Aeromonas hydrophila subsp. dhakensis – a causative agent of gastroenteritis imported into the Czech Republic

• Autorzy: Sedlacek I. , Krejci E. , Andelova A. , Sedlackova M. , Porazilova I. , Holochova P.
• Języki publikacji: EN

Abstrakty

EN

Out of the twenty-one A. hydrophila complex isolates obtained during a routine examination of human diarrhoeal faeces, two A. hydrophila subsp. dhakensis isolates (P1097 = CCM 7329 and P1165) were successfully identified by ribotyping. The correct taxonomic position of the A. hydrophila subsp. dhakensis CCM 7329 was verified by cpn60 sequencing (GeneBank accession number HM536193). The remaining A. hydrophila complex isolates were identified as A. hydrophila subsp. hydrophila. The ability of biochemical tests and fatty acid methyl ester analysis to reliably discern both A. hydrophila subsp. dhakensis and A. hydrophila subsp. hydrophila was limited. In contrast to the A. hydrophila subsp. hydrophila, the faecal isolates of A. hydrophila subsp. dhakensis did not produce acid from arbutin. When compared in a two-dimensional plot, the A. hydrophila subsp. dhakensis faecal isolates contained higher amounts of the two minor fatty acids C13:0 and C17:1 ω8c than the A. hydrophila subsp. hydrophila reference strain. This is the first detected occurrence of the less frequent A. hydrophila subsp. dhakensis in our region and ribotyping was proved as a suitable method for the identification of A. hydrophila subsp. dhakensis.

• Wydawca: -
• Czasopismo: Annals of Agricultural and Environmental Medicine
• Rocznik: 2012
• Tom: 19
• Numer: 3
• Opis fizyczny: p.409-413,fig.,ref.

Twórcy

autor

Sedlacek I.

• Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Brno, Czech Republic

autor

Krejci E.

• Institute of Public Health, Centre for Clinical Laboratories, Ostrava, Czech Republic

autor

Andelova A.

• Institute of Public Health, Centre for Clinical Laboratories, Ostrava, Czech Republic

autor

Sedlackova M.

• Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Brno, Czech Republic

autor

Porazilova I.

• Institute of Public Health, Centre for Clinical Laboratories, Ostrava, Czech Republic

autor

Holochova P.

• Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Brno, Czech Republic

Bibliografia

• 1. Sedláček I, Jakšl V, Přepechalová H. Identification of aeromonads from water sources. Epidemiol Mikrobiol Imunol. 1994; 43: 61-66 (in Czech).
• 2. Janda JM, Guthertz LS, Kokka RP, Shimada T. Aeromonas species in septicaemia: laboratory characteristics and clinical observations. Clin Infect Dis. 1994; 19: 77-83.
• 3. Grobusch MP, Gobels K, Teichmann D. Cellulitis and septicaemia by Aeromonas hydrophila acquired at home. Infection. 2001; 29: 109-110.
• 4. Janda JM. Recent advances in the study of the taxonomy, pathogenicity, and infectious syndromes associated with genus Aeromonas. Clin Microbiol Rev. 1991; 4: 397-410.
• 5. Euzéby JP. List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int J Syst Bacteriol. 1997; 47: 590-592.(List of Prokaryotic names with Standing in Nomenclature. (http://www.bacterio.net, access: 2011.08.10).
• 6. Martin-Carnahan A, Joseph SW. Genus I. Aeromonas. In: Garrity GM, Brenner DJ, Krieg NR, Staley JT. Bergey’s Manual of SystematicBacteriology. Second edition. Volume Two. The Proteobacteria. Part BThe Gammaproteobacteria. USA, Springer, 2005. p. 557-578.
• 7. Miyake M, Iga K, Izumi C, Miyagawa A, Kobashi Y, Konishi T. Rapidly progressive pneumonia due to A. hydrophila shortly after neardrowning. Intern Med. 2000; 39: 1128-1130.
• 8. Miñana-Galbis D, Urbizu-Serrano A, Farfan M, Fusté MC, Loren JG. Phylogenetic analysis and identification of Aeromonas species based on sequencing of the cpn60 universal target. Int J Syst Evol Microbiol. 2009; 59: 1976-1983.
• 9. Huys G, Kämpfer P, Albert MJ, Kühn I, Denys R, Swings J. Aeromonas hydrophila subsp. dhakensis subsp. nov., isolated from children withdiarrhoea in Bangladesh, and extended description of Aeromonashydrophila subsp. hydrophila (Chester 1901) Stainer 1943. Int J Syst Evol Microbiol. 2002; 52: 705-712.
• 10. Huys G, Pearson M, Kämpfer P, Denys R, Cnockaert M, Inglis V, Swings J. Aeromonas hydrophila subsp. ranae subsp. nov., isolated from septicaemic farmed frogs in Thailand. Int J. Syst Evol Microbiol.2003; 53: 885-891.
• 11. Abbott SL. Aeromonas. In: Murray PR, Baron EJ, Jorgenson JH, Pfaller MA, Yolken RH. Manual of Clinical Microbiology, 8th edition.Washington, D.C., ASM Press, 2003. p. 701-705.
• 12. Abbott SL, Cheung WKW, Janda JM. The genus Aeromonas: biochemical characteristics, atypical reactions and phenotypic identification schemes. J Clin Microbiol. 2003; 41: 2348-2357.
• 13. Abbott SL, Seli LS, Catino M Jr., Hartley MA, Janda JM. Misidentification of unusual Aeromonas species as members of genus Vibrio: a continuingproblem. J Clin Microbiol. 1998; 36: 1103-1104.
• 14. Andĕlová A, Porazilová I, Krejčí E. Aeromonas agar is a useful selective medium for isolating aeromonads from faecal samples. J Med Microbiol.2006; 55: 1605-1606.
• 15. Cowan ST. Cowan and Steel’s manual for the identification of medical bacteria. Cambridge, University Press, 1974. p 150-171.
• 16. Páčová Z, Kocur M. New medium for detection of esterase and gelatinase activity. Zbl Bakt Hyg A. 1984; 258: 69-73.
• 17. Švec P, Sedláček I, Pantůček R, Devriese LA, Doškař J. Evaluation of ribotyping for characterization and identification of Enterococcushaemoperoxidus and Enterococcus moraviensis strains. FEMS MicrobiolLett. 2001; 203: 23-27.
• 18. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrixchoice. Nucleic Acids Res. 1994; 22: 4673-4680.
• 19. Tamura K, Dudley J, Nei M, Kumar S. Phylogenetic and molecular evolutionary analyses were conducted using MEGA version 4. Mol BiolEvol. 2007; 24: 1596-1599.
• 20. Saitou N, Nei M. The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4: 406-425.
• 21. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101. MIDI, Newark, DE, USA 2001.(http://www.midi-inc.com/media/pdfs/TechNote_101.pdf).
• 22. MIDI, Inc. Library Generation Software user’s manual, version 4.5. MIDI, Inc., Newark, DE, USA 2002.
• 23. Huys G, Vancanneyt M, Coopman R, Janssen P, Falsen E, Altwegg M, Kersters K. Cellular fatty acid composition as a chemotaxonomicmarker for differentiation of phenospecies and hybridization groups inthe genus Aeromonas. Int J Syst Bacteriol. 1994; 44: 651-658.
• 24. Kühn I, Albert MJ, Ansaruzzaman M, Bhuiyan NA, Alabi SA, Islam MS, Neogi PK, Huys G, Janssen P, Kersters K, Möllby R. Characterizationof Aeromonas spp. isolated from humans with diarrhea, from healthycontrols, and from surface water in Bangladesh. J Clin Microbiol.1997; 35: 369-373.
• 25. Albert M, Faruque ASG, Faruque SM, Sack RB, Mahalanabis D. Casecontrol study of enteropathogens associated with childhood diarrhoeain Dhaka, Bangladesh. J Clin Microbiol. 1999; 37: 3458-3464.
• 26. Farmer III JJ, Arduino MJ, Hickman-Brenner FW. The Genera Aeromonas and Plesiomonas. In: Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E. The Prokaryotes, A Handbook on the Biology of Bacteria: An Evolving Electronic Resource for the Microbiological Community. 3rd edition. Volume 6: Proteobacteria: Gamma subclass. New York, Springer-Verlag, 2006. p. 564-596. (http://link.springer-ny.com/link/service/books/10125/).
• 27. Ørmen Ø, Granum PE, Lassen J, Figueras MJ. Lack of agreement between biochemical and genetic identification of Aeromonas spp.APMIS. 2005; 113: 203-207. 413

Uwagi

rekord w opracowaniu