Cloning, expression in yeast, and functional characterization of CYP71D14, a root-specific cytochrome P450 from Petunia hybrida

• Autorzy: Imaishi H. , Matushita T.
• Języki publikacji: EN

Abstrakty

EN

Alkaloids, which are naturally occurring amines, are biosynthesized and accumulated in plant tissues such as roots, leaves, and stems. Many alkaloids have pharmacological effects on humans and animals. Cytochrome P450 (P450 or CYP) monooxygenases are known to play key roles in the biosynthesis of alkaloids in higher plants. A cDNA clone encoding a P450 protein consisting of 502 amino acids was isolated from Petunia hybrida. The deduced amino acid sequence of the cDNA clone showed a high level of similarity with the other P450 species in the CYP71D family; hence, this novel P450 was named CYP71D14. Among plant P450 species, CYP71D14 had 45.7% similarity in its amino acid sequence to CYP71D12, which is involved in the biosynthesis of the indole alkaloids vinblastine and vincristine. Expression of CYP71D14 mRNA in Petunia plants was examined by Northern blot analysis by using a full-length cDNA of CYP71D14 as aprobe. CYP71D14 mRNA was expressed most abundantly in the roots.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2008
• Tom: 30
• Numer: 3
• Opis fizyczny: p.333-338,fig.,ref.

Twórcy

autor

Imaishi H.

• Research Center for Environmental Genomics, Kobe University, Rokkodaicho 1-1, Nada-ku, 657-8501 Kobe, India

autor

Matushita T.

• Research Center for Environmental Genomics, Kobe University, Rokkodaicho 1-1, Nada-ku, 657-8501 Kobe, India

Bibliografia

• Bell-Lelong DA, Cusumano JC, Meyer K, Chapple C (1997) Cinnamate-4-hydroxylase expression in Arabidopsis. Regulation in response to development and the environment. Plant Physiol 113:729–738
• Bennett RN, Hick AJ, Dawson GW, Wallsgrove RM (1995) Glucosinolate biosynthesis (further characterization of the aldoxime-forming microsomal monooxygenases in oilseed rape leaves). Plant Physiol 109:299–305
• Flanagan JU, McLaughlin LA, Paine MJ, Sutcliffe MJ, Roberts GC, Wolf CR (2003) Role of conserved Asp293 of cytochrome P450 2C9 in substrate recognition and catalytic activity. Biochem J 370:921–926
• Hashimoto T, Yamada Y (1986) Hyoscyamine 6beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, in alkaloid-producing root cultures. Plant Physiol 81:619–625
• Holton TA, Lester DR (1996) Cloning of novel cytochrome P450 gene sequences via polymerase chain reaction amplification. Methods Enzymol 272:275–283
• Kahn RA, Bak S, Olsen CE, Svendsen I, Moller BL (1996) Isolation and reconstitution of the heme-thiolate protein obtusifoliol 14alpha-demethylase from Sorghum bicolor (L.) Moench. J Biol Chem 271:32944–32950
• Leon J, Shulaev V, Yalpani N, Lawton MA, Raskin I (1995) Benzoic acid 2-hydroxylase, a soluble oxygenase from tobacco, catalyzes salicylic acid biosynthesis. Proc Natl Acad Sci USA 92:10413–10417
• Morant M, Bak S, Moller BL, Werck-Reichhart D (2003) Plant cytochromes P450: tools for pharmacology, plant protection and phytoremediation. Curr Opin Biotechnol 14:151–162
• Poulos TL (1995) Cytochrome P450. Curr Opin Struct Biol 5:767–774
• Schroder G, Unterbusch E, Kaltenbach M, Schmidt J, Strack D, De Luca V, Schroder J (1999) Light-induced cytochrome P450- dependent enzyme in indole alkaloid biosynthesis: tabersonine 16-hydroxylase. FEBS Lett 458:97–102
• Schuler MA (1996) Plant cytochrome P450 monooxygenases. Crit Rev Plant Sci 15:235–284
• Song WC, Funk CD, Brash AR (1993) Molecular cloning of an allene oxide synthase: a cytochrome P450 specialized for the metabolism of fatty acid hydroperoxides. Proc Natl Acad Sci USA 90:8519–8523
• Suzuki K, Yun DJ, Chen XY, Yamada Y, Hashimoto T (1999) An Atropa belladonna hyoscyamine 6beta-hydroxylase gene is differentially expressed in the root pericycle and anthers. Plant Mol Biol 40:141–152
• Szabo L, Bolcskei H, Eszter BG, Mak M, Szantay C (2001) Synthesis of vinca alkaloids and related compounds, Part XCVI. Nitration study of vinblastine-type bisindole alkaloids. Arch Pharm (Weinheim) 334:399–405
• Szekeres M, Nemeth K, Koncz-Kalman Z, Mathur J, Kauschmann A, Altmann T, Redei GP, Nagy F, Schell J, Koncz C (1996) Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis. Cell 85:171–182
• Winkler RG, Helentjaris T (1995) The maize Dwarf3 gene encodes a cytochrome P450-mediated early step in Gibberellin biosynthesis. Plant Cell 7:1307–1317

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