In vitro techniques used in breeding program in the Lithuanian Institute of Horticulture

• Autorzy: Rugienius R. , Stanys V. , Staniene G. , Shikshnianas T. , Gelvonauskiene D. , Gelvonauskis B.

Warianty tytułu

PL

In vitro techniki stosowane w programie hodowli roślin sadowniczych w Litewskim Instytucie Ogrodnictwa

• Języki publikacji: PL

Abstrakty

PL

Przydatność oraz wydajność różnych technik in vitro (mikrorozmnażanie, krzyżowanie oddalone z wykorzystaniem techniki izolowanych zarodków i selekcja in vitro roślin z pożądanymi cechami) w programie hodowli roślin sadowniczych została wykazana w oparciu o wyniki badań realizowanych w Zakładzie Genetyki i Biotechnologii Roślin Instytutu Ogrodnictwa w Kownie. W zoptymalizowanych warunkach wydajność mikrorozmnażania śliwy wynosiła od 3 do 6 pędów z eksplantatu. W mikrorozmnażaniu Chaenomeles japonica najwyższy stopień ukorzeniania osiągnięto przy pH pożywki niższym niż 5,0. Najważniejszym czynnikiem dla wydajności techniki embryo rescue okazał się termin izolacji zarodków (43 dzień po zapyleniu). Wyniki selekcji in vitro roślin z pożądanymi cechami zostały potwierdzone w 20 do 100% przez wyniki uzyskane w warunkach in vivo (zależnie od poly- lub monogenicznego charakteru cechy).

EN

Usefulness and efficiency of different in vitro techniques (micropropagation, distant hybridization with using embryo rescue techniques and desired trait screening) in fruit plant breeding program was shown, basing on the results of studies realized in Department of Plant Genetics and Biotechnology of Horticulture Institute in Kaunas. Under the optimized conditions, the efficiency of plum micropropagation was 3-6 shoots per explant. In micropropagation of Japanese quince the highest rooting average (about 43%) was obtained at a medium pH lower than 5.0. The most important factor of embryo rescue efficiency was the time of isolation (43rd day after pollination). Results of in vitro screening of desired plant characters were confirmed by in vivo analysis at the level from 20 to 100%, depended on poly- or monogenic type of trait.

• Wydawca: -
• Czasopismo: Zeszyty Problemowe Postępów Nauk Rolniczych
• Rocznik: 2004
• Tom: 497
• Numer: 2
• Opis fizyczny: p.727-733,fig.,ref.

Twórcy

autor

Rugienius R.

• Department of Plant Genetics and Biotechnology, Lithuanian Institute of Horticulture, LT-4335 Babtai, Kaunas district, Lithuania

autor

Stanys V.

• Department of Plant Genetics and Biotechnology, Lithuanian Institute of Horticulture, LT-4335 Babtai, Kaunas district, Lithuania

autor

Staniene G.

• Department of Plant Genetics and Biotechnology, Lithuanian Institute of Horticulture, LT-4335 Babtai, Kaunas district, Lithuania

autor

Shikshnianas T.

• Department of Plant Genetics and Biotechnology, Lithuanian Institute of Horticulture, LT-4335 Babtai, Kaunas district, Lithuania

autor

Gelvonauskiene D.

• Department of Plant Genetics and Biotechnology, Lithuanian Institute of Horticulture, LT-4335 Babtai, Kaunas district, Lithuania

autor

Gelvonauskis B.

• Department of Plant Genetics and Biotechnology, Lithuanian Institute of Horticulture, LT-4335 Babtai, Kaunas district,Lithuania

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