Transcriptomic landscape of Dendrobium huoshanense and its genes related to polysaccharide biosynthesis

• Autorzy: Han R. , Xie D. , Tong X. , Zhang W. , Liu G. , Peng D. , Yu N.
• Języki publikacji: EN

Abstrakty

EN

Dendrobium huoshanense has long been used to treat various diseases in oriental medicine. In order to study its gene expression profile, transcripts involved in the biosynthesis of precursors of polysaccharides, as well as mechanisms underlining morphological differences between wild and cultivated plants, three organs of both wild type and cultivated D. huoshanense were collected and sequenced by Illumina HiSeq4000 platform, yielding 919,409,540 raw reads in FASTQ format. After Trinity de novo assembly and quality control, 241,242 nonredundant contigs with the average length of 967.5 bp were generated. qRT-PCR experiment on the selected transcripts showed the transcriptomic data were reliable. BLASTx was conducted against NR, SwissProt, String, Pfam, and KEGG. Gene ontology annotation revealed more than 40,000 contigs assigned to catalytic activity and metabolic process, suggesting its dynamic physiological activities. By searching KEGG pathway, six genes potentially involved in mannose biosynthetic pathway were retrieved. Gene expression analysis for stems between wild and cultivated D. huoshanense resulted in 956 genes differentially expressed. Simple sequence repeats (SSRs) analysis revealed 143 SSRs with the unit size of 4 and 3,437 SSRs the size of 3. The obtained SSRs are the potential molecular markers for discriminating distinct cultivars of D. huoshanense.

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2018
• Tom: 87
• Numer: 1
• Opis fizyczny: Article 3574 [11p.],fig.,ref.

Twórcy

autor

Han R.

• School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
• Anhui Innovative Team from Colleges for Scientific Research’s Platform – The Innovative Team in Researching the Key Technologies Concerning the Integration of Processing Chinese Medicine Decoction Pieces in Producing Area, Hefei 230012, China

autor

Xie D.

• School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China

autor

Tong X.

• Institute of Metabolic and Cardiovascular Diseases, Joint Research Unit 1048 INSERM Adipolab Unit, Paul Sabatier University, Toulouse 31432, France

autor

Zhang W.

• School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China

autor

Liu G.

• School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China

autor

Peng D.

• School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China

autor

Yu N.

• School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China

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