Screening of genes encoding isoforms of lectin in Japanese chestnut (Castanea crenata) trees

• Autorzy: Nomura K. , Ikegami A. , Nakamura Y.
• Języki publikacji: EN

Abstrakty

EN

Screening of a cDNA library revealed that Castanea crenata agglutinin (CCA) consists of two homologous protomers: CCA, which has been reported previously, and its isoform, designated CCA-Q, which contains an additional glutamine residue inserted at position 153 of CCA. PCR-Southern and subsequent analyses of the genomic sequence indicated that both CCA and CCA-Q should be translated from the same gene, CCA, which consists of four exons and three introns. Therefore, the difference may be caused by a type of alternative splicing. PCR-Southern analysis also indicated the existence of another gene, sCCA, which showed high identity to exon 1, intron 1 and exon 4 of CCA. In addition, the entire sCCA gene was transcribed into an mRNA with no obvious open reading frame, although the amount of transcribed product was less than 1/100 of the level of the CCA transcript. Thus, sCCA is estimated to be a pseudogene. These results suggest that no CCA isoforms encoded by different genes are present in Japanese chestnut trees, except for the presence of a homolog pseudogene.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2008
• Tom: 30
• Numer: 2
• Opis fizyczny: p.175-181,fig.,ref.

Twórcy

autor

Nomura K.

• Department of Plant Resource Science, Faculty of Agriculture, Kobe University, 1-1 Rokkodai-cho, Nada-ku, 657-8501 Kobe, Japan

autor

Ikegami A.

• Department of Plant Resource Science, Faculty of Agriculture, Kobe University, 1-1 Rokkodai-cho, Nada-ku, 657-8501 Kobe, Japan

autor

Nakamura Y.

• Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai-cho, Nada-ku, 657-8501 Kobe, Japan

Bibliografia

• Chisholm ST, Mahajan SK, Whitham SA, Yamamoto ML, Carrington JC (2000) Cloning of the Arabodopsis RTM1 gene, which controls restriction of long-distance movement of tobacco etch virus. Proc Natl Acad Sci USA 97:489–494
• Eddy SR (2001) Non-coding RNA genes and the modern RNA world. Nature Rev Genet 2:919–929
• Erdmann VA, Szymanski M, Hochberg A, de Groot N, Barciszewski J (1999) Collection of mRNA-like non-coding RNAs. Nucleic Acids Res 27:192–195
• Hirano K, Teraoka T, Yamanaka H, Harashima A, Kunisaki A, Takahashi H, Hosokawa D (2000) Novel mannose-binding rice lectin composed of some isolectins and its relation to a stressinducible salT gene. Plant Cell Physiol 41:258–267
• Mann K, Farias CMSA, Del Sol FG, Santos CF, Grangerio TB, Nagano CS, Cavada BS, Calvete JJ (2001) The amino-acid sequence of the glucose/mannose-specific lectin isolated from Parkia platycephala seeds reveals three tandemly arranged jacalin-related domains. Eur J Biochem 268:4414–4422
• Nakamura S, Ikegami A, Mizuno M, Yagi F, Nomura K (2004) The expression profile of lectin differs from that of seed storage proteins in Castanea cranata trees. Biosci Biotechnol Biochem 68:1689–1705
• Nomura K, Ashida H, Uemura N, Kushibe S, Ozaki T, Yoshida M (1998) Purification and characterization of a mannose/glucosespecific lectin from Castanea crenata. Phytochemistry 49:667–673
• Nomura K, Nakamura S, Fujitake M, Nakanishi T (2000) Compete amino acid sequence of Japanese chestnut agglutinin. Biochem Biophys Res Commun 276:23–28
• Peumans WJ, Winter HC, Bemer V, Van Leuven F, Goldstein IJ, Truffa-Bachi P, Van Damme EJM (1999) Isolation of a novel plant lectin with an unusual specificity from Calystegia sepium. Glycoconjugate J 14:259–265
• Peumans WJ, Zhang W, Barre A, Astoul CH, Balint-Kurti PJ, Rovira P, Roungé P, May GD, Leuven FV, Truffa-Bachi P, Van Damme EJM (2000a) Fruit-specific lectins from banana and plantain. Planta 211:546–554
• Peumans WJ, Hause B, Van Damme EJM (2000b) The galactosebinding and mannose-binding jacalin-related lectins are located in different sub-cellular compartments. FEBS Lett 477:186–192
• Raval S, Gowda SB, Singh DD, Chandra NR (2004) A database analysis of jacalin-related lectins: sequence- structure-function relationships. Glycobiology 14:12247
• Smith CWJ, Patton JG, Nadal-Ginard B (1989) Alternative splicing in the control of gene expression. Annu Rev Genet 23:527–577
• Taipalensuu J, Eriksson S, Rask L (1997) The myrosinase-binding protein from Brassica napus seeds possesses lectin activity and has a highly similar vegetatively expressed wound-inducible counterpart. Eur J Biochem 250:680–688
• Tateno H, Winter HC, Petryniak J, Goldstein IJ (2003) Purification, characterization, molecular cloning, and expression of novel members of jacalin-related lectins from rhizomes of the true fern Phlebodium aureum (L) J. Smith (Polypodiaceae). J Biol Chem 278:10891–10899
• Van Damme EJM, Barre A, Mazard A-M, Verhaert P, Horman A, Debray H, Rougé P, Peumans WJ (1999) Characterization and molecular cloning of the lectin from Helianthus tuberosus. Eur J Biochem 259:135–142
• Van Damme EJM, Hause B, Hu J, Barre A, Rougé P, Proosr P, Peumans WJ (2002) Two distinct Jacalin-related lectins with a different specificity and subcellular location are major vegetative storage proteins in the bark of black mulberry tree. Plant Physiol 130:757–769
• Yagi F, Iwaya T, Haraguchi T, Goldstein IJ (2002) The lectin from leaves of Japanese cycad, Cycas revolute Thunb. (gymnosperm) is a member of the jacalin-related family. Eur J Biochem 269:4335–4341
• Yang H, Czapla TH (1993) Isolation and characterization of cDNA clones encoding Jacalin isolectins. J Biol Chem 268:5905–5910a

Uwagi

Rekord w opracowaniu