Heptoprotective role of Artemisia scoparia Waldst. and kit against CCl4-induced toxicity in rats

• Autorzy: Waris T.S. , Khan M.R. , Shah N.A. , Shuaib M. , Hussain F. , Ishaq M. , Kamal Z. , Zeb U. , Ali S. , Ahmed S. , Hussain F.
• Języki publikacji: EN

Abstrakty

EN

In this study, the methanol extract of Artemisia scoparia was evaluated for its protective potential against carbon tetrachloride (CCl₄)-induced hepatic toxicity. Seven groups of mature albino rats were used in the course of the experiment and each group was treated with specific doses of plant extract and CCl₄. Silymarin was used as a standard protective drug. The results of the experiment revealed that Artemisia scoparia plant extract was successful in fighting CCl₄ toxicity as it clearly reduced the elevated levels of liver serum markers (alkaline phosphatase and alkaline aminotransferase), lipid peroxidation, nitrite content, and H₂O₂ on one side while enhancing the levels of antioxidant enzymes (catalase, peroxidase, superoxide dismutase, glutathione-s-transferase, γ-Glutamyltranspeptidase, and glutathione reductase) and protein content. It also protected DNA from the damaging effects of CCl₄. The findings of this study demonstrate that Artemisia scoparia plant extract plays a significant role in preventing the hepatic damages instigated with CCl₄ and can be used as a protective agent against oxidative stress-associated disorders.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2018
• Tom: 27
• Numer: 3
• Opis fizyczny: p.1307-1314,fig.,ref.

Twórcy

autor

Waris T.S.

• Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

autor

Khan M.R.

• Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

autor

Shah N.A.

• Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan

autor

Shuaib M.

• School of Ecology and Environmental Science, Yunnan University, No. 2 North Cuihu Road, Kunming, Yunnan, 650091, P.R. China

autor

Hussain F.

• Yunnan institute of Microbiology, School of Life Science, Yunnan University, Kunming, 650091, P.R. China

autor

Ishaq M.

• School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, District Minhang Shanghai, China

autor

Kamal Z.

• School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, District Minhang Shanghai, China
• Shaheed Benazir Bhutto University, Sheringal, KP, Pakistan

autor

Zeb U.

• 7 Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xian 710069, P.R. China

autor

Ali S.

• Department of Botany, Bacha Khan University Charsadda, Pakistan

autor

Ahmed S.

• Key Laboratory of Molecular Epigenetics, Ministry of Education, School of Life Sciences, Northeast Normal University, Changchun, 130024, Jilin, P.R. China

autor

Hussain F.

• 1School of Resources, Environmental and Chemical Engineering Nanchang University, Nanchang Jiangxi, P.R. China

Bibliografia

• 1. VALKO M., RHODES C.J., MONCOL J., IZAKOVIC M., MAZUR M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem. Biol. Interact. 160, (1), 1-40, 2006.
• 2. SAJID M., KHAN M.R., SHAH N.A., ULLAH S., YOUNIS T., Majid M. Proficiencies of Artemisia scoparia against CCl₄ induced DNA damages and renal toxicity in rat. BMC Complement Altern Med.16 (1), 149, 2016.
• 3. ALKREATHY H.M., KHAN R.A., KHAN M.R., SAHREEN S. CCl₄ induced genotoxicity and DNA oxidative damages in rats: hepatoprotective effect of Sonchus arvensis. BMC Complement Altern Med. 14 (1), 452, 2014.
• 4. SHAH N.A., KHAN M.R. Antidiabetic effect of Sida cordata in alloxan induced diabetic rats. BioMed Res. Int. 2014.
• 5. KHAN M.R., RIZVI W., KHAN G.N., KHAN R.A., SHAHEEN S. Carbon tetrachloride-induced nephrotoxicity in rats: Protective role of Digera muricata. J Ethnopharmacol. 122 (1), 91, 2009.
• 6. HAYAT M.Q., KHAN M.A., ASHRAF M., JABEEN S. Ethnobotany of the genus Artemisia L.(Asteraceae) in Pakistan. 2009.
• 7. AHMAD S.S., JAVED S. Exploring the economic value of underutilized plant species in Ayubia National Park. Pak J Bot. 39 (5), 1435, 2007.
• 8. SINGH H.P., MITTAL S., KAUR S., BATISH D.R., KOHLI R.K. Chemical composition and antioxidant activity of essential oil from residues of Artemisia scoparia. Food chemi. 114 (2), 642, 2009.
• 9. IBRAR M., HUSSAIN F. Ethnobotanical studies of plants of Charkotli hills, Batkhela district, Malakand, Pakistan. Front Biolin China. 4 (4), 539, 2009.
• 10. SAHREEN S., KHAN M.R., KHAN R.A. Hepatoprotective effects of methanol extract of Carissa opaca leaves on CCl₄-induced damage in rat. BMC Complement Altern Med. 11 (1), 48, 2011.
• 11. KADIISKA M.B., GLADEN B.C., BAIRD D.D., GERMOLEC D., GRAHAM L.B., PARKER C.E. Biomarkers of Oxidative Stress Study II: Are oxidation products of lipids, proteins, and DNA markers of CCl₄ poisoning? Free RadicBiol Med. 38 (6), 698, 2005.
• 12. KHAN R.A., KHAN M.R., SAHREEN S., SHAH N.A., BOKHARI J., SHABBIR M. Protective effects of Launaea procumbens against KBrO₃-induced hepatic serum marker enzymes. Afr. J. Pharm. Pharmacol. 5 (23), 2639, 2011.
• 13. GUHA G., VENKATADRI R., MATHEW L., RANGASAMY A.K. The antioxidant and DNA protection potential of Indian tribal medicinal plants. Turkish J. Biol. 35 (2), 233, 2011.
• 14. KHAN R.A., KHAN M.R., SHAH N.A., SAHREEN S., SIDDIQ P. Modulation of carbon tetrachloride-induced nephrotoxicity in rats by n-hexane extract of Sonchus asper. ToxicolInd Health. 31 (10), 955, 2015.
• 15. KANTER M., MERAL I., DEDE S., CEMEK M., OZBEK H., UYGAN I. Effects of Nigella sativa L. and Urtica dioica L. on Lipid Peroxidation, Antioxidant Enzyme Systems and Some Liver Enzymes in CCl₄-Treated Rats. J Vet Med A. 50 (5), 264, 2003.
• 16. NAZ K., KHAN M.R., SHAH N.A., SATTAR S., NOUREEN F., AWAN M.L. Pistacia chinensis: A potent ameliorator of CCl₄ induced lung and thyroid toxicity in rat model. BioMed Res. Int. 2014.
• 17. KHAN R.A., KHAN M.R., SAHREEN S., AHMED M., SHAH N.A. Carbon tetrachloride-induced lipid peroxidation and hyperglycemia in rat: a novel study. ToxicolInd Health. 31 (6), 546, 2015.
• 18. KAKKAR P., DAS B., VISWANATHAN P.N. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys. 21 (2), 130, 1984.
• 19. SHAH N.A., KHAN M.R. Increase of glutathione, testosterone and antioxidant effects of Jurenia dolomiaea on CCl₄ induced testicular toxicity in rat. BMC Complement Altern Med. 17 (1), 206, 2017.
• 20. SAJID M., KHAN M.R., SHAH N.A., SHAH S.A., ISMAIL H., YOUNIS T. Phytochemical, antioxidant and hepatoprotective effects of Alnus nitida bark in carbon tetrachloride challenged Sprague Dawley rats. BMC Complement Altern Med. 16 (1), 268, 2016.
• 21. AHMAD B., KHAN M.R., SHAH N.A. Amelioration of carbon tetrachloride-induced pulmonary toxicity with Oxalis corniculata. ToxicolInd Health. 31 (12), 1243, 2015.
• 22. DHAWAN A., BAJPAYEE M.M., PANDEY A.K., PARMAR D. Protocol for the single cell gel electrophoresis/comet assay for rapid genotoxicity assessment. Sigma. 1077, 1, 2009.
• 23. GILANI A-uH., JANBAZ K.H. Protective effect of Artemisia scoparia extract against acetaminophen-induced hepatotoxicity. Gen.Pharmacol-Vasc. S. 24 (6), 1455, 1993.
• 24. SINGH H.P., MITTAL S., KAUR S., BATISH D.R., KOHLI R.K. Chemical composition and antioxidant activity of essential oil from residues of Artemisia scoparia. Food Chem. 114 (2), 642, 2009.
• 25. XIAO Z.P., WANG X.D., PENG Z.Y., HUANG S., YANG P., LI Q.S. Molecular Docking, Kinetics Study, and Structure-Activity Relationship Analysis of Quercetin and Its Analogous as Helicobacter pylori Urease Inhibitors. J. Agric. Food Chem. 60 (42), 10572, 2012.
• 26. MELIN A.M., PERROMAT A., DÉLÉRIS G. Pharmacologic application of Fourier transform IR spectroscopy: in vivo toxicity of carbon tetrachloride on rat liver. Biopolym. 57 (3), 160, 2000.
• 27. PRAVEEN T.K., DHARMARAJ S., BAJAJ J., DHANABAL S.P., MANIMARAN S., NANJAN M.J. Hepatoprotective activity of petroleum ether, diethyl ether, and methanol extract of Scoparia dulcis L. against CCl₄-induced acute liver injury in mice. Indian J. Pharmacol. 41 (3), 110, 2009.
• 28. AZQUETA A., COLLINS A.R. The essential comet assay: a comprehensive guide to measuring DNA damage and repair. Arch Toxicol. 87 (6), 949, 2013

Identyfikatory

DOI

10.15244/pjoes/74018