Isolation of biosynthesis related transcripts of 2,3,5,4'-tetrahydroxy stilbene-2-O-beta-D-glucoside from Fallopia multiflora by suppression subtractive hybridization

• Autorzy: Zhao W. , Sheng S. , Liu Z. , Lu D. , Zhu K. , Li X. , Zhao S. , Yao Y.
• Języki publikacji: EN

Abstrakty

EN

2,3,5,4'-tetrahydroxy stilbene-2-O-ß-D-glucoside (THSG) exerts multiple pharmacodynamic actions, found in Fallopia multiflora, but the biosynthesis pathway of THSG is still unclear. To clear this ambiguity, we constructed suppression subtractive hybridization (SSH) libraries to screen the genes involved in THSG biosynthesis from two F. multiflora varieties, which vary significantly in THSG content. Twelve non-redundant differentially expressed sequence tags were obtained and the full lengths of 4 unreported fragments were amplified by rapid amplification of cDNA ends. We totally got 7 fulllength transcripts, and all of them were aligned to the transcriptome and digital gene expression tag profiling database of four F. multiflora tissues (root, stem and leaf from Deqing F. multiflora and another root from Chongqing F. multiflora; data unpublished) using local BLAST. The results showed that there was a significant, organ specific difference in the expression of fragments and full-length sequences. All the sequences were annotated by aligning to nucleotide and protein databases. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that THSG biosynthesis was correlated with multiple life activities.

Słowa kluczowe

EN

isolation; biosynthesis; transcriptome; 2,3,5,4'-tetrahydroxy stilbene-2-O-beta-D-glucoside; Fallopia multiflora; hybridization; gene expression; medicinal plant; Chinese herb

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2014
• Tom: 83
• Numer: 2
• Opis fizyczny: p.147-157,fig.,ref.

Twórcy

autor

Zhao W.

• School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, People’s Republic of China
• Department of Pharmacy, General Hospital of Guangzhou Military Command, Guangzhou 510010, People’s Republic of China

autor

Sheng S.

• Department of Pharmacy, General Hospital of Guangzhou Military Command, Guangzhou 510010, People’s Republic of China

autor

Liu Z.

• Department of Pharmacy, General Hospital of Guangzhou Military Command, Guangzhou 510010, People’s Republic of China

autor

Lu D.

• Department of Pharmacy, General Hospital of Guangzhou Military Command, Guangzhou 510010, People’s Republic of China

autor

Zhu K.

• School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, People’s Republic of China
• Department of Pharmacy, General Hospital of Guangzhou Military Command, Guangzhou 510010, People’s Republic of China

autor

Li X.

• School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, People’s Republic of China
• Department of Pharmacy, General Hospital of Guangzhou Military Command, Guangzhou 510010, People’s Republic of China

autor

Zhao S.

• Department of Pharmacy, General Hospital of Guangzhou Military Command, Guangzhou 510010, People’s Republic of China

autor

Yao Y.

• School of Life Sciences, Guangzhou University, Guangzhou 510006, People’s Republic of China

Bibliografia

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Identyfikatory

DOI

10.5586/asbp.2014.012