The application of MALDI-TOF MS for dermatophyte identification

• Autorzy: Dabrowska I. , Dworecka-Kaszak B.
• Języki publikacji: EN

Abstrakty

EN

Dermatophytes are keratinolytic fungi responsible for a wide variety of diseases of the skin, nails and hair of mammals. Their identification is often complicated, labor-intensive and time consuming due to the high degree of intra-species morphological similarity, and also requires scientific knowledge and practice. The aim of this study was to demonstrate that MALDI-TOF MS technique may be a faster and more sophisticated method useful for the identification of dermatophytes and mycoses in general.

Słowa kluczowe

EN

application; MALDI-TOF MS zob.matrix-assisted laser desorption-ionization time-of-flight mass spectrometry; matrix-assisted laser desorption-ionization time-of-flight mass spectrometry; dermatophyte; identification; detection; fungi; mammal; skin disease; hair; nail

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2014
• Tom: 60
• Numer: 3
• Opis fizyczny: p.147-150,ref.

Twórcy

autor

Dabrowska I.

• Division of Microbiology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland

autor

Dworecka-Kaszak B.

• Division of Microbiology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland

Bibliografia

• [1] Garg J., Tilak R., Garg A., Prakash P., Gulati A.K., Nath G. 2009. Rapid detection of dermatophytes from skin and hair. BMC Research Notes 2: 60.
• [2] Havlickova B., Czaika V.A., Friedrich M. 2008. Epidemiological trends in skin mycoses worldwide. Mycoses 51: 2-15.
• [3] Dworecka-Kaszak B. 2008. Mikologia weterynaryjna. Wydawnictwo SGGW, Warszawa.
• [4] Mohanty J.C., Mohanty S.K., Sahoo R.C., Sahoo A., Prahara C.N. 1999. Diagnosis of superficial mycoses by direct microscopy – a statistical evaluation. Indian Journal of Dermatology Venereology and Leprology 65: 72-74.
• [5] Weitzman I., Summerbell R.C. 1995. The dermatophytes. Clinical Microbiology Reviews 8: 240-259.
• [6] Bistis G.N. 1959. Pleomorphisms in the dermatophytes. Mycologia 51: 440-444.
• [7] Cafarchia C., Iatta R., Latrofa M.S., Gräser Y., Otranto D. 2013. Molecular epidemiology, phylogeny and evolution of dermatophytes. Infection, Genetics and Evolution 20: 336-351.
• [8] Hryncewicz-Gwóźdź A., Jagielski T., Dobrowolska A., Szepietowski J.C., Baran E. 2011. Identification and differentiation of Trichophyton rubrum clinical isolates using PCR-RFLP and RAPD methods. European Journal of Clinical Microbiology and Infectious Diseases 30: 727-731.
• [9] Arabatzis M., Xylouri E., Frangiadaki I., Tzimogianni A., Milioni A., Arsenis G., Velegraki A. 2006. Rapid detection of Arthroderma vanbreuseghemii in rabbit skin specimens by PCR-RFLP. Veterinary Dermatology 17: 322-326.
• [10] Wisselink G.J., van Zanten E., Kooistra-Smid A.M.D. 2011. Trapped in keratin; a comparison of dermatophyte detection in nail, skin and hair samples directly from clinical samples using culture and realtime PCR. Journal of Microbiological Methods 85: 62-66.
• [11] Kanbe T., Suzuki Y., Kamiya A., Mochizuki T., Fujihiro M., Kikuchi A. 2003. PCR-based identification of common dermatophyte species using primer sets specific for the DNA topoisomerase II genes. Journal of Dermatological Science 32: 151-161.
• [12] Verrier J., Krähenbühl L., Bontems O., Fratti M., Salamin K., Monod M. 2013. Dermatophyte identification in skin and hair samples using a simple and reliable nested polymerase chain reaction assay. British Journal of Dermatology 168: 295-301.
• [13] De Respinis S., Tonolla M., Pranghofer S., Petrini L., Petrini O., Bosshard P.P. 2013. Identification of dermatophytes by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Medical Mycology 51: 514-521.
• [14] Alanio A., Beretti J.L., Dauphin B., Mellado E., Quesne G., Lacroix C., Amara A., Berche P., Nassif X., Bougnoux M.E. 2011. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for fast and accurate identification of clinically relevant Aspergillus species. Clinical Microbiology and Infection 17: 750-755.
• [15] Bader O., Weig M., Taverne-Ghadwal L., Lugert R., Gross U., Kuhns M. 2011. Improved clinical laboratory identification of human pathogenic yeasts by matrix-assisted laser desorption ionization timeof-flight mass spectrometry. Clinical Microbiology and Infection 17: 1359-1365.
• [16] Benagli C., Rossi V., Dolina M., Tonolla M., Petrini O. 2011. Matrix-assisted laser desorption ionizationtime of flight mass spectrometry for the identification of clinically relevant bacteria. Public Library of Science ONE 6: e16424.
• [17] Erhard M., Hipler U.C., Burmester A., Brakhage A.A., Wöstemeyer J. 2008. Identification of dermatophyte species causing onychomycosis and tinea pedis by MALDI-TOF mass spectrometry. Experimental Dermatology 17: 356-361.
• [18] Theel E.S., Hall L., Mandrekar J., Wengenack N.L. 2011. Dermatophyte identification using matrixassisted laser desorption ionization-time of flight mass spectrometry. Journal of Clinical Microbiology 49: 4067-4071.
• [19] Nenoff P., Erhard M., Simon J.C., Muylowa G.K., Herrmann J., Rataj W., Gräser Y. 2013. MALDI-TOF mass spectrometry – a rapid method for the identification of dermatophyte species. Medical Mycology 51: 17-24.
• [20] Alshawa K., Beretti J.-L., Lacroix C., Feuilhade M., Dauphin B., Quesne G., Hassouni N., Nassif X., Bougnoux M.E. 2012. Successful identification of clinical dermatophyte and Neoscytalidium species by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Journal of Clinical Microbiology 50: 2277-2281.
• [21] Hollemeyer K., Jager S., Altmeyer W., Heinzle E. 2005. Proteolytic peptide patterns as indicators for fungal infections and nonfungal affections of human nails measured by matrix-assisted laser desorption /ionization time-of-flight mass spectro metry. Analytical Biochemistry 338: 326-331.
• [22] Wieser A., Schneider L., Jung J., Schubert S. 2012. MALDI-TOF MS in microbiological diagnosticsidentification of microorganisms and beyond (mini review). Applied Microbiology and Biotechnology 93: 965-974.