Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2020 | 76 | 06 |
Tytuł artykułu

Molecular characterization of contagious ecthyma virus (CEV) isolated from goat and cross-protection studies

Warianty tytułu
Języki publikacji
Contagious ecthyma (CE) is a zoonotic viral infection caused by the Parapoxvirus in sheep and goats which is classified in the family of Poxviridae. In this study, the Penorf CE vaccine strain that originated from lambs (PKCE1 strain) and three CE strains (O-CEV1, O-CEV2, O-CEV3) originated from kids were used for molecular characterization and cross-protection studies. A phylogenetic similarity has been investigated by comparing the B2L gene of CE viruses originated from kids and lambs. It was observed that the isolates O-CEV1 and O-CEV2 had a similar DNA sequence (100%) whereas the other isolate, O-CEV3 had a different DNA sequence from the others, and the proportion of the difference between them was 2.6% as stated in the similarity index. The phylogenic evaluation revealed that CE viruses were not species specific and have different genotypes in lambs and kids in Turkey. Penorf vaccine strain which is still known as lamb origin was found to be also kid origin. In the pathogenity studies in kids and lambs, there was no rise in the body temperatures of lambs and kids and hyperemia, vesicles and pustules occurred in the scarified skin regions from the second day of the epruvation. In addition to these findings, it was determined that the healing in lesions occurred after the scabs fell off beginning from the 38th to 55th days of the study. At the end of the this study, the presence of CE strains with different pathogenicity properties was revealed. In goats vaccinated with Penorf vaccine, protection to O-CEV3 field isolate has been observed, but not obtained protection to PK-CK1 strain. As a result, the phylogenic evaluation revealed that CE viruses were not species specific and have different genotypes in lambs and kids in Turkey. The Penorf vaccine strain, which is still known to be of lamb origin, was found to be of kid origin and it was not seen to protect lambs against CE disease due to the genomic differences. Therefore, it was concluded from this data that at least a bivalent CE vaccine containing lamb and kid isolates should be prepared and used for effective immunity against CE infection, especially in lambs and kids.
Słowa kluczowe
Opis fizyczny
  • General Directorate of Food and Control, Ministry of Agriculture and Forestry, Cankaya, 06800 Ankara, Turkey
  • Department of Virology, Faculty of Veterinary Medicine, Istanbul University Cerrahpasa, Hadimkoy, 34320 Istanbul , Turkey
  • Pendik Veterinary Control Institute, Pendik, 34890 Istanbul , Turkey
  • Pendik Veterinary Control Institute, Pendik, 34890 Istanbul , Turkey
  • 1. Buddle B. M., Dellers R. W., Schuring G. G.: Heterogenicity of contagious ecthyma virus isolates. Am. J. Vet. Res. 1984, 45, 75-79.
  • 2. Buddle B. M., Pulford H. D.: Effect of passively acquired antibodies and vaccination on the immune response to contagious ecthyma virus. Vet. Microbiol. 1984, 9, 515-522.
  • 3. Burleson F. G., Chambers T. M., Wıedbrauk D. L.: Virologia Laboratory Manual. Academic Press. California 1992, 20-61.
  • 4. Cargnelutti J. F., Masuda E. K., Martins M., Diel D. G., Rock D. L., Weiblen R., Flores E. F.: Virological and clinico-pathological features of orf virus infection in experimentally infected rabbits and mice. Microb. Pathog. 2011, 50, 56-62.
  • 5. Chan K. W., Lin J. W., Lee S. H., Liao C. J., Tsai M. C., HSU W. L., Wong M. L., Shih H. C.: Identification and phylogenetic analysis of orf virus from goats in Taiwan. Virus Genes 2017, 35, 705-712.
  • 6. Coates J. W., Haff S.: Contagious ectyhma: an unusual distribution of lesions in goats. Can. Vet. J. 1990, 31, 209-210.
  • 7. De La Concha-Bermejillo A., Guo J., Zhang Z., Waldron D.: Severe persistent orf in young goats. J. Vet. Diagn. Invest. 2003, 15, 423-431.
  • 8. Demiraslan H., Dinc G., Doganay M.: An overview of orf virus infection in humans and animals. Recent Pat Antiinfection. Drug Discov. 2017, 12, 21-30.
  • 9. Ergin H., Koklu A.: Investigation of tissue culture passage and antigenic properties of ectima virus. Pendik Vet. Mikrobiol. 1974, 6, 12-20.
  • 10. Gameel A. A., Abu Elzein E. M. E., Housawi F. M. T., Agip A., Ibrahim A.: Clinico-pathological observations on naturally occurring contagious ecthyma in lambs in Saudi Arabia. Revue Elev. Med. Vet. Pay. Trop. 1995, 48, 233-235.
  • 11. Haig D. M., Melnnes C. J.: Immunity and counter-immunity during infection with the parapoxvirus orf virus. Virus Research 2002, 88, 3-16.
  • 12. Hosamani M., Bhanuprakash V., Scagliarini A., Sing R. K.: Comparative sequence analysis of major envelope protein gene (B2L) of Indian orf viruses isolated from sheep and goats. Vet. Microbiol. 2006, 116, 317-324.
  • 13. Housawi F. M. T., Abu Elzein E. M. E., Gameel A. A., Ala Faleg A. I.: A Close comparative study on the response of sheep and goats to experimental orf infection. J. Vet. Med. 1993, 40, 272-282.
  • 14. Hussain K. A., Burger D.: In vivo and invitro characteristics of contagious ecthyma virus isolates: host response mechanism. Vet. Microbiol. 1989, 19, 23-36.
  • 15. Issi M., Gulacti I., Bulut H., Gul Y.: Severe Persistent Contagious Ecthyma Cases in Twin Goats. J. Anim. and Vet. Adv. 2010, 9, 2477-2481.
  • 16. Mazur C., Ferreira I. I., Rangel F. B., Galler R.: Molecular characterization of Brazilian isolates of orf virus. Vet. Microbiol. 2000, 73, 253-259.
  • 17. Mckeever D. J., Jan Kinson D. M., Hutchison G., Reid H. W.: Studies of the pathogenesis of orf virus infection in sheep. J. Comp. Pathol. 1988, 99, 317-328.
  • 18. Mckeever D. J., Reid H. W., Ingus N. F., Herring A. J.: A qualitative and quantitative assessment of the humoral antibody response of the sheep to orf virus Infection. Vet. Microbiol. 1987, 15, 229-241.
  • 19. Mondal B., Bera A. K., Hosamani M., Tembhurne P. A., Bandyopadhyay S. K.: Detection of orf virus from an outbreak in goats and its genetic relation with other parapox viruses. Vet. Res. Commun. 2006, 30, 531-539.
  • 20. Musser J. M. B., Taylor C. A., Gua J., Tizard I. R., Walker J. W.: Development of a contagious ecthyma vaccine for goats. Am. J. Vet. Res. 2008, 69, 1366-1370.
  • 21. Musser J. M. B., Waldron D. F., Taylor C. A.: Evaluation of homologous and heterologous protection induced by a virulent field strain of orf virus and an orf vaccine in goats. Am J. Vet. Res. 2012, 73, 86-90.
  • 22. Nashirudddullah N., Pathak D. C., Barman N. N., Ahmet J. A., Raibongshi G., Sharna R. K., Borah P., Begum S. S.: Evaluation of orf virus (ORFV) isolation in continuous lamb testis cells (OA3.Ts) and development of a co-culture method with infected cells to increase infectivity. Indian J. Anim. Res. 2016, 50, 951-957.
  • 23. Nettleton P. F., Brebner J., Pow I., Gilray J. A., Bell G. D., Reid H. W.: Tissue culture-propagated orf virus vaccine protects lambs from orf virus challenge. Vet. Record. 1996, 138, 184-186.
  • 24. Peralta A., Robles C. A., Juan F., Micheluod J. F., Rossanigo C. E., MartinezA., Carosio A., König G. A.: Phylogenetic analysis of orf viruses from five contagious ecthyma outbreaks in Argentinian goats. Front. Vet. Sci. 2018, 5, 134.
  • 25. Pye D.: Vaccination of sheep with cell culture grown orf virus. Aust. Vet. J. 1990, 67, 182-183.
  • 26. Rabinson A. J., Balassu T. C.: Contagious pustular dermatitis (Orf). Vet. Bull. 1981, 51, 771-782.
  • 27. Rafii F., Burger D.: Comparison of contagious ecthyma virus genomes by restriction endonucleases. Arch. Viral. 1984, 84, 283-289.
  • 28. Seifert H. S. H.: Contagious ecthyma (CE) orf. Tropical Animal Health, Kluver Academic Publish, Dordrecht 1996, p. 402-403.
  • 29. Yırrell D. L., Reid H. W., Norval M., Idowie S. E. M.: Immun responses of lambs to experimental infection with orf virus. Vet. Immunol. Immunopathol. 1989, 22, 321-322.
  • 30. Zamri S. M., Rashidah I., AL-Ajeeli K. S. A.: Experimental cross-infection of sheep and goats with different isolates of contagious ecthyma virus. Aust. Vet. J. 1994, 71, 218-220.
  • 31. Zhao K., He W., Gao W., Lu H., Han T., Li J., Zhang X., Zhang B., Wang G., Su G., Zhao Z., Song D., Gao F.: Orf virus DNA vaccines expressing ORFV 011 and ORFV 059 chimeric protein enhances immunogenicity. Virol. J. 2011, 8, 562.
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.