The use of RAPD markers for detecting genetic similarity and molecular identification of chamomile (Chamomilla recutita (L.) Rausch.) genotypes

• Autorzy: Okon S. , Surmacz-Magdziak A.

Warianty tytułu

PL

Wykorzystanie markerów RAPD do określenia podobieństwa genetycznego oraz molekularnej identyfikacji genotypów rumianku pospolitego (Chamomilla recutita (L.) Rausch)

• Języki publikacji: EN

Abstrakty

EN

The objectives of this study was to assess the of genetic similarity and identification of 13 wild species and 7 cultivars of chamomile using RAPD markers. 53 RAPD primers were screened, only 12 produced polymorphic and repeatable fragments. In total, all primers used produced 157 fragments out of which 149 were polymorphic. The RAPDbased genetic similarity was estimated. Genetic similarity matrix was applied for cluster analysis through UPGM A method. On the dendrogram, only genotypes from Austria, Czech Republic as weel as genotypes collected in area of Lublin were grouped together. The remaining genotypes from the same area were located in different groups. Present study demonstrated that RAPD markers provided a practical and effective method not only to evaluate the genetic similarity and relationships but also to identify chamomile genotypes.

PL

Celem badań było określenie podobieństwa genetycznego oraz identyfikacja 13 dzikich gatunków i 7 odmian rumianku pospolitego za pomocą markerów RAPD. Spośród 53 starterów wybrano 12, które amplifikowały polimorficzne i powtarzalne produkty, łącznie uzyskano 157 fragmentów, z których 149 było polimorficznych. Podobieństwo genetyczne oszacowano w oparciu o markery RAPD. Analizę skupień wykonano metodą średnich połączeń UPGM A. Na uzyskanym dendrogramie tylko genotypy pochodzące z Austrii, Czech oraz zebrane w okolicach Lublina ulegały wspólnemu skupieniu. Reszta analizowanych genotypów pochodzących z tych samych regionów ulegała skupieniu w różnych grupach. Przedstawione badania wykazują, że metoda RAPD jest dobrą metodą nie tylko do oszacowania podobieństwa genetycznego, ale także do identyfikacji genotypów rumianku pospolitego.

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2011
• Tom: 57
• Numer: 1
• Opis fizyczny: p.38-47,fig.,ref.

Twórcy

autor

Okon S.

• Institute of Plant Genetics, Breeding and Biotechnology, University of Life Sciences, Akademicka 15, 20-950 Lublin, Poland

autor

Surmacz-Magdziak A.

Bibliografia

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