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2013 | 35 | 11 |

Tytuł artykułu

Identification of candidate genes associated with male sterility in CMS7311 of heading Chinese cabbage (Brassica campestris L. ssp. pekinensis)

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Polima (pol) cytoplasmic male sterility (CMS) is reported to be regulated by orf224, but the molecular mechanisms of CMS are less known. In previous research, CMS7311 of heading Chinese cabbage (Brassica campestris L.ssp. pekinensis) was successfully generated from a pol-like CMS, named 77A. To further understand the molecular mechanisms of this male sterility, cDNA-AFLP and qRT-PCR were used to identify genes differentially expressed during flower bud development between cytoplasmic male-sterile plant (Ms) and male-fertile plant (Mf) derived from the hybrid of CMS7311 and a restorer (Rf). Thirty-two transcripts of different fragments (TDFs) of over 80 bp in length were identified using cDNA-AFLP. Among them, ten TDFs were found expressing in Ms bud, and the others expressing in Mf bud. Gene ontology analysis revealed that these genes were involved in stress response, synthesis and metabolism of fatty acid, cell reconstruction, etc. Further, five genes closely related to male sterile were selected and their expression during the flower bud development of Ms and Mf line were investigated by qRT-PCR. Three genes, BcGRP17, BcMS2, and BcPME31, which were necessary for normal male organ development, showed significantly lower expressions in Ms bud; two genes, BcROPGEF8 and BcTNL3, were induced with significantly higher expression in Ms bud by unknown stress and involved in the formation of male sterility. Further work will be focused on cloning these genes and identifying their functions in the male-sterile process of heading Chinese cabbage CMS7311.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

35

Numer

11

Opis fizyczny

p.3265-3270,fig.,ref.

Twórcy

autor
  • State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, Shaanxi, People’s Republic of China
  • College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, People’s Republic of China
autor
  • State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, Shaanxi, People’s Republic of China
  • College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, People’s Republic of China
autor
  • State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, Shaanxi, People’s Republic of China
  • College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, People’s Republic of China
autor
  • State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, Shaanxi, People’s Republic of China
autor
  • State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, Shaanxi, People’s Republic of China
  • College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, People’s Republic of China
autor
  • College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, People’s Republic of China

Bibliografia

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  • Cheng F, Liu S, Wu J, Fang L, Sun S, Liu B, Li P, Hua W, Wang X (2011) BRAD, the genetics and genomics database for Brassica plants. BMC Plant Biol 11:136. doi:10.1186/1471-2229-11-136
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  • Fujii S, Komatsu S, Toriyama K (2007) Retrograde regulation of nuclear gene expression in CW-CMS of rice. Plant Mol Biol 63(3):405–417. doi:10.1007/s11103-006-9097-8
  • Geddy R, Mahe L, Brown GG (2005) Cell-specific regulation of a Brassica napus CMS-associated gene by a nuclear restorer with related effects on a floral homeotic gene promoter. Plant J 41(3):333–345. doi:10.1111/j.1365-313X.2004.02305.x
  • Jiang M, Cao JS (2008) Isolation and characterization of a male sterility gene homolog BcMS2 from Chinese cabbagge-pak-choi that expressing in an anther-specific manner. Mol Biol Rep 35(3):299–305. doi:10.1007/s11033-007-9086-2
  • Liu LC, Yu XL, Ye WZ, Xiang X, Cao JS (2006) Functional confirmation of BcMF3 gene related to male sterility of Chinese cabbage by antisense RNA. J Zhejiang Univ (Agric Life Sci) 32(05):473–478 (in Chinese)
  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25(4):402–408. doi:10.1006/meth.2001.1262
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  • Singh M, Brown GG (1991) Suppression of cytoplasmic male-sterility by nuclear genes alters expression of a novel mitochondrial gene region. Plant Cell 3(12):1349–1362
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  • Tian GW, Chen MH, Zaltsman A, Citovsky V (2006) Pollen-specific pectin methylesterase involved in pollen tube growth. Dev Biol 294(1):83–91. doi:10.1016/j.ydbio.2006.02.026
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  • Wang YQ, Yu XL, Cao JS (2004) Isolation and characterization of BcMF3, a gene expressed only in maintainer line in Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino var. communis Tsen et lee). Yi Chuan Xue Bao 31(11):1302–1308 (in Chinese)
  • Zhang LG, Hao DF (2001) Investigation on the sterility changeover of male sterility line CMS7311 in heading Chinese cabbage. Acta Bot Sinica 43(11):1123–1128
  • Zhang ZF, Zhang LG, Wang Q, Hui MX, Zhang MK (2006) RNA fingerprinting of the differential expression fragments related to cytoplasmic male sterility in Chinese cabbage. Yi Chuan 28(10):1280–1286. doi:0253-9772(2006)10-1280-07(InChinese
  • Zhang CW, Qi L, Hou XL, Shi GJ, Zhang JY (2010) Differential gene expression analysis of a new Ogura CMS line and its maintainer in non-heading Chinese cabbage by cDNA-AFLP. Acta Physiologiae Plantarum 32(4):781–787. doi:10.1007/s11738-010-0463-4

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Typ dokumentu

Bibliografia

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