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2010 | 19 | 3 |

Tytuł artykułu

Intestinal and total tract phytate digestibility and phytase activity in the digestive tract of hens fed a wheat-maize-soyabean diet

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The intestinal and total tract digestibilities of phosphorus (P) and phytate P were determined in digestibility trials with laying hens and broiler breeders fed a diet containing P, phytate P and Ca at 6.37, 2.00 and 34.1 g·kg-1, respectively. In both digestibility trials, the total tract digestibility of phytate P was higher than the intestinal digestibility (33 and 35% vs 20 and 18%). In contrast, in both trials the total tract retention of P was lower than the intestinal retention (22 and 19% vs 52 and 42%). Phytate P represented 29.2% of the excreta P of laying hens and 23.6% of the excreta P of broiler breeders. The corresponding proportions of phosphate P were 48.7 and 46.6%, respectively. Samples of digesta and small intestinal mucosa of laying hens were diluted with physiological saline containing sodium phytate and incubated in vitro to determine the phytase activity. The average specific phytase activities in the crop, stomach, small intestinal contents, mucosa and caecal contents were 10.2, 9.2, 14.6, 11.5 and 135 μmol·h-1·g-1, respectively. The total phytase activities (per segment) in the small intestine (including the mucosa) and the caeca were 586 and 663 μmol·h-1, respectively. It can be concluded that phytase activity was present in all sections of the digestive tract. Consequently, in hens fed a wheat-maize-soyabean diet without a phytase supplement, phytate P was partially digestible. A part of the phytate degradation occurred in the hindgut.

Wydawca

-

Rocznik

Tom

19

Numer

3

Opis fizyczny

p.430-439,ref.

Twórcy

autor
  • Institute of Animal Physiology and Genetics, Academy of Sciences of The Czech Republic, Prague 4, CZ 142 20, Czech Republic
autor
autor
autor

Bibliografia

  • AOAC, 2005. Association of Official Analytical Chemists, Official Methods of Analysis. 18th Edition. Gaithersburg, MD
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  • Hurwitz S., Bar A., 1965. Absorption of calcium and phosphorus along the gastrointestinal tract of the laying fowl as influenced by dietary calcium and egg shell formation. J. Nutr. 86, 433-438
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  • Leytem A.B., Willing B.P., Thacker P.A., 2008b. Phytate utilization and phosphorus excretion by broiler chickens fed diets containing cereal grains varying in phytate and phytase content. Anim. Feed Sci. Tech. 146, 160-168
  • Marounek M., Skřivan M., Dlouhá G., Břeňová N., 2008a. Availability of phytate phosphorus and endogenous phytase activity in the digestive tract of laying hens 20 and 47 weeks old. Anim. Feed Sci. Tech. 146, 353-359
  • Marounek M., Skřivan M., Dlouhá G., Březina P., 2008b. Digestibility of phosphorus in laying hens fed a wheat-maize-soyabean diet and the excreta phosphorus fractions. J. Anim. Feed Sci. 17, 579-587
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  • Peterson G.L., 1978. A simplified method for analysis of inorganic phosphate in the presence of interfering substances. Anal. Biochem. 84, 164-172
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Bibliografia

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