Main centres of nitrate and nitrite reduction in young and nodulated yellow lupine (Lupinus luteus)

• Autorzy: Polcyn W. , Lucinski R.
• Języki publikacji: EN

Abstrakty

EN

Nitrate and nitrite reduction centers in nonnodulated and symbiotic yellow lupine were analyzed. In young seedlings, nitrate was exclusively accumulated in roots, which also was shown as the main nitrate reduction center. In contrast, leaves were shown to play a key role in nitrite reduction. A similar distribution of nitrate reductase (NR) and nitrite reductase was found in nodulated plants. However, in field conditions characterized by low nitrate content, a disproportionately high level of NR activity in nodules was also observed during all stages of symbiotic growth. This feature was confirmed in nitrate-fed hydroponic cultures. Nodule NR activity was one order of magnitude higher than in roots, in spite of the small stored nitrate pool found inside nodules. This suggests that nodule NR activity had been induced not by nitrate itself but indirectly. Since bacteroids were shown to be responsible for the vast majority of nodule NR activity, the plausible explanation of this effect seems to be a dissimilatory nature of rhizobial NR. Considering that environmental nitrate could cause hypoxia inside nodules, this is the proposed way of the observed nodule NR induction.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 3
• Opis fizyczny: p.605-610,fig.,ref.

Twórcy

autor

Polcyn W.

• Institute of Experimental Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland

autor

Lucinski R.

• Institute of Experimental Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland

Bibliografia

• Atkins CA, Pate JS et al (1980) Economy of carbon and nitrogen in nodulated and nonnodulated (NO₃-grown) cowpea (Vigna unguiculata (L.) Walp). Plant Physiol 66:978–983
• Becana M, Sprent JI (1987) Nitrogen fixation and nitrite reduction in the root nodules of legumes. Physiol Plant 70:757–765. doi: 10.1111/j.1399-3054.1987.tb04335.x
• Emes MJ, Bowsher CG (1991) Integration and compartmentation of carbon and nitrogen metabolism in roots. In: Emes MJ (ed) Compartmentation of plant metabolism in non-photosynthetic tissues. Cambridge University Press, New York, pp 147–165
• Heller R (1954) Recherches sur la nutrition minerale des tissus vegetaux cultives in vitro. Ann Sc Nat Bot Biol Veg 14:1–7
• Hunter WJ (1983) Soybean root and nodule nitrate reductase. Physiol Plant 59:471–475. doi:10.1111/j.1399-3054.1983.tb04232.x
• Lillo C, Meyer C et al (2004) Mechanism and importance of posttranslational regulation of nitrate reductase. J Exp Bot 55:1275–1282. doi:10.1093/jxb/erh132
• Luciński R, Polcyn W et al (2002) Nitrate reduction and nitrogen fixation in symbiotic association Rhizobium—legumes. Acta Biochim Pol 49:537–546
• Pate JS, Layzell DB et al (1979) Economy of C and N in a nodulated and non-nodulated (NO₃-grown) legume. Plant Physiol 64:1083–1088
• Polcyn W, Luciński R (2001) Functional similarities of nitrate reductase from yellow lupine bacteroids to bacterial denitrification systems. J Plant Physiol 158:829–834. doi:10.1078/0176-1617-00271
• Polcyn W, Luciński R (2003) Aerobic and anaerobic nitrate reduction in free-living cells of Bradyrhizobium sp. (Lupinus). FEMS Microbiol Lett 226:331–337. doi:10.1016/S0378-1097(03) 00620-7
• Polcyn W, Luciński R (2006) Dissimilatory nitrate reductase from Bradyrhizobium sp. (Lupinus): subcellular location, catalytic properties and characterization of the active enzyme forms. Curr Microbiol 52:231–237. doi:10.1007/s00284-005-0265-x
• Redinbaugh MG, Campbell WH (1991) Higher plant responses to environmental nitrate. Physiol Plant 82:640–650. doi:10.1111/j.1399-3054.1991.tb02958.x
• Senn DR, Carr PW et al (1976) Minimization of a sodium dithionitederived interference in nitrate reductase–methyl viologen reactions. Anal Biochem 75:464–471. doi:10.1016/0003-2697(76) 90101-9
• Somasegaran P, Hoben HJ (1994) Handbook for rhizobia: methods in legume-rhizobium technology. Springer, New York, pp 366–369
• Vincill ED, Szczygłowski K et al (2005) GmN70 and LjN70. Anion transporters of the symbiosome membrane of nodules with a transport preference for nitrate. Plant Physiol 137:1435–1444. doi:10.1104/pp.104.051953
• Wallace W (1986) Distribution of nitrate assimilation between the root and shoot of legumes and a comparison with wheat. Physiol Plant 66:630–636. doi:10.1111/j.1399-3054.1986.tb05591.x

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