The aim of this study was to investigate the capacity of Streptococcus suis strains to form biofilms and to evaluate the antimicrobial activity of Penicillin G and N-acetylcystein (NAC) on both S. suis sessile and planktonic forms. Only non-typeable isolates of S. suis were correlated with a greater biofilm formation capacity. The MCI of Penicillin G and NAC required for inhibiting biofilm growth were higher than the required concentration for inhibiting planktonic growth. The combinations of NAC and Penicillin G showed a strong synergistic activity that inhibited biofilm formation and disrupted the pre-formed biofilm of S. suis.
Groupe de Recherche sur les Maladies Infectieuses du Porc and Centre de Recherche en Infectiologie Porcine, Faculte de Medecine Veterinaire, Universite de Montreal, St-Hyacinthe, Canada
Bibliografia
Aslam S., B.W Trautner, V. Ramanathan and R.O. Darouiche. 2007. Combination of tigecycline and N-acetylcysteine reduces biofilm-embedded bacteria on vascular catheters. Antimicrob. Agents Chemother. 51: 1556–1558.
Bonifait L., L. Grignon and D. Grenier. 2008. Fibrinogen induces biofilm formation by Streptococcus suis and enhances its antibiotic resistance. Appl. Environ. Microbiol. 74(15): 4969–4972.
Bonifait L., M. Gottschalk and D. Grenier. 2010. Cell surface characteristics of nontypeable isolates of Streptococcus suis. FEMS Microbiol. Lett. 311(2): 160–166.
Clinical and Laboratory Standards Institute (CLSI). 2008. Performance standards for antimicrobial susceptibility testing. 18th Informational supplement, M100-S18. Wayne, PA.
Gottschalk M., M. Segura and J. Xu. 2007. Streptococcus suis infections in humans: the Chinese experience and the situation in North America. Anim. Health Res. Rev. 8: 29–45.
Grenier D., L. Grignon and M. Gottschalk. 2009. Characterisation of biofilm formation by a Streptococcus suis meningitis isolate. Vet. J. 179: 292–295.
Guo D., L. Wang and L. Chengping. 2012. In vitro biofilm forming potential of Streptococcu suis isolated from human and swine in China. Braz. J. Microbiol. 43(3): 993–1004.
Hajdu S., A. Lassnigg, W. Graninger, A.M. Hirschl and E. Presterl. 2009. Effects of vancomycin, daptomycin, fosfomycin, tigecyclin and ceftriaxone on Staphylococcus epidermidis biofilms. J. Orthop Res. 27: 1361–1365.
Higgins R. and M. Gottschalk. 1990. An update on Streptococcus suis identification. J. Vet. Diagn. Invest. 2: 249–252.
Huaijie H., H. Wenjia, L. Peng, L. Qingyu, Z. Xiaotao, J. Hua, W. Yanzi, Z. Xin, Z. Yuling, L. Jianchun and others. 2013. Effect of licochalcone A on growth and properties of Streptococcus suis. PLoS One 8(7): e67728.
Huang Y.T., L.J. Teng, S.W. Ho and P.R. Hsueh. 2005. Streptococcus suis infection. J. Microbiol. Immunol. Infect. 38: 306–313.
Hui A.C., K.C. Ng, P.Y. Tong, V. Mok and K.M. Chow. 2005. Bacterial meningitis in Hong Kong:10-years’ experience. Clin. Neurol. Neurosurg. 107: 366–370.
Lun Z.R., Q.P. Wang, X.G. Chen, A. Li and X.Q. Zhu. 2007. Streptococcus suis: anemerging zoonotic pathogen. Lancet Infect. Dis. 7: 201–209.
Mackay M.L., K. Milne and I.M. Gould. 2000. Comparison of methods for assessing synergic antibiotic interactions. Int. J. Antimicrob. Agents. 15(2): 125–129.
Marchese A., M. Bozzolasco, L. Gualco, E.A. Debbia, G.C. Schito and A.M. Schito. 2003. Effect of fosfomycin alone and in combination with N-acetylcysteine on E. coli biofilms. Int. J. Antimicrob Agents. 22: 95–100.
Marois C., M. Le Devendec and M. Gottschalk. 2007. Detection and molecular typing of Streptococcus suis in tonsils from live pigs in France. Can. J. Vet. Res. 71(1): 14–22.
Merle E., H.C. Olson, W.M. Douglas, A.G. Buret and R. Ronald. 2012. Read biofilm bacteria: formation and comparative susceptibility to antibiotics. Can. J. Vet. Res. 66:86–92.
Schwandt L.Q., W. Van, I. Stokroos, M. der Van, H.J. Busscher and F.W. Albers. 2004. Prevention of biofilm formation by dairy products and N-acetylcysteine on voice prosthees in an artificial throat. Acta Otolaryngol. 124(6): 726–731.
Wang K., W. Fan, H. Wisselink and C. Lu. 2011. The cps locus of Streptococcus suis serotype 16: Development of a serotype-specific PCR assay. Vet. Microbiol. 153: 403–406.