stress-inducible transcripts in rice roots. Plant Cell 9:2243–2259
  • Mowla SB, Thomson JA, Farrant JM, Mundree SG (2002) A novel stress-inducible antioxidant enzyme identified from the resurrection plant Xerophyta viscosa Baker. Planta 215:716–726
  • Roberts E, Kolattukudy PE (1989) Molecular cloning, nucleotide sequence, and abscisic acid induction of a suberization-associated highly anionic peroxidase. Mol Gen Genet 217(2–3): 223–232. doi:10.1007/BF02464885
  • Robichaud CS, Wang J, Sussex IM (1980) Control of in vitro growth of viviparous embryo mutants of maize by abscisic acid. Dev Genet 1:325–330
  • Salekdeh GH, Siopongco J, Wade LJ, Ghareyazie B, Bennett J (2002) Proteomic analysis of rice leaves during drought stress and recovery. Proteomics 2:1131–1145
  • Shao HB, Chu LY, Jaleel CA, Zhao CX (2008) Water-deficit stressinduced anatomical changes in higher plants. Comptes Rendus Biologies 331:215–225
  • Sharp RE, LeNoble ME (2002) ABA, ethylene and the control of shoot and root growth under water stress. J Exp Bot 53:33–37
  • Sharp RE, Poroyko V, Hejlek LG, Spollen WG, Springer GK, Bohnert HJ, Nguyen HT (2004) Root growth maintenance during water deficits: physiology to functional genomics. J Exp Bot 55:2343–2351
  • Shinozaki K, Yamaguchi-Shinozaki K (2007) Gene networks involved in drought stress response and tolerance. J Exp Bot 58:221–227
  • Spollen WG, LeNoble ME, Samuels TD, Bernstein N, Sharp RE (2000) Abscisic acid accumulation maintains maize primary root elongation at low water potentials by restricting ethylene production. Plant Physiol 122:967–976
  • Taniguchi YY, Taniguchi M, Tsuge T, Oka A, Aoyama T (2010) Involvement of Arabidopsis thaliana phospholipase Df2 in root hydrotropism through the suppression of root gravitropism. Planta 231:491–497
  • Teichmann T, Guan CH, Kristoffersen P, Muster G, Tietz O, Palme K (1997) Cloning and biochemical characterization of an anionic peroxidase from Zea mays. Eur J Biochem 247:826–832
  • Torres GAM, Pflieger S, Corre-Menguy F, Mazubert C, Hartmann C, Christine Lelandais-Brière C (2006) Identification of novel drought-related mRNAs in common bean roots by differential display RT-PCR. Plant Sci 171:300–307
  • Vincent D, Lapierre C, Pollet B, Cornic G, Negroni L, Michel Zivy M (2005) Water deficits affect caffeate O-methyltransferase, lignification, and related enzymes in maize leaves. A proteomic investigation. Plant Physiol 137:949–960
  • Wang W, Vignani R, Scali M, Cresti M (2006) A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis. Electrophoresis 27:2782–2786. doi:10.1002/elps.200500722
  • Wang W, Bianchi L, Scali M, Liu LW, Bini L, Cresti M (2009) Proteomic analysis of b-1, 3-glucanase in grape berry tissues. Acta Physiol Plant 31:597–604
  • Xie YR, Chen ZY, Brown RL, Bhatnagar D (2010) Expression and functional characterization of two pathogenesis-related protein 10 genes from Zea mays. J Plant Physiol 167:121–130
  • Xiong L, Wang RG, Mao GH, Koczan JM (2006) Identification of drought tolerance determinants by genetic analysis of root response to drought stress and abscisic acid. Plant Physiol 142:1065–1074
  • Yang L, Zheng B, Mao C, Qi X, Liu F, Wu P (2004) Analysis of transcripts that are differentially expressed in three sectors of the rice root system under water deficit. Mol Gen Genomics 272:433–442
  • Yazaki J, Shimatani Z, Hashimoto A, Nagata Y, Fujii F, Kojima K, Suzuki K, Taya T, Tonouchi M, Nelson C, Nakagawa A, Otomo Y, Murakami K, Matsubara K, Kawai J, Carninci P, Hayashizaki Y, Kikuchi S (2004) Transcriptional profiling of genes responsive to abscisic acid and gibberellin in rice: phenotyping and comparative analysis between rice and Arabidopsis. Physiol Genomics 17:87–100

Uwagi

Rekord w opracowaniu

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-98bc21df-3eb4-40b2-ad04-6b030e2d7490
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.