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2012 | 30 | 1 |

Tytuł artykułu

Polymorphism in exon 2 of INHBB gene and its relationship with litter size in Jining Grey goats

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The inhibin βB (INHBB) gene was studied as a candidate gene for the prolificacy of Jining Grey goats. According to the sequence of bovine INHBB gene, two pairs of primers were designed to detect single nucleotide polymorphism in exon 2 of INHBB gene in high-prolificacy (Jining Grey)and low-prolificacy (Inner Mongolia Cashmere and Angora) goats by polymerase chain reactionsingle strand conformation polymorphism method. Only the amplified products of primer P2 showed polymorphism. Three genotypes (AA, AB and BB) were identified in Jining Grey and two (AB and BB) in Inner Mongolia Cashmere and Angora goats. Sequencing revealed one single nucleotide mutation (A→G) at base 782 of exon 2 of INHBB gene in BB genotype compared to genotype AA.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

30

Numer

1

Opis fizyczny

p.57-63,fig.,ref.

Twórcy

autor
  • Key Laboratory of Farm Animal Genetic Resources and Utilization of the Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193 Bejing, China
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Bibliografia

  • Goldammer T., Brunner R.M., Hiendleder S., Schwerin M., 1995 − Comparative mapping of sheep inhibin subunits α (INHA) and βB (INHBB) to chromosome 2 in goat by FISH.Mammalian Genome 6, 685-686.
  • Jaeger C., Hiendleder S., 1994 − Cosmid cloning and characterization of the coding regions and regulatory elements of the ovine α-(INHA), βA-(INHBA) and βB-inhibin (INHBB) genes. Animal Genetics 25, Supplement 2, p. 33.
  • LING N., YING S.Y., UENO N., ESCH F., DENOROY L., GUILLEMIN R., 1985 − Isolation and partial characterization of a Mr 32,000 protein with inhibin activity from porcine follicular fluid.Proceedings of the National Academy of Sciences of the United States of America 82, 7217-7221.
  • MASON A.J., HAYFLICK J.S., LING N., ESCH F., UENO N., YING S.Y., GUILLEMIN R, NIALL H., SEEBURG P.H., 1985 − Complementary DNA sequences of ovarian follicular fluid inhibin show precursor structure and homology with transforming growth factor-β. Nature 318, 659-663.
  • MCNATTY K.P., HEATH D.A., HUDSON N.L., BALL K., CONDELL L., 1992 − Concentrations of immunoreactive inhibin in ovarian and peripheral venous plasma and follicular fluid of Booroola ewes that are homozygous carriers or non-carriers of the FecB gene. Journal of Reproduction and Fertility 95, 489-502.
  • PHILLIPS D.J., 2005 − Activins, inhibins and follistatins in the large domestic species. DomesticAnimal Endocrinology 28, 1-16.
  • RIVIER J., SPIESS J., MCCLINTOCK R., VAUGHAN J., VALE W., 1985 − Purification and partial characterization of inhibin from porcine follicular fluid. Biochemical and Biophysical Research Communications 133, 120-127.
  • ROBERTS A.J., REEVES J.J., 1988 − Kidding rates of Angora goats passively immunized against estrogens. Journal of Animal Science 66, 2443-2447.
  • ROBERTSON D.M., FOULDS L.M., LEVERSHA L., MORGAN F.J., HEARN M.T., BURGER H.G. WETTENHALL R.E., de KRETSER D.M., 1985 − Isolation of inhibin from bovine follicular fluid. Biochemical and Biophysical Research Communications 126, 220-226.
  • RODGERS, R.J., STUCHBERY, S.J., FINDLAY, J.K., 1989 − Inhibin mRNAs in ovine and bosine ovarian follicles and corpora lutea throughout the estrous cycle and gestation. Molecular Cell Endocrinology 62, 95-101.
  • SAMBROOK, J., RUSSELL, D.W., 2001 − Molecular Cloning − A Laboratory Manual, 3rd edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
  • THOMPSON, D.A., CRONIN, C.N., MARTIN, F., 1994 − Genomic cloning and sequence analyses of the bovine α-, βA- and βB-inhibin/activin genes. Identification of transcription factor AP-2-binding sites in the 5’-flanking regions by DNase I footprinting. European Journal of Biochemistry 226,751-764.
  • TU, Y.R., 1989 − The Sheep and Goat Breeds in China, pp 88-90, 98-101, Shanghai Science and Technology Press, Shanghai, P.R. China. (in Chinese).
  • VASSALLI, A., MATZUK, M.M., GARDNER, H.A., LEE, K.F., JAENISCH, R., 1994 − Activin/inhibin beta B subunit gene disruption leads to defects in eyelid development and female reproduction.Genes and Development. 8, 414-427.
  • WOODRUFF T.K., BESECKE, L.M., GROOME, N., DRAPER, L.B., SCHWARTZ, N.B., WEISS,J., 1996 − Inhibin A and inhibin B are inversely correlated to follicle-stimulating hormone, yet are discordant during the follicular phase of the rat estrous cycle, and inhibin A is expressed in a sexually dimorphic manner. Endocrinology 137, 5463-5467.
  • Xue , Y., Chu , M.X., Zhou , Z.X., 2004 − Advances on inhibin genes. Hereditas (Beijing). 26,749-755 (in Chinese, summary in English).

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Bibliografia

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