Arabidopsis cyclin-dependent kinase gene CDKG;2 is involved in organogenic responses induced in vitro

• Autorzy: Zabicki P. , Kuta E. , Tuleja M. , Rataj K. , Malec P.
• Języki publikacji: EN

Abstrakty

EN

The Arabidopsis CDKG;2 gene encodes a putative cyclin-dependent Ser/Thr protein kinase of unknown biological function. This gene shows structural similarity to animal and human cyclin-dependent (PITSLRE) kinases. This study used the homozygous knockout cdkg;2 mutant based on T-DNA insertional line SALK_090262 to study the effect of mutation of the CDKG;2 gene on explant response and in vitro plant regeneration. For callus induction and proliferation, hypocotyls and cotyledons of 3-day-old seedlings of cdkg;2 and A. thaliana ecotype Col-0 were cultured on solid MS medium supplemented with 2,4-D (2 mg l-1). Organogenesis was induced after callus transfer on MS + TDZ (0.5 mg l-1). The initiation time of callus and shoot induction differed between the mutant and control cultures. Shoot regeneration after callus transfer on MS + TDZ was delayed in cdkg;2 (31 days versus 7 days in Col- 0). Shoots formed on callus derived from Col-0 hypocotyls but not on cotyledon-derived callus; in cdkg;2, shoots developed on both callus types. Mutant shoots did not form roots, regenerants were dwarfed, and inflorescences had small bud-like flowers with a reduced corolla and generative organs. Abnormalities observed during cdkg;2 organogenesis suggest a role of CDKG;2 as a regulator of adventitious root initiation.

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2013
• Tom: 55
• Numer: 1
• Opis fizyczny: p.37-48,fig.,ref.

Twórcy

autor

Zabicki P.

• Department of Plant Cytology and Embryology, Jagiellonian University, Grodzka 52, 31-044 Cracow, Poland

autor

Kuta E.

• Department of Plant Cytology and Embryology, Jagiellonian University, Grodzka 52, 31-044 Cracow, Poland

autor

Tuleja M.

• Department of Plant Cytology and Embryology, Jagiellonian University, Grodzka 52, 31-044 Cracow, Poland

autor

Rataj K.

• Department of Plant Physiology and Biochemistry, Jagiellonian University, Gronostajowa 7, 30-387 Cracow, Poland

autor

Malec P.

• Department of Plant Physiology and Biochemistry, Jagiellonian University, Gronostajowa 7, 30-387 Cracow, Poland

Bibliografia

• ALONSO JM, STEPANOVA AN, LEISSE TJ, KIM CJ et al. 2003. Genome-wide insertional mutagenesis of Arabidopsisthaliana. Science 301: 653–657.
• BASTER P, LEDWOŃ A, GLIWICKA M, TROJANOWSKA A, and GAJ MD. 2009. Arabidopsis tanmei/emb2757 embryo mutant isdefective for in vitro plant morphogenesis. Plant Cell,Tissue and Organ Culture 99: 305–312.
• BOUDOLF V, VLIEGHE K, BEEMSTER GTS, MAGYAR Z, TORRES ACOSTA JA, MAES S, VAN DER SCHUEREN E, INZÉ D, and
• DE VEYLDER L. 2004. The plant-specific cyclin-dependent kinase CDKB1;1 and transition factor E2Fa-DPa control the balance of mitotically dividing and endoreduplicatingcells in Arabidopsis. Plant Cell 16: 2683–2692.
• BOWMAN JL, SMYTH DR, and MEYEROWITZ EM. 2012. The ABC model of flower development: then and now.Development 139: 4095–4098. doi:10.1242/dev.083972.
• BAUS AD, FRANZMAN L, and MEINKE DW. 1986. Growth in vitro of arrested embryos from lethal mutants of Arabidopsisthaliana. Theoretical and Applied Genetics 72: 577–586. CATTEROU M, DUBOIS F, SMETS R, VANIET S, KICHEY T, VAN
• ONCKELEN H, SANGWAN-NORREEL BS, and SANGWAN RS. 2002. hoc: an Arabidopsis mutant overproducing cytokinins and expressing high in vitro organogenic capacity. The Plant Journal 30 (3): 273–287.
• CHANDLER JW. 2008. Cotyledon organogenesis. Journal of Experimental Botany 59: 2917–2931.
• DEWITTE W, and MURRAY JAH. 2003. The plant cell cycle. Annual Review of Plant Biology 54: 235–64.
• DISSMEYER N, NOWACK MK, PUSCH S, STALS H, INZE D, GRINI PE, and SCHNITTGER A. 2007. T-Loop phosphorylation of Arabidopsis CDKA;1 is required for its function and can be partially substituted by an aspartate residue. The Plant Cell 19: 972–985.
• ENUGUTTI B, KIRCHHELLE C, and SCHNEITZ K. 2012. On the genetic control of planar growth during tissue morphogenesisin plants. Protoplasma DOI 10.1007/s00709-012-0452-0.
• FRAŚ A, SMOLEŃ B, and MAŁUSZYŃSKA J. 2008. Vascularization of zygotic and somatic embryos of Arabidopsis thaliana.Acta Biologica Cracoviensia Series Botanica 50 (2):43–48.
• FRIML J. 2003. Auxin transport – shaping the plant. Current Opinion in Plant Biology 6: 7–12.
• GAJ MD. 2001a. Somatyczna Embriogeneza w Kulturach In Vitro Arabidopsis thaliana (L.) Heynh. WydawnictwoUniwersytetu Śląskiego, Katowice.
• GAJ MD. 2001b. Direct somatic embryogenesis as a rapid and fficient system for in vitro regeneration of Arabidopsisthaliana. Plant Cell, Tissue and Organ Culture 64:39–46.
• GAJ MD. 2004. Factors influencing somatic embryogenesis induction and plant regeneration with particular reference to Arabidopsis thaliana (L.) Heynh. Plant GrowthRegulation 43: 27–47.
• GAJ MD, ZHANG S, HARADA JJ, and LEMAUX PG. 2005. Leafy cotyledon genes are essentials for induction of somaticembryogenesis of Arabidopsis. Planta 222: 977–988.
• GAJ MD, TROJANOWSKA A, UJCZAK A, MĘDREK M, KOZIOŁ A, and GARBACIAK B. 2006. Hormone-response mutants ofArabidopsis thaliana (L.) Heynh. impaired in somaticembryogenesis. Plant Growth Regulation 49: 183–197.
• GAUT B. 2012. Arabidopsis thaliana as a model for the genetics of local adaptation. Nature Genetics 44:115–116.
• JACH M, and PRZYWARA L. 2000. Somatic embryogenesis and organogenesis induced on the immature zygotic embryosof Helianthus annuus L. – the role of genotype. ActaBiologica Cracoviensia Series Botanica 42 (2): 83–86.
• JOSHI HJ, CHRISTIANSEN KM, FITZ J, CAO J, LIPZEN A, MARTIN J, SMITH-MORITZ AM, PENNACCHIO LA, SCHACKWITZ WS,WEIGEL D, and HEAZLEWOOD JL. 2012. 1001 Proteomes: afunctional proteomics portal for the analysis ofArabidopsis thaliana accessions. Bioinformatics 28(10):1303–1306.
• GONZÁLEZ GA, PACHECO MG, ONETO CD, ETCHART VJ. KANDUS MV, SALERNO JC et al. 2012. Somatic embryogenesis and plantregeneration capacity in Argentinean maize (Zea mays L.)inbred lines. Electronic Journal of Biotechnology 15(1).DOI: 10.2225/vol15-issue1-fulltext-7
• HEMERLY A, DE ALMEIDA ENGLER J, BERGOUNIOUX C, VAN MONTAGU M, ENGLER G, INZÉ D, and FERREIRA P. 1995. Dominant negative mutants of the CDC2 kinase uncouple cell division from iterative plant development. EMBO Journal 14: 3925–3936.
• INZÉ D, and DE VEYLDER L. 2006. Cell cycle regulation in plant development. Annual Review Genetics 40: 77–105.
• KAPUSTA M, ROJEK J, and BOHDANOWICZ J. 2007. Induction of autonomous endosperm development in ovules of unpollinatedpistils of Arabidopsis thaliana var. Landsbergcultured in vitro. Acta Biologica Cracoviensia SeriesBotanica 49 (2): 53–59.
• KRIKORIAN AD. 1995. Hormones in tissue culture and micropropagation. In: Davies PJ [ed.], Plant Hormones,774–796. Dordrecht: Kluwer.KNOCKAERT M et al. 2002. Pharmacological inhibitors of cyclindependent kinases. Trends in Pharmacological Sciences23: 417–424.
• KONIECZNY R, PILARSKA M, TULEJA M, SALAJ T, and ILNICKI T. 2010. Somatic embryogenesis and plant regeneration inzygotic embryos of Trifolium nigrescens (Viv.). PlantCell, Tissue and Organ Culture 100: 123–130.
• KRAUT M, WÓJCIKOWSKA B, LEDWOŃ A, and GAJ MD. 2011. Immature zygotic embryo cultures of Arabidopis a modelsystem for molecular studies on morphogenic pathwaysinduced in vitro. Acta Biologica Cracoviensia SeriesBotanica 53 (2): 59–67.
• KURCZYŃSKA EU, GAJ MD, UJCZAK A, and MAZUR E. 2007. Histological analysis of direct somatic embryogenesis in Arabidopsis thaliana (L.) Heynh. Planta 226: 619–628
• KUTA E, MALEC P, PILARSKA M, SŁOMKA A, RATAJ K, and ILNICKI T. 2008. Developmental biology of Arabidopsis thaliana At1g67580 mutant. Abstracts of the XXVIII Conference on Embryology: Plants, Animals, Humans, Wisła, May14–17, 2008. Acta Biologica Cracoviensia Series Botanica 50 suppl. 1: 51.
• LAI KS, YUSOFF K, and MAZIAH M. 2011. Extracellular matrix as the early structural marker for Centella asiaticaembryogenic tissues. Plant Biology 55: 549–553.
• LIU K-H, MCCORMACK M, and JEN SHEEN J. 2012. Targeted parallel sequencing of large genetically defined genomicregions for identifying mutations in Arabidopsis PlantMethods 8: 12 http://www.plantmethods.com/content/8/1/12.
• MASSON J, and PASZKOWSKI J. 1992. The culture response of Arabidopsis thaliana protoplasts is determined by the growth conditions of donor plants. The Plant Journal 2:829–833.
• MENGES M, DE JAGER SM, GRUISSEM W, and MURRAY JAH. 2005. Global analysis of the core cell cycle regulators of Arabidopsis identifies novel genes, reveals multiple and highly specific profiles of expression and provides a coherent model for plant cell cycle control. Plant Journal41: 546–566.
• MURASHIGE T, SKOOG F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures.Physiologia Plantarum 15: 473–497
• NAMETH B, DINKA SJ, CHATFIELD SP, MORRIS A, ENGLISH J, LEWIS D, ORO R, and RAIZADA MN. 2013. The shoot regenerationcapacity of excised Arabidopsis cotyledons is establishedduring the initial hours after injury and is modulated bya complex genetic network of light signalling. Plant CellEnvironment 36(1): 68–86.
• NEGRUTIU I, BEEFTINK F, and JACOBS M. 1975. Arabidopsis thaliana as a model system in somatic cell genetics I.Cell and tissue culture. Plant Science Letters 5:293–304.
• NOWAK K, WOJCIKOWSKA B, SZYRAJEW K, and GAJ MD. 2012. Evaluation of different embryogenic systems for productionof true somatic embryos in Arabidopsis. BiologiaPlantarum 56 (3): 401–408.
• PETRICKA JJ, WINTER CM, and BENFEY PN. 2012. Control of Arabidopsis root development Annual Review of PlantBiology. 63: 563–590. DOI: 10.1146/annurev-arplant-042811-105501.
• POPIELARSKA-KONIECZNA M, STARNAWSKA E, and BOHDANOWICZ J. 2010. Extracellular matrix of plant callus tissue visualizedby ESEM and SEM. Protoplasma 247: 121–125.
• ROJEK J, KUTA E, and BOHDANOWICZ J. 2005. In vitro culture promotes partial autonomous endosperm developmentin unfertilized ovules of wild-type Arabidopsis thalianavar. Columbia. Sexual Plant Reproduction 18 (1):29–36.
• ŠAMAJ J, BOBÁK M, BLEHOVÁ A, and PRET'OVÁ A. 2006. Importance of cytoskeleton and cell wall in somaticembryogenesis. In: Mujib A, Šamaj J [eds.], SomaticEmbryogenesis. Springer, Berlin.
• SKOOG F, and MILLER CO. 1957. Chemical regulation of growth and organ formation in plant tissues cultured in vitro.Symposia of the Society for Experimental Biology 11:118–131.
• SEKI M, CARNINCI P et al. 1998, High-efficiency cloning of Arabidopsis full-length cDNA by biotinylated CAP trapper.Plant Journal 15 (5): 707–720.
• SEKI M, NARUSAKA M et al. 2002. Functional annotation of a full-length Arabidopsis cDNA collection. Science 296(5565): 141–145.
• ŚLESAK H, POPIELARSKA M, and GÓRALSKI G. 2005. Morphological and histological aspects of 2,4-D effectson rape explants (Brassica napus L. cv. Kana) culturedin vitro. Acta Biologica Cracoviensia Series Botanica 47(1): 219–226.
• SWARBRECK D, WILKS C, LAMESCH P, BERARDINI TZ, GARCIA-HERNANDEZ M, FOERSTER H, LI D, MEYER T, MULLER R, PLOETZ L, RADENBAUGH A, SINGH S, SWING V, TISSIER C, ZHANG P, HUALA E. 2008. The Arabidopsis Information Resource (TAIR): gene structure and function annotation. NucleicAcids Research 36: D1009–D1014.
• TANK JG, and THAKER VS. 2011 Cyclin dependent kinases and their role in regulation of plant cell cycle. BiologiaPlantarum 55: 201–212.
• TAKATSUKA H, OHNO R, and UMEDA M. 2009. The Arabidopsis cyclin-dependent kinase-activating kinase CDKF;1 is amajor regulator of cell proliferation and cell expansionbut is dispensable for CDKA activation. Plant Journal59: 475–487.
• TREMBLEY JH et al. 2004. Cyclin Dependent Kinase 11 in RNA transcription and splicing. Progress in Nucleic AcidResearch and Molecular Biology 77: 263–288.
• QIAO M, ZHAO ZJ, and XIANG FN. 2012. Arabidopsis thaliana in vitro shoot regeneration is impaired by silencing of IR1. Biologia Plantarum 56 (3): 409–414.
• WOODWARD AW, and BARTEL B. 2005. Auxin: regulation, action, and interaction. Annals of Botany 95: 707–735.
• YANDELL M, and ENCE D. 2012. A beginner's guide to eukaryotic genome annotation. Nature Reviews Genetics 13:329–342. doi:10.1038/nrg3174.
• YANG L, LI Y, and SHEN H. 2012. Somatic embryogenesis and plant regeneration from immature zygotic embryo culturesof mountain ash (Sorbus pohuashanensis). PlantCell Tissue Organ Culture 109: 547–556.
• YOUNG JC, KRYSAN PJ, and SUSSMAN MR. 2001. Efficient screening of Arabidopsis T-DNA insertion lines usingdegenerate primers. Plant Physiology 125: 513–518

Uwagi

PL

Rekord w opracowaniu