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2010 | 13 | 4 |

Tytuł artykułu

Occurrence of leptospiral infections in swine population in Poland evaluated by ELISA and microscopic agglutination test

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Swine are one of significant reservoirs and sources of Leptospira infections for man. Serological screenings help to effectively control the epidemiological situation in swine herds and to prevent transmission of Leptospira from animals to man. The purpose of this study was to investigate, by the use of serological methods, the prevalence of infections caused by selected Leptospira serogroups in swine population in Poland. A total of 7112 swine serum samples were examined. The samples were collected from January to October 2008 and came from 280 counties situated in all 16 provinces of Poland. All sera were examined preliminary by enzyme-linked immunosorbent assay (ELISA) using heat-stable antigenic preparation. The samples positive or doubtful in ELISA were investigated by microscopic agglutination test (MAT) with use of serovars Icterohaemorrhagiae, Pomona, Canicola, Sejroe, Tarassovi and Grippotyphosa. Of the collected sera examined by ELISA 73 (1.02%) samples were positive, 85 (1.20%) – doubtful and 6954 – negative. Among ELISA-positive and doubtful sera 64 samples (coming from 14 provinces) were recognized in MAT as positive. Among MAT positive samples 42.19% of sera demonstrated titres with serovar Pomona, 32.81% – with Sejroe, 14.06% – with Icterohaemorrhagiae, 6.25% – with Tarassovi, 3.13% – with Grippotyphosa and 1.56% with Canicola.

Wydawca

-

Rocznik

Tom

13

Numer

4

Opis fizyczny

p.695-699,ref.

Twórcy

autor
  • Department of Swine Diseases, National Veterinary Research Institute, Al.Partyzantow 57, 24-100 Pulawy, Poland
autor

Bibliografia

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  • Hartman EG, van Houten M, van der Donk JA, Frik JF (1984) Determination of specific anti-leptospiral immunoglobulins M and G in sera of experimentally infected dogs by solid-phase enzyme-linked immunosorbent assay. Vet Immunol Immunopathol 7: 43-51.
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  • Theodoridis D, Bohmer J, Homuth M, Strutzberg-Minder K (2005) Development of a novel ELISA for serodiagnosis of Leptospirosis and additional detection of pathogenic Leptospira by polymerase chain reaction for veterinary routine diagnostics. Rev Cubana Med Trop 57: 49-50.
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  • Wasinski B (2007) Occurrence of Leptospira sp. antibodies in swine in Poland. Bull Vet Inst Pulawy 51: 225-228.

Typ dokumentu

Bibliografia

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