Prolonged elevation of corticosterone level enhances spontaneous glutamate release in the rat motor cortex

• Autorzy: Czerw A. , Kula J. , Tylko G. , Blasik A. , Hess G.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: Repeated and chronic forms of stress constitute a major risk factor in serious pathologies including depression, cognitive impairments and motor control dysfunctions. However, the mechanisms of the influence of prolonged elevation of the level of corticosteroid hormones on synaptic transmission in the cerebral cortex remain poorly understood. METHODS: We studied the effects of the treatment of male Wistar rats with corticosterone for 7 days on excitatory and inhibitory synaptic inputs as well as on the excitability of layer II/ III pyramidal neurons of the rat motor cortex. Spontaneous excitatory and inhibitory postsynaptic currents (sEPSCs and sIPSCs) were recorded from pyramidal cells in ex vivo slices of the rat frontal cortex, prepared 2 days after the last administration of the hormone. RESULTS: Corticosterone treatment induced an increase in the frequency but not the amplitude of sEPSCs. Measurements with the use of tetrodotoxin (TTX) revealed that most of the recorded sEPSCs represented miniature EPSCs (mEPSCs). The frequency and amplitude of sIPSCs as well as the excitability of pyramidal cells remained unchanged. Corticosterone treatment modified neither the density of dendritic spines on pyramidal neurons nor the protein density levels of selected subunits of AMPA, NMDA and GABAA receptors. CONCLUSIONS: Thus, prolonged administration of exogenous corticosterone selectively enhances glutamatergic transmission in the rat motor cortex, most likely via an enhancement of spontaneous glutamate release from presynaptic terminals. Support: National Science Centre, Poland, grant UMO-2012/07/B/ NZ4/01669

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S46-S47

Twórcy

autor

Czerw A.

• Institute of Zoology, Jagiellonian University, Krakow, Poland

autor

Kula J.

• Institute of Zoology, Jagiellonian University, Krakow, Poland

autor

Tylko G.

• Institute of Zoology, Jagiellonian University, Krakow, Poland

autor

Blasik A.

• Department of Physiology, Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland

autor

Hess G.

• Institute of Zoology, Jagiellonian University, Krakow, Poland
• Department of Physiology, Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland