Antibiotic susceptibility and genotype patterns of Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa isolated from urinary tract infected patients

• Autorzy: Abou-Dobara M.I. , Deyab M.A. , Elsawy E.M. , Mohamed H.H.
• Języki publikacji: EN

Abstrakty

EN

Thirty nine isolates of Escherichia coli, twenty two isolates of Klebsiella pneumoniae and sixteen isolates of Pseudomonas aeruginosa isolated from urinary tract infected patients were analyzed by antimicrobial susceptibility typing and random amplified polymorphic DNA (RAPD)-PCR. Antibiotic susceptibility testing was carried out by microdilution and E Test methods. From the antibiotic susceptibility, ten patterns were recorded (four for E. coli, three for K. pneumoniae and three for P. aeruginosa respectively). Furthermore, genotyping showed seventeen RAPD patterns (seven for E. coli, five for K. pneumoniae and five for P. aeruginosa respectively). In this study, differentiation of strains of E. coli, K. pneumoniae and P. aeruginosa from nosocomial infection was possible with the use of RAPD.

Słowa kluczowe

EN

antibiotic susceptibility; genotype; Escherichia coli; Klebsiella pneumoniae; Pseudomonas aeruginosa; isolation; urinary tract; human infection; patient; human disease; urinary tract disease

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2010
• Tom: 59
• Numer: 3
• Opis fizyczny: p.207-212,fig.,ref.

Twórcy

autor

Abou-Dobara M.I.

• Faculty of Science (Damietta), Damietta Branch, Mansoura University, Egypt

autor

Deyab M.A.

• Faculty of Science (Damietta), Damietta Branch, Mansoura University, Egypt

autor

Elsawy E.M.

• Urology and Nephrology Center, Mansoura University, Egypt

autor

Mohamed H.H.

• Urology and Nephrology Center, Mansoura University, Egypt

Bibliografia

• Belkum A. 1994. DNA fingerprinting of medically important microorganisms by use of PCR. Clin. Micribiol. Rev. 7: 174-184.
• Bingen E.H., C. Boissinot, P. Desjardins, H. Cave, N. Brahimi, N. Lambert-Zechovsky, E. Denamur, P. Blot and J. Elion. 1993a. Arbitrarily primed polymerase chain reaction provides rapid differentiation of Proteus mirabilis isolates from a pediatric hospital. J. Clin. Microbiol. 31: 1055-1059.
• Bingen E.H., H. Cave, Y. Aujard, N. Lambert-Zechovsky, J. Elion and E. Denamur. 1993b. Molecular analysis of multiply recurrent meningitis due to Escherichia coli K1 in an infant. Clin. Infect. Dis. 16: 82-85.
• Bosi C, A. Davin-Regli, C. Bornet, M. Mallea, J. Pages and C. Bollet. 1999. Most Enterobacter aerogenes strains in France belong to a prevalent clone. J. Clin. Microbiol. 37:2165-2169.
• Brenner D.J. 1986. Family I. Enterobacteriaceae. In: Bergey's Manual of Systematic Bacteriology (Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T. and Williams, S.T. (eds). Williams and Wilkins, Baltimore.
• Centers for Disease Control (CDC). 1983. Nosocomial infection surveillance 1980 1982. CDC Surveillance Summaries, 32 (455): 155.
• Chit L.P. and C.Y. Chew. 1993. Recent advances in the typing of Pseudomonas aeruginosa. J. Hosp. Infect. 24: 175-181.
• Das R.N, T.S. Chandrashekhar, H.S. Joshi, M. Gurung, N. Shrestha and P.G. Shivananda. 2006. Frequency and susceptibility profile of pathogens causing urinary tract infections at a tertiary care hospital in western Nepal. Singapore Med. J. 47(4): 281-285.
• Eisen D., E.G. Russeli, M. Tymms, E.J. Roper, M.L. Grayson and J. Turnidge. 1995. Random Amplified Polymorphic DNA and Plasmid Analyses Used in Investigation of an Outbreak of Multiresistant Klebsiella pneumoniae. J. Clin. Microbiol. 33: 713-717.
• Ellsworth D.L., K.D. Rittenhouse and R.L. Honeycutt. 1993. Artifactual variation in randomly amplified polymorphic DNA banding patterns. Bio-Techniques 14: 214-217.
• Foda A. 2008. Ph.D. Thesis. Zagazig University. Zagazig. Egypt. Gales A.C., R.N. Jones, K.A. Gordon, H.S. Soder, W.W. Wike, M.L. Beach, M.A., Pfaller, M.V. Doern and the SENTRY Study Group (Latin America). 2000. Activity and spectrum of 22 antimicrobial agent tested against urinary tract infection pathogens in hospitalized patients in Latin America: reported from the second year of the SENTRY Antimicrobial Surveillance Program (1998). J. Antimicrob. Microb. Chemotherapy 45: 295-303.
• Gomes A.R., L. Muniyappa, G. Krishnappa, V.V.S. Suryanaryna, S. Isloor, B. Parakash and P.G. Hugar. 2005. Genotypie Characterization of Avian Escherichia coli by Random Amplification of Polymorphic DNA. International J. Poultry Science 3: 378-381.
• Gori A., F. Espinasse, A. Deplano, C. Nonhoff, M.H. Nicolas and M.J. Struelens. 1996. Comparison of PFGE and RAPD for typing extended-spectrum beta-lactamase producing Klebsiella pneumoniae. J. Clin. Microbiol. 10: 2448-2453.
• Gupta N., S. Kundra, A. Sharma and V. Gautam. 2007. Antimicrobial susceptibility of uropathogens in India. J. Infect. Dis. Antimicrob. Agents. 24: 13-18.
• Hilton A.C. and C.W. Penn. 1998. Comparison of ribotyping and arbitrarily primed PCR for molecular typing of Salmonella enterica and relationships between strains on the basis of these molecular markers. J. Appl. Microbiol. 85: 933-940.
• Horrevorts A.M., J. Borst and R.J. Puyk. 1990. Ecology of Pseudomonas aeruginosa in patient with cystic fibrosis. J. Med. Microbial. 31: 119-124.
• Khalifa A. B., H. Vu-Thien, C. Pourcel, M. Khedher, M. Mastouri and D. Moissenet. 2010. Phenotypic and genotypie (randomly amplified polymorphic analysis and multiple locus variable-number tandem-repeat analysis) characterization of 96 clinical isolates of Pseudomonas aeruginosa in the F. Bourguiba (Monastir, Tunisia). Pathologie Biologie 58: 84-88.
• Kerr J.R., J.E. Moore, M.D. Curran, R. Grahm, C.H. Webb, K.G. Lowry, P.G. Murphy, T.S. Wilson and W.P. Ferguson. Investigation of a nosocomial outbreak of Pseudomonas aeruginosa pneumonia in an intensive care unit by random amplification of polymorphic DNA assay. J. Hospital Infection. 30: 125-131.
• Lai Y.C., S.L. Yang, H.L. Peng and H.Y. Chang. 2000. Identification of genes present specifically in a virulent strain of Klebsiella pneumoniae. Infect. Control. 68: 7149-7151.
• Lin A.W., M.A. Usera, T.J. Barrettand and R.A. Goldsby. Amplification of random amplified polymorphic DNA analysis to differentiate strains of Salmonella enteritidis. J. Clin. Microbial. 34: 870-876.
• Lopes, A.C., J.F. Rodrigues and M. A. Júnior. 2005. Molecular typing of Klebsiella pneumoniae isolates form public hospitals in Recife, Brazil. Microbiological Research. 160: 37-46.
• Lübeck P.S. and J. Hoorfar. 2003. PCR technology and applications to zoonotic food-borne bacterial pathogens. In: PCR detection of microbial pathogens (Methods in molecular biology) Volume 216, (Sachse, K. and Frey, J. (Editors), Humana press.
• Maniatis T., E.F. Fritsch and J. Sambrook. 1982. Molecular cloning. A laboratory manual. Cold Spring Harbor. New York.
• Muzurier S.I. and K. Wernas. 1992. Typing of Listeria strains by random amplification of polymorphic DNA. Res. Microbiol. 143: 499-505.
• National Committee for Clinical Laboratory Standards (NCCLS). 2006. Interpretation of antibiotic sensitivity according to guidelines of NCCLS.
• Nazik H., B. Ongen, B. Erturan and M. Salcioglu. 2007. Genotype and antibiotic susceptibility patterns of Pseudomonas aeruginosa and Stenotrophomonas maltophila isolated from cystic fibrosis patients. Jpn. J. Infect. Dis. 60: 82-86.
• Orskov I. and F. Orskov. 1984. Serotyping of Klebsiella pneumoniae. Methods Microbiol. 14: 143-164.
• Ørskov F. 1986a. Genus I. Escherichia. In: Bergey's Manual of Systematic Bacteriology (Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T. and Williams, S.T. (eds). Williams and Wilkins, Baltimore.
• Ørskov I. 1986b. Genus V. Klebsiella. In: Bergey's Manual df Systematic Bacteriology (Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J. and Williams, S.T. (eds). Williams and Wilkins, Baltimore.
• Palleroni N.J. 1986. Family I. Pseudomonadaceae. In: Bergey's Manual of systematic Bacteriology (Holt, J.G., Krieg, N.R., Sneath, PH.A., Staley, J.T. and Williams, S.T. (eds). Williams and Wilkins, Baltimore.
• Patton T.G., S. Katz, R.J. Sobieski and S.S. Crupper. 2001. Genotyping of clinical Serratia marcescens isolate: a comparison of PCR-based methods. FEMSMicrobiol. Lett. 194: 19-25.
• Sener B., O. Koseoglu and U. Ozcelik. 2001. Epidemiology of chronic Pseudomonas aeruginosa infectin in cystic fibrosis. Int. J. Med. Microbial. 291: 387-393.
• Trautmann M., C. Bauer, C. Schumann, P. Hahn, M. Höher, M. Haller and P.M. Lepper. 2006. Common RAPD pattern of Pseudomonas aeruginosa from patients and Tap water un a medical intensive care unit. Int. J. Hyg. Environ. Health. 209: 325-331.
• Turck M. and W. Stamm. 1981. Nosocomial infections of the urinary tract. Am. J. Med. 70: 651.
• Welsh J. and M. McClelland. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic acid Res. 18: 7213-7218.
• Williams J.G.K., A. R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531-6535.
• Williams J.G.K., M.K. Hanafey, J.A. Rafalski and S.V. Tingey. 1993. Genetic analysis using random amplified polymorphic DNA markers. Methods Enzymol. 218: 704-744.
• Yuksel S., B. Ozturk, A. Kavaz, Z.B. Ozcakar, B. Acar, H. Guriz, D. Aysev, M., Ekim and F. Yalcinkaya. 2006. Antibiotic resistance or urinary tract pathogen and evaluation of empirical treatment in Turkish children with urinary tract infections. Int. J. Antimicro. Agents. 5: 413-416.