Effect of dietary supplementation with extracts of rosemary, olive leaves, pine bark and quercetin on selected performance indices of broiler chickens and microbiological status of their ileum

• Autorzy: Sierzant K. , Orda J. , Korzeniowska M. , Malicki A.
• Języki publikacji: EN

Abstrakty

EN

A total of 180 1-day-old male Hubbard Flex broiler chickens were used in a 32-day model experiment to determine the effects of dietary supplementation with quercetin (Q) and with polyphenolic extracts of rosemary (RO), olive leaves (OL) and pine bark (PB) on the performance of the birds and the microbiological status of their ileum. The chickens were randomly allocated into 9 groups: the control group (with 6 replicates, 6 birds per cage) and 8 treatment groups (with 3 replicates in each, 6 birds per cage), and fed ad libitum throughout the experimental period with a basal isoenergetic and isoprotein control diet or with the same basal diet containing two concentrations of RO, OL and PB extracts (2.50 and 5.00 g/kg), and Q (0.25 and 0.50 g/kg). The body weight gain (BWG) and the feed conversion ratio (FCR) were determined during the experiment. At day 32, two randomly selected birds from each cage were slaughtered, and 5-centimetre-long pieces of the ileum beginning from the Meckel's diverticulum were collected to analyze the number of microorganisms in the intestinal content. Chickens’ weight gain and FCR were not affected by the OL-, PB- and Q-enriched diets, but supplementation with RO significantly (P < 0.05) impaired FCR. BWG was significantly (P < 0.05) reduced when chickens were fed with mixtures containing 2.50 and 0.25 g/kg of the polyphenolic additives. The number of CFUs of intestinal microorganisms was not significantly affected (P > 0.05) by the diet modification. However, a large decrease (P > 0.05) was observed in the CFUs of coliform bacteria (up to 96%), E. coli (up to 93%), Lactobacillus spp. (up to 89%), molds and yeasts (up to 95%) and anaerobic Clostridium spp. (up to 52%) in the ileum content of chickens supplemented with the additives containing polyphenols.

Słowa kluczowe

EN

animal feeding; broiler chicken; chicken; diet; dietary supplementation; plant extract; polyphenol extract; rosemary; olive; leaf; pine bark; quercetin; DPPH method; performance index; ileum; microbiological status

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2019
• Tom: 75
• Numer: 04
• Opis fizyczny: p.247-252,fig.,ref.

Twórcy

autor

Sierzant K.

• Department of Animal Nutrition and Feed Quality, Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 38C, 51-630 Wroclaw, Poland

autor

Orda J.

• Department of Animal Nutrition and Feed Quality, Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 38C, 51-630 Wroclaw, Poland

autor

Korzeniowska M.

• Department of Animal Products Technology and Quality Management, Faculty of Biotechnology and Food Science, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 37, 51-630 Wroclaw, Poland

autor

Malicki A.

• Department of Food Hygiene and Consumer Health, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, C.K.Norwida 31, 50-375 Wroclaw, Poland

Bibliografia

• Abdel-Raouf N., Ibraheem I. B. M., Abdel-Tawab S., Naser Y. A. G.: Antimicrobial and antihyperlipidemic activities of isolated quercetin from Anabaena aequalis. J. Phycol. 2011, 47, 955-962.
• Abramovič H., Terpinc P., Generalić I., Skroza D., Klančnik A., Katalinic V., Možina S. S.: Antioxidant and antimicrobial activity of extracts obtained from rosemary (Rosmarinus officinalis) and vine (Vitis vinifera) leaves. Croat. J. Food Sci. Technol. 2012, 4, 1-8.
• Aengwanich W., Suttajit M., Srikhun T., Boonsorn T.: Antibiotic effect of polyphenolic compound extracted from tamarind (Tamarindus indica L.) seed coat on productive performance of broilers. Int. J. Poultr. Sci. 2009, 8, 749-751.
• Ahna J., Grün I. U., Mustapha A.: Effects of plant extracts on microbial growth, color change, and lipid oxidation in cooked beef. Food Microbiol. 2007, 24, 7-14.
• AOAC (2005) Official Methods of Analysis, 17edn (Arlington, VA, Association of Official Analytical Chemists).
• Bañón S, Méndez L., Almela E.: Effects of dietary rosemary extract on lamb spoilage under retail display conditions. Meat Sci. 2012, 90, 579-583.
• Barreto M. S. R., Menten J. F. M., Racanicci A. M. C., Pereira P. W. Z., Rizzo P. V.: Plant extracts used as growth promoters in broilers. Brazil. J. Poultr. Sci. 2008, 10, 109-115.
• Bernard F. X., Sablé S., Cameron B., Provost J., Desnottes J. F., Crouzet J., Blanche F.: Glycosylated flavones as selective inhibitors of topoisomerase IV. Antimicrobial Agents Chemotherap. 1997, 41, 992-998.
• Chen B. T., Li W. X., He R. R., Li Y. F., Tsoi B., Zhai Y. J., Kurihara H.: Antiinflammatory effects of a polyphenols-rich extract from tea (Camellia sinensis) flowers in acute and chronic mice models. Oxidat. Med. Cell. Longev. 2012, doi: 10.1155/2012/537923.
• Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. Off. J. Europ. Comm. 358, 1-28.
• Hirai I., Okuno M., Katsuma R., Arita N., Tachibana M., Yamamoto Y.: Characterization of anti-Staphylococcus aureus activity of quercetin. Int. J. Food Sci. Technol. 2010, 45, 1250-1254.
• Huyghebaert G., Ducatelle R., van Immerseel F.: An update on alternatives to antimicrobial growth promoters for broilers. Vet. J. 2011, 187, 182-188.
• Korukluoğlu M, Şahan Y., Yiğit A.: In-vitro antibacterial activity of olive leaf (Olea europea L.) extracts and their chemical characterization. Adnan Menderes University, Proc. 4th AACD Congress, 29.09-03.10.2004, Kutadasy- Aydin, Turkey 291, 563-565.
• Kumarappan C. T., Mandal S. C.: Antitumor activity of polyphenolic extract of Ichnocarpus frutescens. Exp. Oncol. 2007, 9, 94-101.
• Lee K. A., Moon S. H., Kim K. T., Mendonca A. F., Paik H. D.: Antimicrobial effects of various flavonoids on Escherichia coli O157:H7 cell growth and lipopolysaccharide production. Food Sci. Biotechnol. 2010, 19, 257-261.
• Markin D., Duek L., Berdicevsky I.: In vitro antimicrobial activity of olive leaves. Mycoses 2002, 46, 132-136.
• Martha I., Ardila Q., Andrés F., Vargas A., Jorge E., Pérez C., Luis F., Mejía G.: Ensayo preliminar de la actividad antibacteriana de extractos de Allium sativum, Coriandrum sativum, Eugenia caryophyllata, Origanum vulgare, Rosmarinus officinalis Y Thymus vulgaris frente a Clostridium perfringens. Biosalud 2009, 8, 47-57.
• Mostafa H. A. M., El-Bakry A. A., Eman A. A.: Evaluation of antibacterial activity of different plant parts of Rumex vesicarius L. at early and late vegetative stages of growth. Int. J. Pharm. Pharmaceut. Sci. 2012, 4, Suppl. 4, 426-435.
• Ojeda-Sana A. M., Repetto V., Moreno S.: Carnosic acid is an efflux pumps modulator by dissipation of the membrane potential in Enterococcus faecalis and Staphylococcus aureus. World J. Microbiol. Biotechnol. 2013, 29, 137-144.
• Ok-Hwan L., Boo-Yong L.: Antioxidant and antimicrobial activities of individual and combined phenolics in Olea europaea leaf extract. Biores. Technol. 2010, 101, 3751-3754.
• Park B. S.: Effects of pitamin on growth performance, carcass characteristics and cecal microflora of broiler chicken. J. Env. Biol. 2011, 32, 585-590.
• Pereira A. P., Ferreira I. C. F. R., Marcelino F., Valentão P., Andrade P. B., Seabra R., Estevinho L., Bento A., Pereira J. A.: Phenolic compounds and antimicrobial activity of olive (Olea europaea L. Cv. Cobrançosa) leaves. Molecules 2007, 12, 1153-1162.
• PN-EN 15787:2009. Fodder. Detection and quantity determination of Lactobacillus spp. (in Polish).
• PN-EN ISO 7937:2005. Food and fodder microbiology. Horizontal methodology for Clostridium perfringens quantity determination. Colony counting method (in Polish).
• PN-ISO 21527-1:2009. Food and fodder microbiology. Horizontal methodology for yeasts and molds quantity determination. Part 1: Colony counting method in products with water activity higher than 0.95 (in Polish).
• PN-ISO 4832:2007. Food and fodder microbiology. Horizontal methodology for coliform bacteria quantity determination. Petri dish method (in Polish).
• PN-ISO 7251:2006. Food and fodder microbiology. Horizontal methodology for Escherichia coli detection and quantity determination. Methods for the most probable number of formed colonies (in Polish).
• Regulation (EC) No 1831/2003 of The European Parliament And of The Council of 22 September 2003 on additives for use in animal nutrition (Text with EEA relevance), 18.10.2003. Offic. J. EU. 268, 29-43.
• Shu Y., Liu Y., Li L., Feng J., Lou B., Zhou X., Wu H.: Antibacterial activity of quercetin on oral infectious pathogens. Afric. J. Microbiol. Res. 2011, 5, 5358-5361.
• Sierżant K., Pyrkosz-Biardzka K., Gabrielska J.: Antioxidant properties of natural polyphenolic extracts from selected plants in model systems. Food Sci. Technol. Quality 2012, 85, 41-53.
• StatSoft, Inc. (2011). Statistica (data analysis software system), version 10. www.statsoft.com.
• Šperňáková D., Máté D., Różaňska H., Kováč G.: Effects of dietary rosemary extract and α-tocopherol on the performance of chickens, meat quality, and lipid oxidation in meat storaged under chilling conditions. Bull. Vet. Instit. Pulawy 2007, 51, 585-589.
• Torras M. A. C., Faura C. A., Schönlau F., Rohdewald P.: Antimicrobial activity of Pycnogenol®. Phytotherap. Res. 2005, 19, 647-648.
• Travassoli S., Djomeh Z. E.: Total phenols, antioxidant potential and antimicrobial activity of methanol extract of rosemary (Rosmarinus officinalis L.). Global Vet. 2011, 7, 337-341.
• Viveros A., Chamorro S., Pizarro M., Arija I., Centeno C., Brenes A.: Effects of dietary polyphenol-rich grape products on intestinal microflora and gut morphology in broiler chicks. Poultr. Sci. 2011, 90, 566-578.
• Wang L., Piao X. L., Kim S. W., Piao X. S., Shen Y. B., Lee H. S.: Effects of Forsythia suspensa extract on growth performance, nutrient digestibility, and antioxidant activities in Broiler chickens under high ambient temperature. Poultr. Sci. 2008, 87, 1287-1294.
• WPSA: European Table of Energy Values for Poultry Feedstuffs. European Federation of Branches of the World’s Poultry Science Association 1989.

Identyfikatory

DOI

10.21521/mw.6143