Intraspecific variability of cadmium tolerance in hydroponically grown Indian mustard (Brassica juncea (L.) Czern.) seedlings

• Autorzy: Belimov A.A. , Safronova V. I. , Demchinskaya S. V. , Dzyuba O. O.
• Języki publikacji: EN

Abstrakty

EN

Indian mustard (Brassica juncea (L.) Czern.) is a promising plant species for phytoremediation of heavy metal polluted soils. However, genetic variability of metal tolerance in Indian mustard has not been studied. We evaluated intraspecific variation of Cd tolerance of this species by screening 64 varieties in hydroponics. The tolerance index (TI), calculated as percentage of root length of Cd-treated (7 µM CdCl₂) over untreated control seedlings, significantly varied from 34 to 79%, depending on the variety. Information about phenotypic and economic traits of the studied varieties was taken from the literature and subjected to a cluster analysis. The varieties were distributed into three clusters and most of the varieties characterized by the highest TI values (TI > 65%) were grouped together in one cluster. Moreover, TI negatively correlated with the following characteristics: yellow seed colour (R = –0.35, P = 0.005), total oil content (R = –0.33, P = 0.008), oleic acid (R = –0.25, P = 0.047) and linoleic acid (R = –0.36, P = 0.004) contents in seeds. The results showed the presence of significant variability for Cd tolerance in Indian mustard. The knowledge about correlations between Cd tolerance and phenotypic characteristics of plants might be utilized for rapid selection of cultivars to be used for phytoremediation of polluted soils.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2007
• Tom: 29
• Numer: 5
• Opis fizyczny: p.473-478,fig.,ref.

Twórcy

autor

Belimov A.A.

• All-Russia Research Institute for Agricultural Microbiology, Podbelskogo Sh. 3, Pushkin-8, 196608 St-Petersburg, Russia

autor

Safronova V. I.

• All-Russia Research Institute for Agricultural Microbiology, Podbelskogo Sh. 3, Pushkin-8, 196608 St-Petersburg, Russia

autor

Demchinskaya S. V.

• All-Russia Research Institute for Agricultural Microbiology, Podbelskogo Sh. 3, Pushkin-8, 196608 St-Petersburg, Russia

autor

Dzyuba O. O.

• N.I. Vavilov Research Institute of Plant Industry, B. Morskaya Str. 42, 190000 St-Petersburg, Russia

Bibliografia

• Antonovics J, Bradshaw AD (1970) Evolution of closely adjacent plant populations. VIII. Cline patterns in Anthoxanthum odoratum across a mine boundary. Heredity 25:349–358
• Belimov AA, Safronova VI, Tsyganov VE, Borisov AY, Kozhemyakov AP, Stepanok VV, Martenson AM, Gianinazzi-Pearson V, Tikhonovich IA (2003) Genetic variability in tolerance to cadmium and accumulation of heavy metals in pea (Pisum sativum L.). Euphytica 131:25–35
• Bennett LE, Burkhead JL, Hale KL, Terry N, Pilon M, Pilon-Smits E (2003) Analysis of transgenic Indian mustard plants for phytoremediation of metal-contaminated mine tailings. J Environ Qual 32:432–440
• Bolan NS, Adriano DC, Duraisamy P, Mani A, Arulmozhiselvan K (2003) Immobilization and phytoavailability of cadmium in variable charge soils. I. Effect of phosphate addition. Plant Soil 250:83–94
• Cardoso PF, Molina SMG, Pereira GJG, Vitoria AP, Azevedo RA (2002) Response of rice inbred lines to cadmium exposure. J Plant Nutr 25:927–944
• Cobbett CS (2000) Phytochelatins and their roles in heavy metal detoxification. Plant Physiol 123:825–832
• Daun JK, DeClercq DR (1988) Quality of yellow and dark seeds in Brassica campestris canola varieties Candle and Tobin. J Am Oil Chem Soc 65:122–126
• De Knecht JA, van Baren N, Bookum WMT, Sang HWWF, Koevoets PLM, Schat H, Verkleij JAC (1995) Synthesis and degradation of phytochelatins in cadmium-sensitive and cadmium-tolerant Silene vulgaris. Plant Sci 106:9–18
• Ebbs SD, Lasat MM, Brady DJ, Cornish J, Gordon RK, Kochian LV (1997) Phytoextraction of cadmium and zinc from a contaminated soil. J Environ Qual 26:1424–1430
• Epstein AL, Gussman CD, Blaylock MJ, Yermiyahu U, Huang JW, Kapulnik Y, Orser CS (1999) EDTA and Pb-EDTA accumulation in Brassica juncea grown in Pb-amended soil. Plant Soil 208:87–94
• Fusco N, Micheletto L, Corso GD, Borgato L, Furini A (2005) Identification of cadmium-regulated genes by cDNA-AFLP in the heavy metal accumulator Brassica juncea L. J Exp Bot 56:3017–3027
• Gepts P (2002) A comparison between crop domestication, classical plant breeding, and genetic engineering. Crop Sci 42:1780–1790
• Hall JL (2002) Cellular mechanisms for heavy metal detoxification and tolerance. J Exp Bot 53:1–11
• Heiss S, Schafer HJ, Haag-Kerwer A, Rausch T (1999) Cloning sulphur assimilation genes of Brassica juncea L.: cadmium differentially affects the expression of a putative low-affinity sulphate transporter and isoforms of ATP sulfurylase and APS reductase. Plant Mol Biol 39:847–857
• Hsu YT, Kao CH (2003) Changes in protein and amino acid contents in two cultivars of rice seedlings with different apparent tolerance to cadmium. Plant Growth Reg 40:147–155
• Huff DR, Wu L (1985) Phenotypic correlation between metal tolerance and morphology in Festuca rubra L. Crop Sci 25:787–795
• Kartamysheva EV, Shurupov VG, Gorbachenko VD, Demidova EN, Cherkasov AI, Krasnov VP Gorchitsa sareptskaya (saperda mustard). Catalogue of the VIR world collection. vol 683 (VIR Press, St-Petersburg), 1996
• Kaur S, Nicolas ME, Ford R, Norton RM, Taylor PWJ (2006) Selection of Brassica rapa genotypes for tolerance to boron toxicity. Plant Soil 285:115–123
• Kidd PS, Diez J, Martinez CM (2004) Tolerance and bioaccumulation of heavy metals in five populations of Cistus ladanifer L. subsp. ladanifer. Plant Soil 258:189–205
• Kumar PBA, Dushenkov V, Motto H, Raskin I (1995) Phytoextraction: the use of plants to remove heavy metals from soils. Environ Sci Technol 29:1232–1238
• Lee S, Leustek T (1999) The affect of cadmium on sulphate assimilation enzymes in Brassica juncea. Plant Sci 141:201–207
• Lindberg S, Greger M (2002) Plant genotypic differences under metal deficient and enriched conditions. In: Prasad MNV, Stralska K (eds) Physiology and biochemistry of vetal toxicity and tolerance in plants. Kluwer, Dordrecht, pp 357–393
• Mahmood T, Ekuere U, Yeh F, Good AG, Stringam GR (2003) RFLP linkage analysis and mapping genes controlling the fatty acid profile of Brassica juncea using reciprocal DH populations. Theor Appl Genet 107:283–290
• Mandal S, Yadav S, Singh R, Begum G, Suneja P, Singh M (2002) Correlation studies on oil content and fatty acid profile of some Cruciferous species. Genet Res Crop Evol 49:551–556
• Marchiol L, Assolari S, Fellet G, Zerbi G (2006) Germination and seedling growth of Indian mustard exposed to cadmium and chromium. Ital J Agron 1:45–49
• Metwally A, Safronova VI, Belimov AA, Dietz K-J (2005) Genotypic variation of the response to cadmium toxicity in Pisum sativum L. J Exp Bot 56:167–178
• Moroni JS, Scott BJ, Watten N (2003) Differential tolerance of high manganese among rapeseed genotypes. Plant Soil 253:507–519
• Panwar BS, Ahmed KS, Mittal SB (2002) Phytoremediation of nickel-contaminated soils by Brassica species. Environ Dev Sustain 4:1–6
• Panwar BS, Ahmed KS, Sihag D, Patel AL (2005) Distribution of cadmium and nickel among various forms in natural and contaminated soils amended with EDTA. Environ Dev Sustain 7:153–160
• Podar D, Ramsey MH, Hutchings MJ (2004) Effect of cadmium, zinc and substrate heterogeneity on yield, shoot metal concentration and metal uptake by Brassica juncea: implications for human health risk assessment and phytoremediation. New Phytol 163:313–324
• Provorov NA, Tikhonovich IA (2003) Genetic resources for improving nitrogen fixation in legume–rhizobia symbiosis. Gen Res Crop Evol 50:89–99
• Sabharwal V, Negi MS, Banga SS, Lakshmikumaran M (2004) Mapping of AFLP markers linked to seed coat colour loci in Brassica juncea (L.) Czern. Theor Appl Genet 109:160–166
• Salt DE, Prince EC, Pickering IJ, Raskin I (1995) Mechanisms of cadmium mobility and accumulation in Indian mustard. Plant Physiol 109:1427–1433
• Sanita di Toppi L, Gabbrielli R (1999) Response to cadmium in higher plants. Environ Exp Bot 41:105–130
• Wu L (1990) Colonization and establishment of plants in contaminated sites. In: Shaw AJ (ed) Heavy metal tolerance in plants: evolutionary aspects. CRC, Boca Raton, pp 269–284
• Zhu YL, Pilon-Smith EAH, Tarun AS, Weber SU, Jouanin L, Terry N (1999) Cadmium tolerance and accumulation in Indian mustard is enhanced by overexpressing γ-glutamylcysteine synthetase. Plant Physiol 121:1169–1177