Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2012 | 59 | 2 |
Tytuł artykułu

Prebiotics and bioactive natural substances induce changes of composition and metabolic activities of the colonic microflora in cancerous rats

Treść / Zawartość
Warianty tytułu
Języki publikacji
Prebiotics are defined as selectively fermented food ingredients that induce specific changes in the composition and/or activity in the gastrointestinal microbiota beneficial to the host well-being and health. The aim of the presented experiment was to investigate the effect of a prebiotic applied alone or in combination with Hyppocastani extractum siccum, and Lini oleum virginale in rats with dimethylhydrazine induced colon cancer. Wistar albino rats were fed high fat diet supplemented with the prebiotic alone or in combination with Horse chestnut and flaxseed oil. The activity of faecal glycolytic enzymes, lipid parameters, bile acids, short chain fatty acids and counts of coliforms and lactobacilli were determined. Treatment with the prebiotic alone and in combination with selected substances significantly decreased the activity of glycolytic bacterial enzyme β-glucuronidase (P<0.001) and increased activities of β-galactosidase and β-glucosidase. Bile acids concentration was significantly decreased (P<0.01) except for the combination of the prebiotic with Horse chestnut. The prebiotic alone decreased the lipid parameters (P<0.001) and enhanced production of short chain fatty acids. Application of prebiotic and bioactive natural substances significantly reduced number of coliforms (P<0.05). Prebiotic alone significantly increased the count of lactobacilli (P<0.05). These results show that prebiotics have a protective effect and may be the useful for colon cancer prevention and treatment.
Słowa kluczowe
Opis fizyczny
  • Institute of Experimental Medicine, Faculty of Medicine, P.J.Safarik University, Kosice, Slovak Republic
  • Alvaro A, Sola R, Rosales R, Ribalta J, Anguera A, Masana L, Vallve JC (2008) Gene expression analysis of a human enterocytes cell line reveals downregulation of cholesterol biosynthesis in response to short-chain fatty acids. IUBMB Life 60: 757-764. 
  • Beylot M (2005) Effects of inulin-type fructans on lipid metabolism in man and in animal models. Br J Nutr 93 (Suppl 1): S163-S168. 
  • Bommareddy A, Arasada BL, Mathees DP, Dwivedi C (2006) Chemopreventive effects of dietary flaxseed on colon tumor development. Nutrition and Cancer 54: 216-222. 
  • Cheng K, Raufman JP (2005) Bile acid-induced proliferation of human colon cancer cell line is mediated by transactivation of epidermal growth factor receptors. Biochem Pharmacol 70: 1035-1047. 
  • Geier MS, Butler RN, Howarth GS (2006) Probiotics, prebiotics and synbiotics: a role in chemoprevention for colorectal cancer? Cancer Biol Ther 5: 1265-1269. 
  • Gibson GR, Roberfroid MB (1995) Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. J Nutr 125: 1401-1412. 
  • Gibson GR, Probert HM, Loo VJ, Rastall RA, Roberfroid MB (2004) Dietary modulation of the human colonic microbiota: updating the concept of prebiotics. Nutr Res Rev 17: 259-275. 
  • Hagiwara T (2006) Bile acids and colorectal cancer. Jpn J Cancer Clinics 51: 919-925.
  • Hijova E, Chmelarova A (2007) Short chain fatty acids and colonic health. Bratisl Med J 108: 354-358. 
  • Hu JN, Zhu XM, Han LK, Saito M, Sun YS, Yoshikawa M, Kimura Y, Zheng YN (2008) Anti-obesity effects of escins extracted from the seeds of Aesculus turbunata BLUME (Hippocastanaceae). Chem Pharm Bull (Tokyo) 56: 12-16. 
  • Lay C, Sutren M, Lepercq P, Juste C, Rigottier-Gois L, Lhoste E, Lemee R, Le Ruyet P, Dore J, Andrieux C (2004) Influence of Camembert consumption on the composition and metabolism of intestinal microbiota. A study in human microbiota-associated rats. Br J Nutr 92: 429-438. 
  • Loo VJ, Clune Y, Bennett M, Collins JK (2005) The SYNCAN project: goals, set-up, first results and setting of the human intervention study. Br J Nutr 93: (Suppl 1): S91-S98. 
  • Manju V, Nalini N (2006) Effect of ginger on bacterial enzymes in 1,2-dimethylhydrazine induced experimental colon carcinogenesis. Eur J Cancer Prev 15: 377-383. 
  • Nalini N, Manju V, Menon VP (2004) Effect of coconut cake on the bacterial enzymes activity in 1,2-dimethylhydrazine induced colon cancer. Clin Chim Acta 342: 203-210. 
  • Niu YP, Li LD, Wu LM (2008) Beta-aescin: A potent natural inhibitor of proliferation and inducer of apoptosis in human chronic myeloid leukemia K562 cells in vitro. Leuk Lymphoma 49: 1384-1391. 
  • Patlolla JM, Raju J, Swamy MV, Rao CV (2006) Beta-escin inhibits colonic aberrant crypt foci formation in rats and regulates the cell cycle growth by inducing p21 (waf1/cip1) in colon cancer cell. Mol Cancer Ther 5: 1459-1466. 
  • Pool-Zobel BL, Loo JV, Rowland IR, Roberfroid MB (2002) Experimental evidences on the potential of prebiotic fructans to reduce the risk of colon cancer. Br J Nutr 87: S273-S281. 
  • Pool-Zobel BL, Sauer J (2007) Overview of experimental data on reduction of colorectal cancer risk by inulin-type fructans. J Nutr 137: 2580S-2584S. 
  • Rafter J, Bennett M, Caderni G, Clune Y, Hughes R, Karlsson PC, Klinder A, O'Riordan M, Sullivan GC, Pool-Zobel B, Rechkemmer G, Roller M, Rowland I, Salvadori M, Thijs H, Loo VJ, Watzl B, Collins JK (2007) Dietary synbiotics reduce cancer risk factors in polypectomized and colon cancer patients. Am J Clin Nutr 85: 488-496. 
  • Rault-Nania MH, Gueux E, Demougeot C, Demigne Ch, Rock E, Mazur A (2006) Inulin attenuates atherosclerosis in apolipoprotein E-deficient mice. Br J Nutr 96: 840-844. 
  • Salminen S, Bouley C, Boutron-Ruault MC, Cummings JH, Franck A, Gibson GR, Isolauri E, Moreau MC, Roberfroid MB, Rowland IR (1998) Functional food science and gastrointestinal physiology and function. Br J Nutr 80: S147-S171. 
  • Theodoratou E, McNeill G, Cetnarskyj R, Farrington SM, Tenesa A, Barnetson R, Porteous M, Dunlop M, Campbell H (2007) Dietary fatty acids and colorectal cancer: a case-control study. Am J Epidemiol 166: 181-195. 
  • Trafalska E, Grzybowski A (2006) Probiotics and prebiotics in prevention of chronic civilization diseases. New Medicine 9: 3-6.
  • World Health Statistics (2008) WHO Press, Geneva, Switzerland, pp 110.
Rekord w opracowaniu.
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.