Effect of pre-freezing on motility, viability and abnormality of Nile tilapia fish Oreochromis niloticus sperm post cryopreservation

• Autorzy: Muchlisin Z.A. , Hasibuan R.R. , Maulida S. , Eriani K. , Fadli N. , Muhammadar A.A. , Handayani L.S. , Suharman I. , Kocabas M. , Kocabas F.K.
• Języki publikacji: EN

Abstrakty

EN

Nile tilapia Oreochromis niloticus is a popular freshwater fish that has been extensively and intensively cultured worldwide. However, cryopreservation of its sperm, especially pre-freezing procedure, has not been properly developed. Therefore, the study aimed to determine the best pre-freezing procedure for cryopreservation of Nile tilapia Oreochromis niloticus sperm. The completely randomized design with five treatments and four replications was employed in this study. The tested treatments were T1=4°C → 0°C → -4°C → -10°C → -79°C → -196°C, T2=4°C→ 0°C → -4°C → -10°C → -196°C, T3=4°C → 0°C → -4°C → -196°C, T4=4°C → 0°C → -196°C), and T5=4°C → -196°C, with a 10 min equilibration at each respective temperature. Furthermore, sperm was cryopreserved for two weeks in liquid nitrogen (-179 oC). The results of the ANOVA test showed that pre-freezing had a significant effect on sperm motility, and viability (P<0.05), but had no considerable impact on sperm abnormality (P>0.05). Treatment T4 exhibited higher motility and viability, but these values were not significantly different from T3 and T5. Based on practical consideration, it was recommended to utilize the T5 pre-freezing procedures (4°C → -196°C) for cryopreservation of Nile tilapia sperm. Considering these results, Nile tilapia sperm can be directly cryopreserved into liquid nitrogen after equilibration at 4°C for 10 min.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2025
• Tom: 28
• Numer: 1
• Opis fizyczny: p.150-164,ref.

Twórcy

autor

Muchlisin Z.A.

• Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
• Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

autor

Hasibuan R.R.

• Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

autor

Maulida S.

• Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
• Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

autor

Eriani K.

• Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

autor

Fadli N.

• Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

autor

Muhammadar A.A.

• Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

autor

Handayani L.S.

• Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

autor

Suharman I.

• Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Universitas Riau, Pekanbaru, Indonesia

autor

Kocabas M.

• Faculty of Life Sciences, Karadeniz Technical University, Trabzon, Turkey

autor

Kocabas F.K.

• Faculty of Fisheries, Munzur University, Turkey

Identyfikatory

DOI

10.24425/pjvs.2025.154024