Salt tolerance analysis of Arabidopsis thaliana NOK2 accession under saline conditions and potassium supply

• Autorzy: Kaddour R. , Baatour O. , Mahmoudi H. , Nasri N. , Zaghdoudi M. , Lachaal M.
• Języki publikacji: EN

Abstrakty

EN

The response to salt treatment and K⁺ provision of two Arabidopsis thaliana accessions grown for 17 days in the presence of 50 mM NaCl was investigated. Leaf and root dry weight deposition was restricted by salt, more in Col accession than in NOK2 accession. In both accessions, the growth inhibition induced by salinity was associated with a decrease in total leaf surface area, which resulted from diminished leaf number, but not from restriction of individual leaf surface area. Comparing the effects of salt on dry matter production and total leaf surface area revealed large difference between Col and NOK2 for net assimilation rate (the amount of whole plant biomass produced per unit leaf surface area), which was augmented by salt and K⁺ in NOK2 but not in Col. This result, which suggested a better capacity of NOK2 to preserve its photosynthetic machinery against salt stress, was in agreement with the effect of NaCl on photosynthetic pigments. Indeed, salt significantly reduced chlorophyll and carotenoid content in Col leaves but had no impact on NOK2 leaf pigment content. Since K⁺ provision had only marginal effects on these responses to salt stress, leaf mineral unbalance was unlikely. Guaiacol peroxidase activity was augmented by salt treatment in leaves and roots of both accessions. Salinity decreased the catalase activity in Col leaves and in roots, and increased this activity in NOK2 organs. In conclusion, when aggressed by salt, NOK2 was able (1) to produce more leaves than Col, and (2) to efficiently protect its photosynthetic apparatus, perhaps by developing more efficient antioxidative defense through increased catalase and peroxidase activities. Consequently, the overall photosynthetic activity was higher and more robust to salt aggression in NOK2 than in Col.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 6
• Opis fizyczny: p.2083-2090,fig.,ref.

Twórcy

autor

Kaddour R.

• Physiologie et Biochimie de la Torerance au Sel des Plantes, Faculte des Sciences de Tunis, Campus Universitaire, 1060 Tunis, Tunisia

autor

Baatour O.

• Physiologie et Biochimie de la Torerance au Sel des Plantes, Faculte des Sciences de Tunis, Campus Universitaire, 1060 Tunis, Tunisia

autor

Mahmoudi H.

• Physiologie et Biochimie de la Torerance au Sel des Plantes, Faculte des Sciences de Tunis, Campus Universitaire, 1060 Tunis, Tunisia

autor

Nasri N.

• Physiologie et Biochimie de la Torerance au Sel des Plantes, Faculte des Sciences de Tunis, Campus Universitaire, 1060 Tunis, Tunisia

autor

Zaghdoudi M.

• Physiologie et Biochimie de la Torerance au Sel des Plantes, Faculte des Sciences de Tunis, Campus Universitaire, 1060 Tunis, Tunisia

autor

Lachaal M.

• Physiologie et Biochimie de la Torerance au Sel des Plantes, Faculte des Sciences de Tunis, CampusUniversitaire, 1060 Tunis, Tunisia

Bibliografia

• Amtmann A, Hammond JP, Armengaud P, White PJ (2006) Nutrient sensing and signalling in plants: potassium and phosphorus. Adv Bot Res 43:209–257
• Attia H, Arnaud N, Karray N, Lachaâl M (2008a) Long-term effects of mild salt stress on growth, ion accumulation and superoxide dismutase expression of Arabidopsis rosette leaves. Physiol Plant 132:293–305
• Attia H, Karray N, Rabhi M, Lachaâl M (2008b) Salt-imposed restrictions on the uptake of macroelements by roots of Arabidopsis thaliana. Acta Physiol Plant 30:723–727
• Attia H, Karray N, Lachaâl M (2009) Light interacts with salt stress in regulating superoxide dismutase gene expression in Arabidopsis. Plant Sci 177:161–167
• Bartels D, Sunkar R (2005) Drought and salt tolerance in plants. CRC Crit Rev Plant Sci 24:23–58
• Benlloch M, Ojeda MA, Ramos J, Rodriguez-Navarro A (1994) Salt sensitivity and low discrimination between potassium and sodium in bean plants. Plant Soil 166:117–123
• Botella MA, Martinez V, Pardines J, Cerda A (1997) Salinity induced potassium deficiency in maize plants. J Plant Physiol 150: 200–205
• Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
• Cakmak I (2005) The role of potassium in alleviating detrimental effects of abiotic stresses in plants. J Plant Nutr Soil Sci 168:521–530
• Carden DE, Walker DJ, Flowers TJ, Miller AJ (2003) Single cell measurements of the contributions of cytosolic Na⁺ and K⁺ to salt tolerance. Plant Physiol 131:676–683
• Chance B, Maehly SK (1955) Assay of catalase and peroxidases. Method Enzymol 2:764–775
• De Pascale S, Maggio A, Fogliano V, Ambrosino P, Ritieni A (2001) Irrigation with saline water improves carotenoids content and antioxidant activity of tomato. J Hort Sci Biotechnol 76:447–453
• Delgado IC, Sanchez-Raya AJ (1999) Physiological response of sunflower seedlings to salinity and potassium supply. Commun Soil Sci Plant Anal 30:773–783
• Ding YC, Chang CR, Luo W, Wu YS, Ren XL, Wang P, Xu GH (2008) High potassium aggravates the oxidative stress induced by magnesium deficiency in rice leaves. Pedosphere 18:316–327
• El-Hendawy SE, Hu Y, Schmidhalter U (2005) Growth, ion content, gas exchange, and water relations of wheat genotypes differing in salt tolerances. Aust J Agric Res 56:123–134
• Fielding JL, Hall JA (1978) Biochemical and cytochemical study of peroxidase activity in roots of Pisum Sativum. J Exp Bot 29:969–981
• Gay AP, Hauck B (1994) Acclimation of Lolium temulentum to enhanced carbon dioxide concentration. J Exp Bot 45:1133–1141
• Ghars MA, Parre E, Debez A, Bordenave M, Richard L, Leport L, Bouchereau A, Savouré A, Abdelly C (2008) Comparative salt tolerance analysis between Arabidopsis thaliana and Thellungiella halophila, with special emphasis on K⁺/Na⁺ selectivity and proline accumulation. J Plant Physiol 165:588–599
• Golldack D, Quigley F, Michalowski CB, Kamasani UR, Bohnert HJ (2003) Salinity stress-tolerant and -sensitive rice (Oryza sativa L.) regulate AKT1-type potassium channel transcripts differently. Plant Mol Biol 51:71–81
• Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ (2000) Plant cellular and molecular responses to high salinity. Annu Rev Plant Physiol Plant Mol Biol 51:463–499
• Hunt R (1990) Basic growth analysis. Unwin, Hyman, London, p 112
• Kaddour R, Nasri N, M’rah S, Berthomieu P, Lachaâl M (2009) Comparative effect of potassium on K and Na uptake and transport in two accessions of Arabidopsis thaliana during salinity stress. C R Biologies 332:784–794
• Kanhaiya L (1996) Biochemical studies on relation to adaptability of sugarcane cultivars under saline soil. Bharatia Sug 22:23–26
• Kawano T, Kawano N, Muto S, Lapeyrie F (2001) Cation-induced superoxide generation in tobacco cell suspension culture is dependent on ion valence. Plant Cell Environ 24:1235–1241
• Kingsbury RW, Epstein E, Pearcy RW (1984) Physiological responses to salinity in selected lines of wheat. Plant Physiol 74:417–423
• Lichtenthaler HK (1988) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Method Enzymol 148:350–383
• Lopez-Climent MF, Arbona V, Perez-Clemente RM, Gomez-Cadenas A (2008) Relationship between salt tolerance and photosynthetic machinery performance in citrus. Env Exp Bot 62:176–184
• Marchner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic Press, London, p 889
• Mittova V, Guy M, Tal M, Volokita M (2002) Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii to salt-dependent oxidative stress: increased activities of antioxidant enzymes in root plastids. Free Radic Res 36:195–202
• Moreno DA, Villora G, Romero L (2003) Variations in fruit micronutrient contents associated with fertilization of cucumber with macronutrients. Sci Hortic 97:121–127
• Sairam RK, Srivastava GC, Saxena DC (2000) Increased antioxidant activity under elevated temperature: a mechanism of heat stress tolerance in wheat genotypes. Biol Plant 43:245–251
• Sairam RK, Chandrasekhar V, Srivastava GC (2001) Comparison of hexaploid and tetraploid wheat cultivars in their response to water stress. Biol Plant 44:89–94
• Satti SME, Lopez M (1994) Effect of increasing potassium levels for alleviating sodium chloride stress on the growth and yield of tomato. Commun Soil Sci Plant Anal 25:2807–2823
• Schroeder JI, Ward JM, Gassmann W (1994) Perspectives on the physiology and structure of inward-rectifying K⁺ channels in higher plants. Biophysical implications for K⁺ uptake. Annu Rev Biophys Biomol Struct 23:41–71
• Shabala S, Cuin TA (2008) Potassium transport and plant salt tolerance. Physiol Plant 133:651–669
• Singh AK, Dubey RS (1995) Changes in chlorophyll a and b contents and activities of photosystems I and II in rice seedlings induced by NaCl. Photosynthetica 31:489–499
• Song JQ, Fujiyama H (1996) Difference in response of rice and tomato subjected to sodium salinization to the addition of calcium. Soil Sci Plant Nutr 42:503–510
• Wahid A, Hameed M, Rasul E (2004) Salt-induced injury symptoms, changes in nutrient and pigment composition, and yield characteristics of mungbean. Int J Agric Biol 6:1143–1152
• Zhao GQ, Ma BL, Ren CZ (2007) Growth, gas exchange, chlorophyll fluorescence, and ion content of naked oat in response to salinity. Crop Sci 47:123–131

Uwagi

Rekord w opracowaniu