Flow cytometric determination of the expression of gp 51 protein of bovine leukaemia virus in experimentally infected sheep

• Autorzy: Szczotka M.
• Języki publikacji: EN

Abstrakty

EN

The study was performed on lambs experimentally infected with bovine leukaemia virus (BLV). The presence of BLV antibodies in sera of infected animals was detected by agar gel immunodiffusion test and ELISA. Proviral DNA was detected by PCR and nested PCR. Dual-colour flow cytometry analysis was performed with the use of specific monoclonal antibodies against lymphocyte CD markers and gp51 viral envelope protein, followed by incubation with fluorescent-labelled secondary antibodies conjugated with FITC or PE. Gp51 viral envelope protein was detected in tumours caused by BLV infection. The BLV infection resulted in depletion of CD4+ lymphocytes, increase in CD8+ lymphocytes, and decrease in CD4+ to CD8+ ratio in infected sheep. Proliferation of IgM+ CD19+ cells was also detected. These cells had an immature character without tendency to differentiate, and their vitality was prolonged. Flow cytometry enabled detection of gp51 expression in sheep blood lymphocytes at the early stages of the infection, before detection of serum antibodies using ELISA.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2014
• Tom: 58
• Numer: 3
• Opis fizyczny: p.353-361,fig.,ref.

Twórcy

autor

Szczotka M.

• Department of Biochemistry, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Adam E., Kerkhofs P., Mammerickx M., Burny A., Droogmans L., Willems L.: Involvement of the cyclic AMP- responsive element binding protein in bovine leukemia virus expression in vivo. J Virol 1994, 68, 5845-5853.
• 2. Aida Y., Miyasaka M., Okada K.., Onuma M., Kogure S., Suzuki M., Minoprio P., Levy D., Ikawa Y.: Further phenotypic characterization of target cells for bovine leukemia virus experimental infection in sheep. Am J Vet Res 1989, 50, 1946- 1951.
• 3. Aida Y., Okada K., Amanuma H.: Phenotype and ontogeny of cells carrying a TAA that is expressed of bovine leukemia virus induced lymphosarcoma. Cancer Res 1993, 53, 429-437.
• 4. Balič D., Lojkič I., Perskič M., Bedekovič J.: Identification of a new genotype of bovine leukaemia virus. Arch Virol 2013, 157, 1281-1290.
• 5. Birkebak T.A., Palmer G.H., Davis W.C., Knowles D.P., McElwain T.F.: Association of gp51 expression and persistent CD5+ B-lymphocytes lymphocytosis in sheep infected with BLV. Leukemia 1994, 8, 1890-1899.
• 6. Cantor G.H., Pritchard S.M., Dequiedt F., Willems L., Kettmann R., Davis W.C.: CD5 is dissociated from the B-cell receptor in B- cells from bovine leukemia virus infected persistently lymphocytotic cattle: consequences to B-cell receptor - mediated apoptosis. J Virol 2001, 75, 1689-1696.
• 7. Chevallier N., Berthelemy M., Le Rhun D., Laine V., Levy D., Schwartz-Cornil I.: Bovine leukemia virus-induced lymphocytosis and increased cell survival mainly involve the CD11b+ B lymphocyte subset in sheep. J Virol 1998, 72, 4413-4420.
• 8. Clevers H., Mac Hugh N., Bensaid A.: Identification of a bovine surface antigen uniquely expressed on CD4-CD8-T cell receptor gamma/delta T+ lymphocytes. Eur J Immunol 1990, 20, 809-817.
• 9. Coffin J.M.: Retroviridae: The viruses and their replication. In: Fields Virology, Lippincot-Raven Publishers, Philadelphia, 1997, pp. 641-671.
• 10. Cockerell G.L., Parodi A.L., Levy D.: Immunocompetence of sheep experimentally infected with bovine leukemia virus. Vet Immunol Immunopathol 1986, 13, 189-196.
• 11. Depelchin A., Letesson J.J., Lostrie-Trussart N., Mammerickx M., Portetelle D., Burny A.: Bovine leukemia virus (BLV) infected B- cells express a marker similar to the CD5 T- cell marker. Immunol Lett 1989, 29, 69-76.
• 12. Djilali S., Parodi A.L.: The BLV- induced leukemia- lymphosarcoma complex in sheep. Vet Immunol Immunopathol 1989, 22, 233-244.
• 13. Gatei M., Brandon R., Naif H., McLennan M., Daniel R., Levin M.: Changes in B and T cell subsets in bovine leukemia virus-infected cattle. Vet Immunol Immunopathol 1989, 23, 139-147.
• 14. Hidalgo B., Bonilla A.: Lymphoproliferation assays in cattle naturally infected with bovine leukemia virus (BLV) and bovine immunodeficiency virus (BIV). J Vet Med B 1996, 43, 325-332.
• 15. Jensen W.A., Rovnak J., Cockerell G.L.: In vivo transcription of the bovine leukemia virus tax/rex region in normal and neoplastic lymphocytes of cattle and sheep. J Virol 1991, 65, 2484-2490.
• 16. Jensen W.A., Sheehy S.E., Fox M.H., Davis W.C., Cockerell G.L.: In vitro expression of bovine leukemia virus in isolated B- lymphocytes of cattle and sheep. Vet Immunol Immunopathol 1990, 26, 333-342.
• 17. Kettmann R., Dechamps J., Cleuter Y., Ghysdael J., Mammerickx M.: Distribution of bovine leukemia virus proviral DNA sequences in tissue of animals with enzootic bovine leucosis. Leuk Res 1998, 2, 23-32.
• 18. Klener P., Szynal Y., Cleuter M., Merimi H., Duvillier F.: Insightes into gene expression changes impacting B-cell transformation: cross-species mieroarray analysis of bovine leukemia virus tax-responsive genes in bovine B-cells. J Virol 2006, 55, 690-695.
• 19. Lewin H.A., Bernoco D.: Evidence for BoLA-linked resistance and susceptibility to subclinical progression of bovine leukaemia virus infection. Anim Genet 1986, 17, 197-207.
• 20. Llames L., Goyache J., Domenech A., Montana A.V., Suarez G., Gomez-Lucia E.: Cellular distribution of bovine leukemia virus proteins gp51SU, Pr72(env), and Pr66 (gag-pro) in persistently infected cells. Virus Res 2001, 79, 45-57.
• 21. Mamoun R.Z., Morrisson M., Rebeyrotte B., Busetta B., Kettmann R., Guilleman B.: Sequence variability of bovine leukemia virus env gene and its relevance to the structure and antigenicity of the glycoproteins. J Virol 1990, 64, 4180-4188.
• 22. Meirom R., Moss S., Brenner J.: Bovine leukemia virus gp51 antigen expression is associated with CD5 and IgM markers on infected lymphocytes. Vet Immunol Immunopathol 1997, 59, 113-119.
• 23. Mirsky M.L., Olmstead C.A., Da Y., Levin H.: The prevalence of proviral bovine leukemia virus in peripheral blood mononuclear cells at two subclinical stages of infection. J Virol 1996, 70, 2178-2183.
• 24. Mirsky M.L., Olmstead C., Da Y., Lewin H.: Reduced bovine leukemia virus proviral load in genetically resistant cattle. Animal Genet 1998, 29, 245-252.
• 25. Powers M., Radke K.: Activation of bovine leukemia virus transcription in lymphocytes from infected sheep: rapid transition through early to late gene expression. J Virol 1992, 66, 4769-4777.
• 26. Sagata N., Yasanuga T., Tsuzuku-Kawamura J., Ohishi K., Ogawa Y., Ihawa I.: Complete nucleotide sequence of the genome of bovine leukemia virus; its evolutionary relationship to other retroviruses. Proc Nat Acad Sci USA 1985, 82, 677-682.
• 27. Schwartz I., Bensaid A., Polack B., Perrin B.: In vivo leukocyte tropism of bovine leukemia virus in sheep and cattle. J Virol 1994, 68, 4589-4596.
• 28. Stec J., Grundboeck J., Szczotka M., Reichert M., Rulka J., Kozaczyńska B.: Effect of mitogens on the in vitro stimulation of lymphocytes from normal and BLV-infected cattle. Bull Vet Inst Pulawy 1993, 33, 29-35.
• 29. Stone D.M., McElwain T.F., Davis W.C.: Enhanced B- lymphocyte expression of IL-2R alpha associated with T lymphocytosis in BLV-infected persistently lymphocytotic cows. Leukemia 1994, 8, 1057-1061.
• 30. Stone D.M., Norton L.K., Davis W.C.: Modulation of bovine leukemia virus associated spontaneous lymphocyte proliferation by monoclonal antibodies to lymphocyte surface molecules. Clin Immunol Immunopathol 1997, 83, 156-164.
• 31. Szczotka M.: Acid phosphatase and non-specific alpha-naphthyl esterase activities in lymphocytes of sheep experimentally infected with bovine leukemia virus (BLV) and in trials of active immunization. Thesis. National Veterinary Research Institute, Pulawy, Poland, 1988.
• 32. Takashima I., Olson C., Driscoll D.M., Baumgartener L.E.: B-lymphocytes and T lymphocytes in three types of bovine lymphosarcoma. J Natl Cancer Inst 1977, 59, 1205-1209.
• 33. Trainin Z., Brenner J., Meirom R., Ungar-Waron H.: Detrimental effect of bovine leukemia virus (BLV) on the immunological state of cattle. Vet Immunol Immunopathol 1996, 54, 293-302.
• 34. Uchiyama T.: Human T-cell leukemia virus type I (HTLV-I) and human diseases. Ann Rev Immunol 1997, 15, 15-37.
• 35. Udina I.G., Karamysheva E.E., Turkova S.O., Orlova A.R., Sulimova G.E..: Genetic mechanisms of resistance and susceptibility to leukemia in Ayrshire and black pied cattle breeds determined by allelic distribution of gene Bola-DRB3. Genetika 2003, 39, 383-96.
• 36. Xu A., Van Ejik M., Park C., Levin H.A.: Polymorphism in BoLa-DRB3 ekson 2 correlates with resistance to persistent lymphocytosis caused by bovine leukaemia virus. J Immunol 1993, 12, 6977-6981.

Identyfikatory

DOI

10.2478/bvip-2014-0055