Molecular cloning, characterization and expression analysis of a new gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from Salvia miltiorrhiza

• Autorzy: Liao P. , Zhou W. , Zhang L. , Wang J. , Yan X. , Zhang Y. , Zhang R. , Li L. , Zhou G. , Kai G.
• Języki publikacji: EN

Abstrakty

EN

The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) catalyzes the conversion of HMG-CoA to mevalonate (MVA), which is the first committed step in MVA pathway for isoprenoid biosynthesis in plants. In this study, a full-length cDNA encodingHMGRwas isolated from Salvia miltiorrhiza by rapid amplification of cDNA ends (RACE) for the first time, which was designated as SmHMGR (Gen-Bank Accession No.EU680958). The full-length cDNA of SmHMGR was 2,115 bp containing a 1,695 bp open reading frame (ORF) encoding a polypeptide of 565 amino acids. Bioinformatic analyzes revealed that the deduced SmHMGR had extensive homology with other plant HMGRs contained two transmembrane domains and a catalytic domain. Molecular modeling showed thatSmHMGRis a newHMGR with a spatial structure similar to other plant HMGRs. Phylogenetic tree analysis indicated that SmHMGR belongs to the plant HMGR super-family and has the closest relationship with HMGR from Picrorhiza kurrooa. Expression pattern analysis implied that SmHMGR expressed highest in root, followed by stem and leaf. The expression of SmHMGR could be up-regulated by salicylic acid (SA) and methyl jasmonate (MeJA), suggesting that SmHMGR was elicitorresponsive. This work will be helpful to understand more about the role of HMGR involved in the tanshinones biosynthesis at the molecular level.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 3
• Opis fizyczny: p.565-572,fig.,ref.

Twórcy

autor

Liao P.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, 200234 Shanghai, People's Republic of China

autor

Zhou W.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, 200234 Shanghai, People's Republic of China

autor

Zhang L.

• Department of Pharmacy, Shaoxing People's Hospital, 312000 Shaoxing, People's Republic of China

autor

Wang J.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, 200234 Shanghai, People's Republic of China

autor

Yan X.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, 200234 Shanghai, People's Republic of China

autor

Zhang Y.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, 200234 Shanghai, People's Republic of China

autor

Zhang R.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, 200234 Shanghai, People's Republic of China

autor

Li L.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, 200234 Shanghai, People's Republic of China

autor

Zhou G.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, 200234 Shanghai, People's Republic of China

autor

Kai G.

• Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, 200234 Shanghai, People's Republic of China

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