Detection of Schmallenberg virus RNA in bull semen in Poland

• Autorzy: Kesik-Maliszewska J. , Larska M.
• Języki publikacji: EN

Abstrakty

EN

The detection of Schmallenberg virus (SBV) in the breeding bull semen raised the question of the possibility of venereal transmission of SBV which could result in cost-intensive restrictions in the trade of bovine semen. In order to evaluate the presence of SBV RNA in bovine semen, 131 bull semen samples from four locations in Poland collected between 2013 and 2015 were analysed by RT-PCR for viral RNA. SBV RNA was detected in 5.3% of the samples. The study has revealed that application of an appropriate RNA extraction method is crucial to detect virus excretion via semen.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2016
• Tom: 19
• Numer: 3
• Opis fizyczny: p.655-657,fig.,ref.

Twórcy

autor

Kesik-Maliszewska J.

• Department of Virology, National Veterinary Research Institute, Al. Partyzantow 75, 24-100 Pulawy, Poland

autor

Larska M.

• Department of Virology, National Veterinary Research Institute, Al. Partyzantow 75, 24-100 Pulawy, Poland

Bibliografia

• Hoffmann B, Schulz C, Beer M (2013) First detection of Schmallenberg virus RNA in bovine semen, Germany, 2012. Vet Microbiol 167: 289-295.
• Larska M, Kęsik-Maliszewska J, Kuta A (2014) Spread of Schmallenberg virus infections in the ruminants in Poland between 2012 and 2013. B Vet I Pulawy 58: 169-176.
• Ponsart C, Pozzi N, Breard E, Catinot V, Viard G, Sailleau C, Viarouge C, Gouzil J, Beer M, Zientara S, Vitour D (2014) Evidence of excretion of Schmallenberg virus in bull semen. Vet Res 45: 37.
• Qiagen (2013) User-Developed Protocol For manual purification of SBV RNA from bull semen: https://www.qiagen.com/fo/resources/download.aspx?id=64f26086-2199-4a4a-b5ec-ee10a6367228&lang=en
• Schulz C, van der Poel WH, Ponsart C, Cay AB, Steinbach F, Zientara S, Beer M, Hoffmann B (2015) European interlaboratory comparison of Schmallenberg virus (SBV) real-time RT-PCR detection in experimental and field samples: The method of extraction is critical for SBV RNA detection in semen. J Vet Diagn Invest 27: 422-430.
• Schulz C, Wernike K, Beer M, Hoffmann B (2014) Infectious Schmallenberg virus from bovine semen, Germany. Emerg Infect Dis 20: 338-340.
• Van Der Poel WH, Parlevliet JM, Verstraten ER, Kooi EA, Hakze-Van Der Honing R, Stockhofe N (2014) Schmallenberg virus detection in bovine semen after experimental infection of bulls. Epidemiol Infect 142: 1495-1500.

Identyfikatory

DOI

10.1515/pjvs-2016-0083