Antimicrobial and antioxidant activity of Epimedium pinnatum

• Autorzy: Mahboubi M. , Kazempour N. , Hosseini H. , Mahboubi M.

Warianty tytułu

PL

Przeciwbakteryjne i przeciwutleniające działanie Epimedium pinnatum

• Języki publikacji: EN

Abstrakty

EN

Epimedium pinnatum (Berberidaceae family) is used as an aphrodisiac in traditional Chinese medicine (TCM). The aim of this study was to evaluate the antimicrobial and antioxidant activity of E. pinnatum extracts (ethanol, methanol and aqueous extracts). Total phenolic (TPC) and flavonoid contents (TFC) of each extract were assessed by spectrophotometric methods. It was exhibited that methanol extract had better antimicrobial activity than those of ethanolic extract or aqueous extract. The TPC and TFC of E. pinnatum extracts was higher in methanol extract (149 and 36.6 mg/g) than that of ethanolic extract (137.2 and 19.5 mg/g) and aqueous extract (86.2 and 8.4 mg/g). The methanol extract had lower IC50 value (200 µg/ml) than ethanolic (250 µg/ml) and aqueous extract (400 µg/ml). There was a positive correlation between TPC, TFC in E. pinnatum extract and their antioxidant and antimicrobial activity.

PL

W tradycyjnej medycynie chińskiej Epimedium pinnatum (rodzina Berberidaceae) używa się jako afrodyzjaku. Celem niniejszej pracy była ocena działania przeciwbakteryjnego i przeciwutleniającego wyciągów: etanolowego, metanolowego i wodnego z Epimedium pinnatum. Całkowita zawartość fenoli i całkowita zawartość flawonoidów w każdym z wyciągów została oznaczona metodami spektrofotometrycznymi. Wykazano, że wyciąg metanolowy miał lepsze działanie przeciwbakteryjne niż wyciąg etanolowy i wodny. Całkowita zawartość fenoli i całkowita zawartość flawonoidów była wyższa w wyciągu metanolowym (149 i 36,6 mg/g) niż etanolowym (137,2 i 19,5 mg/g) i wodnym (86,2 i 8,4 mg/g). Wyciąg metanolowy miał niższą wartość IC50 (200 µg/ml) niż wyciąg etanolowy (250 µg/ml) i wodny (400 µg/ml). Zauważono pozytywną korelację między całkowitym stężeniem fenoli i całkowitym stężeniem flawonoidów w wyciągach z E. pinnatum, a ich działaniem przeciwutleniającym i przeciwbakteryjnym.

Słowa kluczowe

EN

antimicrobial activity; antioxidant activity; Epimedium pinnatum; medicinal plant; plant extract; phenolics; total phenolics; flavonoids; total flavonoids

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2013
• Tom: 59
• Numer: 2
• Opis fizyczny: p.24-34,fig.,ref.

Twórcy

autor

Mahboubi M.

• Microbiology Group, Medicinal Plants Research Center of Barij Essence, 87135-1178 Kashan, Iran

autor

Kazempour N.

• Microbiology Group, Medicinal Plants Research Center of Barij Essence, 87135-1178 Kashan, Iran

autor

Hosseini H.

• Agricultural Group, Medicinal Plants Research Center of Barij Essence, 87135-1178 Kashan, Iran

autor

Mahboubi M.

• Chemistry Department, Chabahar Free Trade and Industrial Zone Organization, Iran

Bibliografia

• 1. Chen CYC. Computational screening and design of traditional Chinese medicine (TCM) to block phosphodiesterase-5. J Mol Graphics Modell 2009; 28:261-269.
• 2. Chen KK, Chiu JH: Effect of Epimedium brevicornum Maxim. extract on elicitation of penile erection in the rat. J Urol 2006; 67:631-635.
• 3. Wang F, Zheng Y, Xiao HB, Zhou M, Liu YX, Li GQ: Effect of administration of herba Epimedium at the optimal time levels on sexual hormones. J Trad Chin Med 2001; 42:619-621.
• 4. She BR, Qin DN, Wang Z, She YC: Effects of flavonoids from Herba Epimediim on the reproduetive system in male rats. Chin J Androl 2003; 17:294-296.
• 5. Meng FH, Li YB, Xiong ZL, Jiang ZM, Li FM: Osteoblastic proliferative activity of Epimedium brevicornum Maxim. Phytomedicine 2005; 12:189-193.
• 6. Zhang DW, Cheng Y, Zhang JC, Wang XL, Wang NL, Chen Y, Yang MS, Yao XS: Synergistic effect of trace elements and flavonoids from Epimedium koreanum Nakai on primary osteoblasts. Chin Sci Bull 2008; 53:347-356.
• 7. Ding Y, Xin ST, Zhou JH: Effect of Epimedium polysaccharide on the transfer of mice thymus cells. Chin Pharmacol 1992; 9:28-29.
• 8. Hu YL, Lv DH, Liu JG, Song DL: Effect of Epimedium-proplis adjuvant (EPA) on activity of celiac macrophages in chicks and mice. China J Vet Sci Technol 1999; 5:8-11.
• 9. Liang HR, Vuorela P, Vuorela H, Hiltunen R: Isolation and immunomodulatory effect of flavonol glycosides from Epimedium hunanense. Planta Med 1997; 63:316-319.
• 10. Wang JJ, Tang QZ, Wang T, Liu M, Wang XL: Effect of Icariin on L2 type calcium current in isolated ventricular myocytes. Med J Wuhan Univ 2007; 28:282-285.
• 11. Yu L, Li H, Huang G, Bai Y, Dong Y: Clinical observation on treatment of 120 cases of coronary heart disease with Herba Epimedii. J Trad Chin Med 1992; 12,30–34.
• 12. Fu LB, Xia YH, Yu L, Wu J, Zhao H: Empirical study on the effect of the total flavone of herba Epimedium on arterial pressure of rats and its mechanism. Chin J App Physiol 2007; 23:115–116.
• 13. Zhang L, Wang Y, Mao HT, Wen PE, Cai SL, Li XB: Study on the inhibition of telomerase activity and regulated mechanism in human cancer cell by icarrin. Chin J Immunol 2002; 18:191-196.
• 14. Zhao Y, Cui ZY, Zhang L, Li SZ: Effects of Icariin on the differentiation of HL-60 cells. Chin J Radiat Oncol 1997; 19:53-55.
• 15. Mao HT, Zhang L, Wang Y, Li XP, Cui ZY: Studies of suppression on invasion and metastasis ability of high metastatic human lung tumor cell line. Chin J Immunol 2001; 17:27-29.
• 16. Liu XY, Shen ZY, Wu B, Xia SJ, Huang JH, Chen WH, Ying J: Study on changes of protein phosphorylation of p65, Ikappa Balpha and Ikappa Bepsilon in lymphocytes of rats in progress of aging and interventional effect of Epimedium flavonoids. China J Chin Mat Med 2008; 33:73-76.
• 17. Wang XM, Fu H, Liu GX: Effect of Herba Epimedii and Fructus Lyciion mitochondrial DNA deletion, activity of respiratory chain enzyme complexes and ATP synthesis in aged rats. Health Sci 1996; 21:683–685.
• 18. Zhao WH, Deng ZY, Fan QS: Anti-oxidation on total flavones of Epimedium in vitro. J Nanchang Univ (Nat Sci) 2009, 30:53-60.
• 19. Zhang RX, Jia ZP, Li FS, Li MX, Qiu JG: Antihypoxic effect of total flavonoids of Herba Epimedii on hypoxia model mice. J Chin Med Mat 2009; 32:1736-1738.
• 20. Ma JQ, Ma XQ, Zhang XY: Mechanism of resisting exercise fatigue by Epimedium flavones in mice. Chin J New Drugs 2009;18:553-555.
• 21. Palmer DD. The Chiropractor. Beacon Light Printing Company, Los Angeles, CA 1914.
• 22. Fang F, Xu MY, Jiang JJ, Xu YL, Wei HX. Clinical and empirical research of “Chuan Ke Zhi” in treating childhood respiratory viral infection. Shanghai J Trad Chin Med 2003; 37:36-37.
• 23. Yan ZK, Qiu H. Antimicrobial tests of Icariin. China Food Additives 2005; 4:65–68.
• 24. Mozaffarian V. A Dictionary of Iranian Plant Names. Farhang Moaser, Tehran 1996.
• 25. ISO 14502-1. Determination of substances characteristic of green and black tea. Part 1: Content of total polyphenols in tea. Colorimetric method using Folin-Ciocalteu reagent. 2005.
• 26. Woisky RG, Salatino A. Analysis of propolis: some parameters and procedures for chemical quality control. J Apic Res 1998; 37:99-105.
• 27. Kiranmai M, Kumar M, Ibrahim MD: Free radical scavenging activity of neem tree (Azadirachta indica A. Juss Var., Meliaceae) root bark extract. Asian J Pharm Clin Res 2011; 4:134-136.
• 28. Wettasinghe M, Shahidi F: Antioxidant and free radical-scavenging properties of ethanolic extracts of defatted borage (Borago officinalis L.) seeds. Food Chem 1999; 67:399-414.
• 29. Marchetti O, Moreillon P, Glauser M, Bille J, Sanglard D. Potent synergism of the combination of fluconazole and cyclosporine in Candida albicans. Antimicrob Agents Chemother 2000; 44:2373-2381.
• 30. NCCLS. Methods For dilution Antimicrobial susceptibility tests for bacteria that grow aerobically. Approved Standard M7-A7, 7th ed. Pennsylvania 2006.
• 31. Carson C, Hammer K, Riley T. In vitro activity of the essential oil of Melaleuca alternifolia against Streptococcus ssp. J. Antimicrob Chemother 1996; 37:1177-1178.