Callus-mediated and direct protocorm-like body formation of Bletilla striata and assessment of clonal fidelity using ISSR markers

• Autorzy: Wang C.X. , Tian M.
• Języki publikacji: EN

Abstrakty

EN

Two efficient morphogenetic pathways for micropropagation of Bletilla striata (Thunb.) Reichb. f. have been established through the callus-mediated and direct formation of protocorm-like bodies (PLBs) from protocorms and shoot tips. Green calli were induced from the basal surface of protocorms and the cut-end of shoot tips on Vacin and Went (VW) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthalene acetic acid (NAA) after 3–5 weeks, with the highest frequency of explants forming callus (48.0 %) from protocorms at 1.0 mg l⁻¹ 2,4-D. The calli obtained from all plant growth regulator (PGR) treatments could proliferate and differentiate PLBs on the PGR-free medium. NAA and 2,4-D significantly enhanced the growth of callus. The fastest growth rate of callus was achieved at the combination of 1.0 mg l⁻¹ 2,4-D and 1.0 mg l⁻¹ TDZ with 46.2- fold within 3 months. The regeneration of PLBs from callus was significantly improved by 6-benzyladenine (BA), and a mean number of 48.4 PLBs was produced from 100 mg calli at 1.0 mg l⁻¹ BA within 3 months. BA and thidiazuron (TDZ) promoted the direct formation of PLBs from explants. The highest frequency of direct PLBs formation (76.0 %) and the highest mean number of PLBs per explant (30.2) were observed in protocorms cultured with 0.5 mg l⁻¹ BA. Assessment of clonal fidelity by intersimple sequence repeat (ISSR) markers revealed similarity ranges of 99.8–100.0 % between the regenerants and their mother plants and 99.5–100.0 % among the regenerants, which suggested the micropropagation protocols were genetically stable.

Słowa kluczowe

EN

Bletilla striata; callus; micropropagation; protocorm-like body; formation; assessment; clonal fidelity; ISSR marker

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 09
• Opis fizyczny: p.2321-2330,fig.,ref.

Twórcy

autor

Wang C.X.

• Research Institute of Subtropical Forestry, Chinese Academy of Forestry, 311 400 Fuyang, Zhejiang, China

autor

Tian M.

• Research Institute of Subtropical Forestry, Chinese Academy of Forestry, 311 400 Fuyang, Zhejiang, China

Bibliografia

• Al-Zahim MA, Ford-Lloyd BV, Newbury HJ (1999) Detection of somaclonal variation in garlic (Allium sativum L.) using RAPD and cytological analysis. Plant Cell Rep 18:473–477
• Bairu MW, Aremu AO, Staden JV (2011) Somaclonal variation in plants: causes and detection methods. Plant Growth Regul 63:147–173
• Chen JT, Chang WC (2000) Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium (Orchidaceae). Plant Sci 160:87–93
• Chen JT, Chang WC (2004a) TIBA affects the induction of direct somatic embryogenesis from leaf explants of Oncidium. Plant Cell Tissue Organ Cult 79:315–320
• Chen JT, Chang WC (2004b) Induction of repetitive embryogenesis from seed-derived protocorms of Phalaenopsis amabilis var. Formosa shimadzu. In Vitro Cell Dev Biol Plant 40:290–293
• Chen JT, Chang WC (2006) Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis. Biol Plantarum 50:169–173
• Chugh S, Guha S, Rao IU (2009) Micropropagation of orchids: a review on the potential of different explants. Sci Hortic 122:507–520
• Chung MY, Chung MG (2005) Pollination biology and breeding systems in the terrestrial orchid Bletilla striata. Plant Syst Evol 252:1–9
• Cooper C, Crowther T, Smith BM, Isaac S, Collin HA (2006) Assessment of the response of carrot somaclones to Pythium violae, causal agent of cavity spot. Plant Pathol 55:427–432
• Dhiman A, Pathak P, Vij SP (2007) Regeneration from foliar explants in Bletilla striata Reichb. f.: a study in vitro. J Orchid Soc India 21:75–80
• Diao H, Li X, Chen J, Luo Y, Chen X, Dong L, Wang C, Zhang C, Zhang J (2008) Bletilla striata polysaccharide stimulates inducible nitric oxide synthase and proinflammatory cytokine expression in macrophages. J Biosci Bioeng 105:85–89
• Feng JQ, Zhang RJ, Zhao WM (2008) Novel bibenzyl derivatives from the tubers of Bletilla striata. Helv Chim Acta 91:520–525
• Huan LVT, Takamura T, Tanaka M (2004) Callus formation and plant regeneration from callus through somatic embryo structures in Cymbidium orchid. Plant Sci 166:1443–1449
• Huang CH, Chung JP (2011) Efficient indirect induction of protocorm-like bodies and shoot proliferation using field-grown axillary buds of a Lycaste hybrid. Plant Cell Tissue Organ Cult 106:31–38
• Jheng FY, Do YY, Liauh YW, Chung JP, Huang PL (2006) Enhancement of growth and regeneration efficiency from embryogenic callus cultures of Oncidium ‘Gower Ramsey’ by adjusting carbohydrate sources. Plant Sci 170:1133–1140
• Khoddamzadeh AA, Sinniah UR, Kadir MA, Kadzimin SB, Mahmood M, Sreeramanan S (2011) In vitro induction and proliferation of protocorm-like bodies (PLBs) from leaf segments of Phalaenopsis bellina (Rchb.f.) Christenson. Plant Growth Regul 65:381–387
• Lee YI, Lee N (2003) Plant regeneration from protocorm-derived callus of Cypripedium formosanum. In Vitro Cell Dev Biol Plant 39:475–479
• Lee YI, Hsu ST, Yeung EC (2013) Orchid protocorm-like bodies are somatic embryos. Am J Bot 100:2121–2131
• Li Y, Xu C, Zhang Q, Liu JY, Tan RX (2005) In vitro anti-Helicobacter pylori action of 30 Chinese herbal medicines used to treat ulcer diseases. J Ethnopharmacol 98:329–333
• Lin YH, Chang C, Chang WC (2000) Plant regeneration from callus culture of a Paphiopedilum hybrid. Plant Cell Tissue Organ Cult 62:21–25
• Morita H, Koyama K, Sugimoto Y, Kobayashi J (2005) Antimitotic activity and reversal of breast cancer resistance protein-mediated drug resistance by stilbenoids from Bletilla striata. Bioorg Med Chem Lett 15:1051–1054
• Neelakandan AK, Wang K (2012) Recent progress in the understanding of tissue culture-induced genome level changes in plants and potential applications. Plant Cell Rep 31:597–620
• Qian J, Vossoughi D, Woitaschek D, Oppermann E, Bechstein WO, Li WY, Feng GS, Vogl T (2003) Combined transarterial chemoembolization and arterial administration of Bletilla striata in treatment of liver tumor in rats. World J Gastroenterol 9:2676–2680
• Rathore MS, Chikara J, Mastan SG, Rahman H, Anand KG, Shekhawat NS (2011) Assessment of genetic stability and instability of tissue culture-propagated plantlets of Aloe vera L. by RAPD and ISSR markers. Appl Biochem Biotech 165:1356–1365
• Rohlf FJ (2000) NTSYS-pc: numerical taxonomy and multivariate analysis system. Exeter Software, New York
• Roy J, Naha S, Majumdar M, Banerjee N (2007) Direct and callus-mediated protocorm-like body induction from shoot-tips of Dendrobium chrysotoxum Lindl. (Orchidaceae). Plant Cell Tissue Organ Cult 90:31–39
• Sugiura N (1995) The pollination ecology of Bletilla striata (Orchidaceae). Ecol Res 10:171–177
• Teixeira da Silva JA, Singh N, Tanaka M (2006) Priming biotic factors for optimal protocorm-like body and callus induction in hybrid Cymbidium (Orchidaceae), and assessment of cytogenetic stability in regenerated plantlets. Plant Cell Tissue Organ Cult 84:135–144
• Vacin EF, Went FW (1949) Some pH changes in nutrient solutions. Bot Gaz 110:605–613
• Wang C, Sun J, Luo Y, Xue W, Diao H, Dong L, Chen J, Zhang J (2006) A polysaccharide isolated from the medicinal herb Bletilla striata induces endothelial cells proliferation and vascular endothelial growth factor expression in vitro. Biotechnol Lett 28:539–543
• Wu IF, Chen JT, Chang WC (2004) Effects of auxins and cytokinins on embryo formation from root-derived callus of Oncidium ‘Gower Ramsey’. Plant Cell Tissue Organ Cult 77:107–109
• Zhao P, Wu F, Feng FS, Wang WJ (2008) Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl. In Vitro Cell Dev Biol Plant 44:178–185

Identyfikatory

DOI

10.1007/s11738-014-1594-9