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2012 | 59 | 2 |
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Triterpenoid alpha-amyrin stimulates proliferation of human keratinocytes but does not protect them against UVB damage

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Rhaponticum carthamoides plants ("maral root") are widely used in Siberian folk medicine. The present study reports for the first time the presence of pentacyclic terpenoid, α-amyrin, in methanol extract from leaves of this plant. α-Amyrin induced proliferation of human keratinocytes (HaCaT) by about 18% while other extract components were ineffective. A panel of biochemical and cell-based assays testing the antioxidative and cytoprotective activites of α-amyrin indicated no antioxidative activity of this compound. α-Amyrin did not protect HaCaT cells against the damage caused by UVB radiation.
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  • Department of Dermatology, Bispebjerg Hospital, Copenhagen, Denmark
  • Agyare C, Lechtenberg M, Deters A, Petereit F, Hensel A (2011) Ellagitannins from Phyllanthus muellerianus (Kuntze) Exell.: Geraniin and furosin stimulate cellular activity, differentiation and collagen synthesis of human skin keratinocytes and dermal fibroblasts. Phytomedicine 18: 617-624. 
  • Akihisa T, Kojima N, Kikuchi T, Yasukawa K, Tokuda H, T Masters E, Manosroi A, Manosroi J (2010) Anti-inflammatory and chemopreventive effects of triterpene cinnamates and acetates from shea fat. J Oleo Sci 59: 273-80.  
  • Allouche Y, Warleta F, Campos M, Sánchez-Quesada C, Uceda M, Beltrán G, Gaforio JJ (2011) Antioxidant, antiproliferative, and pro-apoptotic capacities of pentacyclic triterpenes found in the skin of olives on MCF-7 human breast cancer cells and their effects on DNA damage. J Agric Food Chem 59: 121-130. 
  • Anaya AL, Mata R, Sims JJ, González-Coloma A, Cruz-Ortega R, Guadaño A, Hernández-Bautista BE, Midland SL, Ríos R, Gómez-Pompa A (2003) Allelochemical potential of Callicarpa acuminata. J Chem Ecol 29: 2761-2776. 
  • Aslam MN, Lansky EP, Varani J (2006) Pomegranate as a cosmeceutical source: pomegranate fractions promote proliferation and procollagen synthesis and inhibit matrix metalloproteinase-1 production in human skin cells. J Ethnopharmacol 103: 311-318. 
  • Babior BM (1984) The respiratory burst of phagocytes. J Clin Invest 73: 599-601. 
  • Barros FW, Bandeira PN, Lima DJ, Meira AS, de Farias SS, Albuquerque MR, Santos HS, Lemos TL, de Morais MO, Costa-Lotufo LV, Pessoa Cdo Ó (2011) Amyrin esters induce cell death by apoptosis in HL-60 leukemia cells. Bioorg Med Chem 19: 1268-1276. 
  • Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem 239: 70-76. 
  • Biskup E, Szynklarz B, Kumirska J, Golebiowski M, Stepnowski P and Lojkowska E (2007) Comparison of biological activity and composition of extracts from Rhaponticum carhamoides grown on Polish and Siberian plantations. Planta Med 73: 909.
  • Biskup E, Lojkowska E (2009) Evaluation of biological activities of Rhaponticum carthamoides extracts. J Med Plant Res 3: 1092-1098.
  • Boukamp P, Petrussevska RT, Breitkreutz D, Hornung J, Markham A, Fusenig NE (1988) Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol 106: 761-771. 
  • Brunold C, Deters A, Knoepfel-Sidler F, Hafner J, Müller B, Hensel A (2004) Polysaccharides from Hibiscus sabdariffa flowers stimulate proliferation and differentiation of human keratinocytes. Planta Med 70: 370-373.  
  • Cai BX, Luo D, Lin XF, Gao J (2008) Compound K suppresses ultraviolet radiation-induced apoptosis by inducing DNA repair in human keratinocytes. Arch Pharm Res 31: 1483-1488. 
  • Chaturvedula VS, Schilling JK, Miller JS, Andriantsiferana R, Rasamison VE, Kingston DG (2004) New cytotoxic terpenoids from the wood of Vepris punctata from the Madagascar Rainforest. J Nat Prod 67: 895-898. 
  • Chobot V, Buchta V, Jahodárová H, Pour M, Opletal L, Jahodár L, Harant P (2003) Antifungal activity of a thiophene polyine from Leuzea carthamoides. Fitoterapia 74: 288-290. 
  • Chung IM, Kim MY, Park SD, Park WH, Moon HI (2009) In vitro evaluation of the antiplasmodial activity of Dendropanax morbifera against chloroquine-sensitive strains of Plasmodium falciparum. Phytother Res 23: 1634-1637. 
  • Dinkova-Kostova AT, Jenkins SN, Wehage SL, Huso DL, Benedict AL, Stephenson KK, Fahey JW, Liu H, Liby KT, Honda T, Gribble GW, Sporn MB, Talalay P (2008) A dicyanotriterpenoid induces cytoprotective enzymes and reduces multiplicity of skin tumors in UV-irradiated mice. Biochem Biophys Res Commun 367: 859-865. 
  • Faurschou A, Gniadecki R, Calay D, Wulf HC (2008) TNF-alpha impairs the S-G2/M cell cycle checkpoint and cyclobutane pyrimidine dimer repair in premalignant skin cells: role of the PI3K-Akt pathway. J Invest Dermatol 128: 2069-2077. 
  • Holanda Pinto SA, Pinto LM, Guedes MA, Cunha GM, Chaves MH, Santos FA, Rao VS (2008) Antinoceptive effect of triterpenoid alpha,beta-amyrin in rats on orofacial pain induced by formalin and capsaicin. Phytomedicine 15: 630-634.  
  • Jodynis-Liebert J, Murias M, Błoszyk E (2000) Effect of sesquiterpene lactones on antioxidant enzymes and some drug-metabolizing enzymes in rat liver and kidney. Planta Med 66: 199-205. 
  • Kehrer JP (1993) Free radicals as mediators of tissue injury and disease. Crit Rev Toxicol 23: 21-48. 
  • Koleckar V, Brojerova E, Rehakova Z, Kubikova K, Cervenka F, Kuca K, Jun D, Hronek M, Opletalova V, Opletal L (2010) In vitro antiplatelet activity of flavonoids from Leuzea carthamoides. Drug Chem Toxicol 31: 27-35. 
  • Konczak-Islam I, Yoshimoto M, Hou DX, Terahara N, Yamakawa O (2003) Potential chemopreventive properties of anthocyanin-rich aqueous extracts from in vitro produced tissue of sweetpotato (Ipomoea batatas L.). J Agric Food Chem 51: 5916-5922. 
  • Lee J, Jung E, Lee J, Huh S, Kim J, Park M, So J, Ham Y, Jung K, Hyun CG, Kim YS, Park D (2007) Panax ginseng induces human Type I collagen synthesis through activation of Smad signaling. J Ethnopharmacol 109: 29-34. 
  • Leeuwenburgh C, Hansen P, Shaish A, Holloszy JO, Heinecke JW (1998) Markers of protein oxidation by hydroxyl radical and reactive nitrogen species in tissues of aging rats. Am J Physiol 274: R453-R461. 
  • Lu Y, Foo Y (2001) Antioxidant activities of polyphenols from sage (Salvia officinalis). Food Chem 75: 197-202.
  • Melo CM, Carvalho KM, Neves JC, Morais TC, Rao VS, Santos FA, Brito GA, Chaves MH (2010) Alpha,beta-amyrin, a natural triterpenoid ameliorates L-arginine-induced acute pancreatitis in rats. World J Gastroenterol 16: 4272-4280. 
  • Miliauskas G, van Beek TA, de Waard P, Venskutonis RP, Sudhölter EJ (2005) Identification of radical scavenging compounds in Rhaponticum carthamoides by means of LC-DAD-SPE-NMR. J Nat Prod 68: 168-172.  
  • Müller K, Gürster D (1993) Hydroxyl radical damage to DNA sugar and model membranes induced by anthralin (dithranol). Biochem Pharmacol 46: 1695-1704. 
  • Nikiéma JB, Vanhaelen-Fastré R, Vanhaelen M, Fontaine J, De Graef C, Heenen M (2001) Effects of antiinflammatory triterpenes isolated from Leptadenia hastata latex on keratinocyte proliferation. Phytother Res 15: 131-134.  
  • Oliveira FA, Chaves MH, Almeida FR, Lima RC Jr, Silva RM, Maia JL, Brito GA, Santos FA, Rao VS (2005) Protective effect of alpha- and beta-amyrin, a triterpene mixture from Protium heptaphyllum (Aubl.) March. trunk wood resin, against acetaminophen-induced liver injury in mice. J Ethnopharmacol 98: 103-108.  
  • Pavlík M, Laudová V, Grüner K, Vokác K, Harmatha J (2002) High-performance liquid chromatographic analysis and separation of N-feruloylserotonin isomers. J Chromatogr B Analyt Technol Biomed Life Sci 770: 291-295.  
  • Prieto P, Pineda M, Aguilar M (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 269: 337-341. 
  • Reuter J, Wölfle U, Weckesser S, Schempp C (2010) Which plant for which skin disease? Part 1: Atopic dermatitis, psoriasis, acne, condyloma and herpes simplex. J Dtsch Dermatol Ges 8: 788-796.  
  • Sharaf M, Skiba A, Weglarz Z, El-Ansari MA (2001) Two flavonol 5-O-glycosides from the roots of Leuzea carthamoides. Fitoterapia 72: 940-942. 
  • Thannickal VJ, Fanburg BL (2000) Reactive oxygen species in cell signaling. Am J Physiol Lung Cell Mol Physiol 279: L1005-L1028. 
  • Umemura K, Itoh T, Hamada N, Fujita Y, Akao Y, Nozawa Y, Matsuura N, Iinuma M, Ito M (2008) Preconditioning by sesquiterpene lactone enhances H2O2-induced Nrf2/ARE activation. Biochem Biophys Res Commun 368: 948-954. 
  • Villaseñor IM, Canlas AP, Faustino KM, Plana KG (2004) Evaluation of the bioactivity of triterpene mixture isolated from Carmona retusa (Vahl.) Masam leaves. J Ethnopharmacol 92: 53-56.  
  • Yance D (2004) Rhaponticum carthamoides: Monograph and Review of the Literature. American Herbalists Guide 5: 9-17.
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