Functional analyses of the maize CKS2 gene promoter in response to abiotic stresses and hormones

• Autorzy: Wang F. , Liu J. , Li J. , Zhang S. , Pan H.
• Języki publikacji: EN

Abstrakty

EN

In our previous research, we showed that the cyclin-dependent kinase regulatory subunit (CKS2) in maize (Zea mays L.) was induced by water deficit and cold stress. To elucidate its expression patterns under adversity, we isolated and characterized its promoter (PZmCKS2). A series of PZmCKS2-deletion derivatives, P0–P3, from the translation start code (-1,455, -999, -367, and -3 bp) was fused to the β-glucuronidase (GUS) reporter gene, and each deletion construct was analyzed by Agrobacterium-mediated steady transformation into Arabidopsis. Leaves were then subjected to dehydration, cold, abscisic acid (ABA), salicylic acid (SA), and methyl jasmonic acid (MeJA). Sequence analysis showed that several stressrelated cis-acting elements (MBS, CE3, TGA element, and ABRE) were located within the promoter. Deletion analysis of the promoter, PZmCKS2, suggested that the -999 bp promoter region was required for the highest basal expression of GUS, and the -367 bp sequence was the minimal promoter for ZmCKS2 activation by low temperature, ABA, and MeJA. The cis-acting element ABRE was necessary for promoter activation by exogenous ABA.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 07
• Opis fizyczny: p.1867-1878,fig.,ref.

Twórcy

autor

Wang F.

• College of Plant Sciences, Jilin University, 130062 Changchun, China

autor

Liu J.

• College of Plant Sciences, Jilin University, 130062 Changchun, China

autor

Li J.

• College of Plant Sciences, Jilin University, 130062 Changchun, China

autor

Zhang S.

• College of Plant Sciences, Jilin University, 130062 Changchun, China

autor

Pan H.

• College of Plant Sciences, Jilin University, 130062 Changchun, China

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Identyfikatory

DOI

10.1007/s11738-014-1563-3