Effect of probiotic strains mixture administration on serum interleukins concentration, lymphocyte proliferation and DNA damage in rams

• Autorzy: Abdelrazek H.M.A. , Yusuf M.S. , Isail S.A. , Elgawish R.A.
• Języki publikacji: EN

Abstrakty

EN

Probiotics are live bacteria that could exert health beneficial effects; however, their immunological effects in sheep are still scarce. The aim of this study was to investigate the immunomodulatory role of probiotics in 12 rams weighing 30–35 kg. The animals were divided into 2 groups (6 animals each), one group served as a control and the other as a probiotic treated one. Treated rams were given orally commercial probiotic strains (Sacharomyces cerevisiae, Lactobacillus acidophilus, Lactobacillus plantarum and Enterococcus faecalis) mixture in a dose of 10 g/head/day for one month. Blood samples were collected from control and treated rams at the end of the whole experimental period. Total and differential leukocyte counts (TLC and DLC), interleukin 10 (IL-10) and 12 (IL-12) serum concentration, IL-10/IL-12 ratio, lymphocyte transformation and DNA damage (comet assay) were determined. TLC in the blood of treated rams decreased significantly (P = 0.009), the serum levels of IL-10 and IL-12 were not significantly changed, while IL-10/IL-12 ratio was significantly (P = 0.03) increased after probiotic mixture administration in rams. The administration of probiotic mixture significantly (P = 0.0001) enhanced the transformation of lymphocytes in rams’ blood. After probiotic mixture administration, the percent of DNA in the comet tail declined significantly (P = 0.02) from 2.75% to 2.57%. The present study demonstrated that leukocytes, cytokine production, lymphocyte transformation and DNA integrity were differentially influenced by the tested probiotic strains mixture. The biological importance of specific effects of probiotics in sheep remains to be determined.

• Wydawca: -
• Czasopismo: Journal of Animal and Feed Sciences
• Rocznik: 2015
• Tom: 24
• Numer: 4
• Opis fizyczny: p.302-307,fig.,ref.

Twórcy

autor

Abdelrazek H.M.A.

• Department of Physiology, Suez Canal University, Faculty of Veterinary Medicine, Ismailia 41522, Egypt

autor

Yusuf M.S.

• Department of Nutrition and Clinical Nutrition, Suez Canal University, Faculty of Veterinary Medicine, Ismailia 41522, Egypt

autor

Isail S.A.

• Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44111, Egypt

autor

Elgawish R.A.

• Department of Forensic Medicine and Toxicology, Suez Canal University, Faculty of Veterinary Medicine, Ismailia 41522, Egypt

Bibliografia

• Babar V., Thomas R., Bhaskar M., 2012. Immunomodulatory activity of Lactobacillus sporogenes. Int. J. Ther. Appl. 3, 32–38
• Bien E., Balcerska A., Adamkiewicz-Drozynska E., Rapala M., Krawczyk M., Stepinski J., 2009. Pre-treatment serum levels of interleukin-10, interleukin-12 and their ratio predict response to therapy and probability of event-free and overall survival in childhood soft tissue sarcomas, Hodgkin’s lymphomas and acute lymphoblastic leukemias. Clin. Biochem. 42, 1144–1157
• Budd R.C., 2002. Death receptors couple to both cell proliferation and apoptosis. J. Clin. Invest. 109, 437–442
• D’Andrea A., Aste-Amezaga M., Valiante N.M., Ma X., Kubin M., Trinchieri G., 1993. Interleukin 10 (IL-10) inhibits human lymphocyte interferon y-production by suppressing natural killer cell stimulatory factor/ IL- 12 synthesis in accessory cells. J. Exp. Med. 178, 1041–1048
• de Moreno de LeBlanc A., Castillo N.A., Perdigón G., 2010. Anti-infective mechanisms induced by a probiotic Lactobacillus strain against Salmonella enterica serovar Typhimurium infection. Int. J. Food Microbiol. 138, 223–231
• Dong H., Rowland I., Parveen Yaqoob P., 2012. Comparative effects of six probiotic strains on immune function in vitro. Brit. J. Nutr. 108, 459–470
• Feldman B.F., Zinki J.G., Jain V.C., 2000. Laboratory techniques for avian hematology. In: Schalm’s Veterinary Hematology. 5th Edition. Lippincott Williams & Wilkins. Toronto (Canada), pp. 1145–1146
• Galdeano C.M., Perdigón G., 2006. The probiotic bacterium Lactobacillus casei induces activation of the gut mucosal immune system through innate immunity. Clin. Vaccine Immunol. 13, 219–226
• Gao S., Wang Y., Zhang P., Dong Y., Li B., 2008. Subacute oral exposure to dibromoacetic acid induced immunotoxicity and apoptosis in the spleen and thymus of the mice. Toxicol. Sci. 105, 331–341
• Guillen L.M., 2009. Determination of the mechanism(s) by which directfed microbials control Escherichia coli O157:H7 in cattle. PhD. Thesis, Oklahoma State University (USA) http://digital.library.okstate.edu/etd/Guillen_okstate_0664D_10201.pdf
• Hamer H.M., Jonkers D.M., Vanhoutvin S.A., Troost F.J., Rijkers G., de Bruïne A., Bast A., Venema K., Brummer R.J., 2010. Effect of butyrate enemas on inflammation and antioxidant status in the colonic mucosa of patients with ulcerative colitis in remission. Clin. Nutr. 29, 738–744
• Hart A.L., Lammers K., Bigidi P., Vitali B., Rizzello F., Gionchetti P., Campieri M., Kamm M.A., Knight S.C., Stagg A.J., 2004. Modulation of human dendritic cell phenotype and function by probiotic bacteria. Gut 53, 1602–1609
• Hessle C., Andersson B., Wold A.E., 2000. Gram-positive bacteria are potent inducers of monocytic interleukin-12 (IL-12) while gram-negative bacteria preferentially stimulate IL-10 production. Infect. Immunol. 68, 3581–3586
• Hua M.C., Lin T.Y., Lai M.W., Kong M.S., Chang H.J., Chen C.C., 2010. Probiotic Bio-three induces Th1 and antiinflammatory effects in PBMC and dendritic cells. World J. Gastroenterol. 16, 3529–3540
• Ishikawa F., Miyazaki S., 2005. A functional role of neutrophils in the regulation of innate and acquired immunity to bacterial infection. Pak. J. Biol. Sci. 8, 940–948
• Ismail S.H., 2013. Effects of probiotics supplementation on some blood indices in Iraq diabetic patients. Pharmacie Globale (IJCP) 4 (8), 1–4
• Kizilian N., Wilkins R.C., Reinhardt P., Ferrarotto C., McLean J.R.N., McNamee J.P., 1999. Silver-stained comet assay for detection of apoptosis. Biotechniques 27, 926–928
• Kuitunen M., 2013. Probiotics and prebiotics in preventing food allergy and eczema. Curr. Opin. Allergy Clin. Immunol. 13, 280–286
• Kunavue N., Lien T.F., 2012. Effects of fulvic acid and probiotic on growth performance, nutrient digestibility, blood parameters and immunity of pigs. J. Anim. Sci. Adv. 2, 711–721
• Lebeer S., Vanderleyden J., De Keersmaecker S.C., 2008. Genes and molecules of lactobacilli supporting probiotic action. Microbiol. Mol. Biol. Rev. 72, 728–764
• Lee J., Yun H.S., Cho K.W., Oh S., Kim S.H., Chun T., Kim B., Whang K.Y., 2011. Evaluation of probiotic characteristics of newly isolated Lactobacillus spp.: Immune modulation and longevity. Int. J. Food Microbiol. 148, 80–86
• Lopez P., Gueimonde M., Margolles A., Suárez A., 2010. Distinct Bifidobacterium strains drive different immune responses in vitro. Int. J. Food Microbiol. 138, 157–165
• Medina M., Izquierdo E., Ennahar S., Sanz Y., 2007. Differential immunomodulatory properties of Bifidobacterium logum strains: relevance to probiotic selection and clinical applications. Clin. Exp. Immunol. 150, 531–538
• Mileti E., Matteoli G., Iliev I.D., Rescigno M., 2009. Comparison of the immunomodulatory properties of three probiotic strains of Lactobacilli using complex culture systems: prediction for in vivo efficacy. PLoS One 4 (9), e7056
• Nicole C., Dan D., 2010. Probiotics identification and ways of action. Innov. Rom. Food Biotechnol. 6, 1–11
• Osman M.A., 2012. Modification of the Animal Immune System by Feeding Probiotics. Graduate Theses and Dissertations. Paper, 12424. Iowa State University, Iowa (USA)
• Rao K.S., 2009. Free radical induced oxidative damage to DNA: relation to brain aging and neurological disorders. Indian J. Biochem. Biophys. 46, 9–15
• Vizoso Pinto M.G., Rodriguez Gomez M., Seifert S., Watzl B., Holzapfel W.H., Franz C.M., 2009. Lactobacilli stimulate the innate immune response and modulate the TLR expression of HT29 intestinal epithelial cells in vitro. Int. J. Food Microbiol. 133, 86–93

Identyfikatory

DOI

10.22358/jafs/65612/2015